MCM6 Antibody (YA1146)
(Synonyms: minichromosome maintenance complex component 6; Mis5; P105MCM; MCG40308)MCM6 Antibody (YA1146) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MCM6.
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Host:
Rabbit
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Isotype:
IgG
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Application:
IHC-P
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Reactivity :
Human
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Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.189.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|
| Dilution Ratio | 1:100-1:200 |
Product Details
MCM6 Antibody (YA1146) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MCM6.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 93 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 93 kDa
Recombinant protein of human MCM6
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.189.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MCM6 acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity[1][2][3][4][5][6].
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Subcellular Localization
Nucleus; Chromosome
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Subunit
Component of the MCM2-7 complex (PubMed:16899510, PubMed:17296731, PubMed:9305914). The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (PubMed:16899510, PubMed:17296731, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:9305914). Component of the CMG helicase complex, a hexameric ring of related MCM2-7 subunits stabilized by CDC45 and the tetrameric GINS complex (PubMed:32453425, PubMed:34694004, PubMed:34700328). May interact with MCM10 (PubMed:11095689). Interacts with TIPIN (PubMed:17116885). Interacts with CDT1 (PubMed:20202939). Interacts with MCMBP (PubMed:17296731). Interacts with DDI2 (PubMed:29290612)
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SwissProt ID
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Synonyms
minichromosome maintenance complex component 6; Mis5; P105MCM; MCG40308
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Research Field
Epigenetics and Nuclear Signaling
Documentation
References
[1]. Tsuji T, et al. Essential role of phosphorylation of MCM2 by Cdc7/Dbf4 in the initiation of DNA replication in mammalian cells. Mol Biol Cell. 2006 Oct;17(10):4459-72. [Content Brief]
[2]. Rzechorzek NJ, et al. CryoEM structures of human CMG-ATPγS-DNA and CMG-AND-1 complexes. Nucleic Acids Res. 2020 Jul 9;48(12):6980-6995. [Content Brief]
[3]. Jones ML, et al. Structure of a human replisome shows the organisation and interactions of a DNA replication machine. EMBO J. 2021 Dec 1;40(23):e108819. [Content Brief]
[4]. Jenkyn-Bedford M, et al. A conserved mechanism for regulating replisome disassembly in eukaryotes. Nature. 2021 Dec;600(7890):743-747. [Content Brief]
[5]. Baris Y, et al. Fast and efficient DNA replication with purified human proteins. Nature. 2022 Jun;606(7912):204-210. [Content Brief]
[6]. Ishimi Y, et al. A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex. J Biol Chem. 1997 Sep 26;272(39):24508-13. [Content Brief]
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