Oct-2 Antibody (YA5717)

Cat. No.: HY-P86025: Data Sheet User Guide for Antibodies
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Oct-2 Antibody (YA5717) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Oct-2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Oct-2 Antibody (YA5717) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Oct-2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 51 kDa;

Species Reactivity

Human, Mouse,

SwissProt ID

P09086

Gene ID

5452 [NCBI]

Immunogen

Synthesized peptide derived from human Oct-2 AA range: 250-350

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:500-5000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:2904654, PubMed:7859290). Regulates IL6 expression in B cells with POU2AF1 (By similarity). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression (PubMed:2901913, PubMed:2904654). Modulates transcription transactivation by NR3C1, AR and PGR (PubMed:10480874); Activates the U2 small nuclear RNA (snRNA) promoter

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Isomer 3 is B cell specific. Isomer 5 is expressed in B cells and the immunoglobulin-expressing T cell line MOLT-4, but not in the T cell line BW5147.

Isoforms & Post-Translational Modification：P09086 has 5 isomers: P09086-1: 51209 Da (predicted); P09086-2: 49801 Da (predicted); P09086-3: 49461 Da (predicted); P09086-4: 43528 Da (predicted); P09086-5: 62172 Da (predicted).

Subunit：Interacts with NR3C1, AR and PGR (PubMed:10480874). Interacts with POU2AF1; the interaction increases POU2F2 transactivation activity (PubMed:7859290)

Synonyms

POU domain, class 2, transcription factor 2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Octamer-binding protein 2; Oct-2; Octamer-binding transcription factor 2; OTF-2;

Documentation

User Guide for Antibodies (1077 KB)