MAGE-3 Antibody (YA4021)(PBS only)

Cat. No.: HY-P84324A: Fiche technique User Guide for Antibodies
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MAGE-3 Antibody (YA4021) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to MAGE-3.

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Size		Stock
50 μL		Obtenir un devis
100 μL		Obtenir un devis

* Veuillez sélectionner la quantité avant d'ajouter des articles.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description
Références

Description

MAGE-3 Antibody (YA4021) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to MAGE-3.

Host

Mouse

Masse moléculaire

Predicted band size: 35 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P43357

Gene ID

4102 [NCBI]

Immunogen

Purified recombinant fragment of human MAGE-3 (AA: FLWGPRALVC) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureté	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy (PubMed:20864041, PubMed:31267705). May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex (PubMed:17942928, PubMed:20864041). May play a role in embryonal development and tumor transformation or aspects of tumor progression (PubMed:17942928, PubMed:20864041). In vitro promotes cell viability in melanoma cell lines (PubMed:17942928). Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes (PubMed:8113684)

Expression：
Tissue_specificity:This gene is expressed in various types of tumors, such as melanoma, head and neck squamous cell carcinoma, lung cancer, and breast cancer, but not in normal tissues except for the testes and placenta. It has never been expressed in kidney tumors, leukemia, and lymphoma.

Subunit：Interacts with TRIM28

Synonyms

HIP8; HYPD; CT1.3; MAGE3; MAGEA6

Documentation

User Guide for Antibodies (1077 KB)

Références

[1]. /biology-dictionary/hela-cell.html [Content Brief]