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Cadherin 16 Antibody (YA5689)

(Synonyms: Cadherin-16; Kidney-specific cadherin; Ksp-cadherin; Kidney specific cadherin antibody)
Customer Review

Based on 1 Customer Validation

Cadherin 16 Antibody (YA5689) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cadherin 16.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cadherin 16 Antibody (YA5689)
  • Cadherin 16 Antibody (YA5689) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-400 1:50-200 1:500-5000

Product Details

Description

Cadherin 16 Antibody (YA5689) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Cadherin 16.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Calculated Molecular Weight Predicted band size: 90 kDa;
Immunogen

Synthesized peptide derived from human KSP-Cadherin AA range:1-100

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cadherin 16 Antibody (YA5689)
    Immunohistochemical analysis of paraffin-embedded human kidney using Cadherin 16 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85997, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Cadherin 16 is a Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Kidney specific

  • Isoforms & Post-Translational Modification

    O75309 has 4 isomers: O75309-1: 89923 Da (predicted); O75309-2: 87614 Da (predicted); O75309-3: 79196 Da (predicted); O75309-4: 85774 Da (predicted).

  • SwissProt ID

    O75309

  • Gene ID
    1014 [NCBI]
  • Synonyms

    Cadherin-16; Kidney-specific cadherin; Ksp-cadherin; Kidney specific cadherin antibody

Cadherin 16 Antibody (YA5689) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney using Cadherin 16 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85997, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cadherin 16 Antibody (YA5689) Product Image
    1/16
  • Experimental Validation Results for Cadherin 16 Antibody (YA5689)
  • Cadherin 16 Antibody (YA5689) Product Image
  • Experimental Validation Results for Cadherin 16 Antibody (YA5689)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney using Cadherin 16 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85997, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cadherin 16 Antibody (YA5689)
  • Cadherin 16 Antibody (YA5689) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney using Cadherin 16 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P85997, 1/400) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cadherin 16 Antibody (YA5689) Product Image IHC-P