PR3 Antibody (YA5024)

Art. -Nr.: HY-P85332: Data Sheet User Guide for Antibodies
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PR3 Antibody (YA5024) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PR3.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Größe		Verfügbarkeit
50 μL		Angebot einholen
100 μL		Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

PR3 Antibody (YA5024) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PR3.

Host

Mouse

Clonality

Monoclonal

Molekulargewicht

Predicted band size: 28kDa;

Species Reactivity

Human, Mouse

SwissProt ID

P24158

Gene ID

5657 [NCBI]

Immunogen

Purified recombinant fragment of human Proteinase 3 expressed in E. Coli. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Reinheit	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) (PubMed:2033050, PubMed:28240246, PubMed:3198760). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed:23202369). Plays a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed:22266279). Triggers inflammatory processes in neutrophils by interacting with ADGRG3 upstream of F2RL1/PAR2 activation (PubMed:36302784)

Subcellular Localization：Cytoplasmic granule; Secreted; Cell membrane; Peripheral membrane protein; Extracellular side; Membrane raft; Peripheral membrane protein; Extracellular side

Expression：
Tissue_specificity:Expression at protein levels in polymorphonuclear leukocytes (PubMed:2033050, PubMed:3198760, PubMed:7897245) . Expression at protein levels in neutrophils (PubMed:17244676, PubMed:18462208, PubMed:21193407, PubMed:22266279, PubMed:28240246) . Expression in differentiating neutrophils (PubMed:18462208) .

Induction:Induced during CSF3/G-CSF-mediated neutrophil differentiation

Subunit：May form dimers (PubMed:28240246). Interacts with CD177; the interaction tethers PRTN3 to the cell surface; the interaction is direct (PubMed:17244676, PubMed:28240246). Interacts with SERPINB1 (PubMed:30692621). Interacts with ADGRG3 (PubMed:36302784)

Synonyms

PRTN3; MBN; Myeloblastin; AGP7; C-ANCA antigen; Leukocyte proteinase 3; PR-3; PR3; Neutrophil proteinase 4; NP-4; P29; Wegener autoantigen

Dokumentation

User Guide for Antibodies (1077 KB)