MMACHC Antibody (YA7987)
(Synonyms: cblC)MMACHC Antibody (YA7987) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MMACHC.
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Host:
Mouse
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Isotype:
IgG1
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Application:
IHC-P, ICC/IF, FC
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Reactivity :
Human
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Formulation:
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Conjugation:
Non-conjugated
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Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:150-500 | 1:100-250 | 1:100 |
Product Details
MMACHC Antibody (YA7987) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MMACHC.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 31.5 kDa
Full length human recombinant protein of human MMACHC produced in HEK293T cell.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MMACHC is a Cobalamin (vitamin B12) cytosolic chaperone that catalyzes the reductive decyanation of cyanocob(III)alamin (cyanocobalamin, CNCbl) to yield cob(II)alamin and cyanide, using FAD or FMN as cofactors and NADPH as cosubstrate. Cyanocobalamin constitutes the inactive form of vitamin B12 introduced from the diet, and is converted into the active cofactors methylcobalamin (MeCbl) involved in methionine biosynthesis, and 5'-deoxyadenosylcobalamin (AdoCbl) involved in the TCA cycle. Forms a complex with the lysosomal transporter ABCD4 and its chaperone LMBRD1, to transport cobalamin across the lysosomal membrane into the cytosol. The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine synthase reductase) and MTR (methionine synthase) which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine. Also acts as a glutathione transferase by catalyzing the dealkylation of the alkylcob(III)alamins MeCbl and AdoCbl, using the thiolate of glutathione for nucleophilic displacement to generate cob(I)alamin and the corresponding glutathione thioether. The conversion of incoming MeCbl or AdoCbl into a common intermediate cob(I)alamin is necessary to meet the cellular needs for both cofactors. Cysteine and homocysteine cannot substitute for glutathione in this reaction[1][2][3][4][5][6][7][8][9].
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Subcellular Localization
Cytoplasm, cytosol
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Expression
Tissue_Specificity: Widely expressed. Expressed at higher level in fetal liver. Also expressed in spleen, lymph node, thymus and bone marrow. Weakly or not expressed in peripheral blood leukocytes -
Isoforms & Post-Translational Modification
Q9Y4U1: 282 amino acids, molecular weight 31728 Da.
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Subunit
Monomer in the absence of bound substrate (PubMed:21697092, PubMed:22642810)
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SwissProt ID
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Synonyms
cblC
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