SLC18A2 (VMAT2) Antibody (YA7778)(PBS only)

Cat. No.: HY-P88094A: Data Sheet User Guide for Antibodies
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SLC18A2 (VMAT2) Antibody (YA7778) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SLC18A2 (VMAT2).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

SLC18A2 (VMAT2) Antibody (YA7778) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SLC18A2 (VMAT2).

Host

Mouse

Molecular Weight

Predicted band size: 55.7 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q05940

Immunogen

Full-length protein expressed in 293T cell transfected with human SLC18A2 expression vector .

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-250
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports a variety of catecholamines such as dopamine, adrenaline and noradrenaline, histamine, and indolamines such as serotonin (PubMed:23363473, PubMed:37914936, PubMed:38081299, PubMed:38517752, PubMed:8643547). Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates somatodendritic dopamine release in hippocampal neurons, likely as part of a regulated secretory pathway that integrates retrograde synaptic signals (By similarity). Acts as a primary transporter for striatal dopamine loading ensuring impulse-dependent release of dopamine at the synaptic cleft (By similarity). Responsible for histamine and serotonin storage and subsequent corelease from mast cell granules (PubMed:8860238)

Subcellular Localization：Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane,Cytoplasmic vesicle, secretory vesicle membrane,Cell projection, axon,Cell projection, dendrite

Expression：
Tissue_Specificity: Expressed in neuronal and neuroendocrine tissues. Detected in central and peripheral nervous system in particular in axonal and dendritic processes in dopaminergic cells of substantia nigra, histaminergic neuronal cell bodies of substantia nigra and tuberomammillary nucleus, in ganglion cells of sympathetic glia and in peripheral sympathetic nerve terminals in stomach and duodenum (at protein level). Highly expressed in chromaffin cells of the adrenal medulla and histamine-storing enterochromaffin-like cells of oxyntic mucosa (at protein level)

Isoforms & Post-Translational Modification：Q05940 has two isomers: Q05940-1: 55713 Da (predicted); Q05940-2: 22980 Da (predicted).

Subunit：Interacts with SLC6A3

Synonyms

SVAT; SVMT; VAT2; VMAT2

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/llc-mk2-cell.html [Content Brief]