KSHV ORF45 Antibody (YA4607)

Cat. No.: HY-P84910: Data Sheet COA
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KSHV ORF45 Antibody (YA4607) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KSHV ORF45.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

KSHV ORF45 Antibody (YA4607) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KSHV ORF45.

Host

Mouse

Clonality

Monoclonal Antibody

Species Reactivity

KSHV

SwissProt ID

F5HDE4

Gene ID

4961474 [NCBI]

Immunogen

Purified recombinant fragment of KSHV ORF45 aa 65-214.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Prevents the establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of host interferon regulatory factor 7/IRF7, a transcription factor critical for the induction of interferons alpha and beta (PubMed:11943871, PubMed:20485504, PubMed:22787218). Mechanistically, ORF45 competes with the associated IRF7 and inhibits its phosphorylation by IKBKE or TBK1 by acting as an alternative substrate (PubMed:11943871, PubMed:22787218). Acts as an activator of the NLRP1 inflammasome via interaction with the N-terminal part of host NLRP1: interaction promotes translocation of the N-terminal part of NLRP1 into the nucleus, relieving autoinhibition of the NLRP1 inflammasome and leading to its activation (PubMed:35618833). Also plays a role in promoting the late transcription and translation of viral lytic genes by constitutively activating host extracellular signal-regulated kinase (ERK)-p90 ribosomal S6 kinase/RPS6KA1 (PubMed:30842327). In addition, supports the viral replication cycle by modulating host p53/TP53 signaling pathway (PubMed:34523970). Interacts with host p53/TP53 and prevents its interaction with the deubiquitinase USP7, leading to sequestration of P53/TP53 in the host cytoplasm thereby diminishing its transcriptional activity (PubMed:34523970)

Subcellular Localization：Virion tegument; Host cytoplasm; Host nucleus; Host Golgi apparatus

Subunit：Interacts with host IRF7 (PubMed:11943871). Interacts with host RPS6KA1 (PubMed:30842327). Interacts with host RAB11FIP5; this interaction results in the lysosomal degradation of ORF45 and the inhibition of viral particle release (PubMed:33315947). Interacts with host p53/TP53; this interaction down-regulates p53/TP53 signaling pathway (PubMed:34523970). Interacts with the N-terminal part of host NLRP1; relieving autoinhibition of the NLRP1 inflammasome (PubMed:35618833)

Synonyms

ORF45

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)