NEK11 Antibody (YA8568)(PBS only)
(Synonyms: FLJ23495)NEK11 Antibody (YA8568) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NEK11.
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Host:
Mouse
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Isotype:
IgG
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Application:
FC
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
FC
FC: Flow Cytometry
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|---|---|
| Dilution Ratio | 1:100 |
Product Details
NEK11 Antibody (YA8568) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NEK11.
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Host Mouse
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 74.0 kDa
Full length human recombinant protein of human NEK11 produced in HEK293T cell.
Affinity purified
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NEK11, a serine/threonine kinase of the NIMA-related kinase family, primarily regulates cell cycle progression through the DNA damage response (DDR) [1][2]. Mechanistically, NEK11 is activated downstream of Chk1 following DNA damage, leading to phosphorylation and degradation of Cdc25A, which enforces the G2/M checkpoint[1][2]. The kinase exists as multiple isoforms, including Nek11L, Nek11S, Nek11C, and Nek11D, which exhibit distinct subcellular localization and differential impact on checkpoint control, with Nek11S showing the strongest effect[1][2]. NEK11 activity is heightened in response to genotoxic stresses such as ionizing radiation and chemotherapeutic agents, promoting cell survival by coordinating DNA repair processes[1][2][3]. In disease contexts, NEK11 overexpression is observed in colorectal and breast cancers, where its modulation influences cell proliferation and apoptosis, making it a potential therapeutic target[1][4]. Compared with related NEK isoforms, NEK11 uniquely integrates S-phase and G2/M checkpoint signaling while maintaining nucleocytoplasmic shuttling regulated by its C-terminal domain[2]. For experimental applications, NEK11 depletion via RNAi or expression of kinase-inactive mutants sensitizes cancer cells to DNA damage, enabling functional studies and preclinical exploration of targeted inhibitors[1][2][3].
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Subcellular Localization
Nucleus,Nucleus, nucleolus
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Expression
Tissue_Specificity: Poorly expressed in cerebellum, trachea, lung, appendix, and uterus -
Isoforms & Post-Translational Modification
Q8NG66 has four isomers: Q8NG66-1: 74192 Da (predicted); Q8NG66-2: 54007 Da (predicted); Q8NG66-3: 55519 Da (predicted); Q8NG66-4: 68880 Da (predicted).
Phosphorylated by NEK2 -
Subunit
Interacts with isoform 1 of NEK2
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SwissProt ID
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Synonyms
FLJ23495
Documentation
References
[2]. Noguchi K, et al. Nek11, a new member of the NIMA family of kinases, involved in DNA replication and genotoxic stress responses. J Biol Chem. 2002 Oct 18;277(42):39655-65. [Content Brief]
[3]. Gao WL, et al Integrative Analysis of the Expression Levels and Prognostic Values for NEK Family Members in Breast Cancer. Front Genet. 2022 Mar 16;13:798170. [Content Brief]
[4]. Pavan ICB, et al. On Broken Ne(c)ks and Broken DNA: The Role of Human NEKs in the DNA Damage Response. Cells. 2021 Feb 27;10(3):507. [Content Brief]
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