Mucin 5AC Antibody (YA4143)(PBS only)

Cat. No.: HY-P84446A: Data Sheet User Guide for Antibodies
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Mucin 5AC Antibody (YA4143) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Mucin 5AC.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Mucin 5AC Antibody (YA4143) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Mucin 5AC.

Host

Mouse

Molecular Weight

Predicted band size: 586 kDa;

Observed band size: 290 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P98088

Gene ID

4586 [NCBI]

Immunogen

Purified recombinant fragment of human MUC5AC (AA: 28-327) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed:14535999, PubMed:14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed:14535999)

Subcellular Localization：Secreted

Expression：
Tissue_specificity:It is highly expressed in respiratory tract and gastric mucosal surface cells. It is overexpressed in various cancers. It is also expressed in Barrett's esophageal epithelium and proximal duodenum.

Induction:By IL4

Subunit：Homomultimer; disulfide-linked (PubMed:14718370). The N- and C-terminus mediate their assembly into higher order structures to form filaments (By similarity). The CTCK domains of two polypeptides associate in the endoplasmic reticulum to generate intermolecularly disulfide-bonded dimers (By similarity). These dimers progress to the Golgi apparatus, which is a more acidic environment than the endoplasmic reticulum. Under acidic conditions, the N-termini form non-covalent intermolecular interactions that juxtapose assemblies from different CTCK-linked dimers to produce long, disulfide-linked polymers that remain highly compact until secretion (By similarity)

Synonyms

TBM; leB; MUC5; mucin

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/k562-cell.html [Content Brief]