Osteoprotegerin Antibody (YA4997)

Cat. No.: HY-P85305: Data Sheet User Guide for Antibodies
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Osteoprotegerin Antibody (YA4997) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Osteoprotegerin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Osteoprotegerin Antibody (YA4997) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Osteoprotegerin.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 46 kDa;

Observed band size: 46 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O00300

Gene ID

4982 [NCBI]

Immunogen

A synthesized peptide derived from human TNFRSF11B. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-500

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis

Subcellular Localization：Secreted

Expression：
Tissue_specificity:It is highly expressed in human lungs, heart, kidneys, liver, spleen, thymus, prostate, ovaries, small intestine, thyroid gland, lymph nodes, trachea, adrenal glands, testes, and bone marrow. Its expression level is extremely low in the brain, placenta, and skeletal muscle. It is highly expressed in fetal kidneys, liver, and lungs.

Induction:Up-regulated by increasing calcium-concentration in the medium and estrogens. Down-regulated by glucocorticoids

Subunit：Homodimer. Interacts with TNFSF10 and TNFSF11

Synonyms

TNFRSF11B; OCIF; OPG; Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin

Documentation

User Guide for Antibodies (1077 KB)