MRP3 Antibody (YA3529)(PBS only)

Cat. No.: HY-P83832A: Data Sheet User Guide for Antibodies
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MRP3 Antibody (YA3529) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MRP3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MRP3 Antibody (YA3529) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MRP3.

Host

Mouse

Molecular Weight

Predicted band size: 43 kDa;

Observed band size: 170 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O15438

Gene ID

8714 [NCBI]

Immunogen

Purified recombinant fragment of human MRP3 (AA: 830-949) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：ATP-dependent transporter of the ATP-binding cassette (ABC) family that binds and hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants and endogenous compound across cell membranes (PubMed:10359813, PubMed:11581266, PubMed:15083066). Transports glucuronide conjugates such as bilirubin diglucuronide, estradiol-17-beta-o-glucuronide and GSH conjugates such as leukotriene C4 (LTC4) (PubMed:11581266, PubMed:15083066). Transports also various bile salts (taurocholate, glycocholate, taurochenodeoxycholate-3-sulfate, taurolithocholate- 3-sulfate) (By similarity). Does not contribute substantially to bile salt physiology but provides an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes (By similarity). May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (Probable). Can confer resistance to various anticancer drugs, methotrexate, tenoposide and etoposide, by decreasing accumulation of these drugs in cells (PubMed:10359813, PubMed:11581266)

Subcellular Localization：Basolateral cell membrane; Multi-pass membrane protein; Basal cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Mainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney. In testis, localized to peritubular myoid cells, Leydig cells, along the basal membrane of Sertoli cells and moderately in the adluminal compartment of the seminiferous tubules (PubMed:35307651)

Induction:Strongly up-regulated under conditions of MRP2 deficiency

Isoforms & Post-Translational Modification：O15438 has 5 isomers: O15438-1: 169343 Da (predicted); O15438-2: 137297 Da (predicted); O15438-3: 55305 Da (predicted); O15438-4: 174907 Da (predicted); O15438-5: 64632 Da (predicted).

Synonyms

MLP2; MRP3; ABC31; MOAT-D; cMOAT2; EST90757

Documentation

User Guide for Antibodies (1077 KB)