METT10D Antibody (YA8533)
(Synonyms: METT10D)METT10D Antibody (YA8533) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to METT10D.
-
Host:
Mouse
-
Isotype:
IgG1
-
Application:
ICC/IF, FC
-
Reactivity :
Human
-
Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
|
|---|---|---|
| Dilution Ratio | 1:100-250 | 1:100 |
Product Details
METT10D Antibody (YA8533) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to METT10D.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman
-
Calculated Molecular Weight Predicted band size: 63.4 kDa
Full length human recombinant protein of human METT10D produced in HEK293T cell.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
-
Appearance
Solution
-
Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
METT10D is a RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure. Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, preventing recognition of their 3'-splice site by U2AF1/U2AF35, thereby inhibiting splicing and protein production of S-adenosylmethionine synthase. In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A. In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs. Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA) or 7SL RNA. Specifically binds the 3'-end of the MALAT1 long non-coding RNA[1][2][3][4][5][6][7][8].
-
Subcellular Localization
Nucleus,Cytoplasm
-
Isoforms & Post-Translational Modification
Q86W50 has two isomers: Q86W50-1: 63621 Da (predicted); Q86W50-2: 25502 Da (predicted).
-
Subunit
Interacts with MEPCE (PubMed:29051200)
-
SwissProt ID
-
Synonyms
METT10D
Documentation
Powered by Bioz
