SLC22A1/OCT1 Antibody (YA3525)(PBS only)

Art. -Nr.: HY-P83828A: Data Sheet User Guide for Antibodies
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SLC22A1/OCT1 Antibody (YA3525) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SLC22A1/OCT1.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Größe		Verfügbarkeit
50 μL		Angebot einholen
100 μL		Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung
Verweise

Beschreibung

SLC22A1/OCT1 Antibody (YA3525) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SLC22A1/OCT1.

Host

Mouse

Molekulargewicht

Predicted band size: 61 kDa;

Observed band size: 72 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O15245

Gene ID

6580 [NCBI]

Immunogen

Purified recombinant fragment of human SLC22A1 aa 284-347.

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Reinheit	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Electrogenic voltage-dependent transporter that mediates the transport of a variety of organic cations such as endogenous bioactive amines, cationic drugs and xenobiotics (PubMed:11388889, PubMed:11408531, PubMed:12439218, PubMed:12719534, PubMed:15389554, PubMed:16263091, PubMed:16272756, PubMed:16581093, PubMed:19536068, PubMed:21128598, PubMed:23680637, PubMed:24961373, PubMed:34040533, PubMed:9187257, PubMed:9260930, PubMed:9655880). Functions as a pH- and Na(+)-independent, bidirectional transporter (By similarity). Cation cellular uptake or release is driven by the electrochemical potential (i.e. membrane potential and concentration gradient) and substrate selectivity (By similarity). Hydrophobicity is a major requirement for recognition in polyvalent substrates and inhibitors (By similarity). Primarily expressed at the basolateral membrane of hepatocytes and proximal tubules and involved in the uptake and disposition of cationic compounds by hepatic and renal clearance from the blood flow (By similarity). Most likely functions as an uptake carrier in enterocytes contributing to the intestinal elimination of organic cations from the systemic circulation (PubMed:16263091). Transports endogenous monoamines such as N-1-methylnicotinamide (NMN), guanidine, histamine, neurotransmitters dopamine, serotonin and adrenaline (PubMed:12439218, PubMed:24961373, PubMed:35469921, PubMed:9260930). Also transports natural polyamines such as spermidine, agmatine and putrescine at low affinity, but relatively high turnover (PubMed:21128598). Involved in the hepatic uptake of vitamin B1/thiamine, hence regulating hepatic lipid and energy metabolism (PubMed:24961373). Mediates the bidirectional transport of acetylcholine (ACh) at the apical membrane of ciliated cell in airway epithelium, thereby playing a role in luminal release of ACh from bronchial epithelium (PubMed:15817714). Transports dopaminergic neuromodulators cyclo(his-pro) and salsolinol with lower efficency (PubMed:17460754). Also capable of transporting non-amine endogenous compounds such as prostaglandin E2 (PGE2) and prostaglandin F2-alpha (PGF2-alpha) (PubMed:11907186). May contribute to the transport of cationic compounds in testes across the blood-testis-barrier (Probable). Also involved in the uptake of xenobiotics tributylmethylammonium (TBuMA), quinidine, N-methyl-quinine (NMQ), N-methyl-quinidine (NMQD) N-(4,4-azo-n-pentyl)-quinuclidine (APQ), azidoprocainamide methoiodide (AMP), N-(4,4-azo-n-pentyl)-21-deoxyajmalinium (APDA) and 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP) (PubMed:11408531, PubMed:15389554, PubMed:35469921, PubMed:9260930); Mediates the uptake of 1-methyl-4-phenylpyridinium (MPP(+)); Not able to uptake 1-methyl-4-phenylpyridinium (MPP(+)); Not able to uptake 1-methyl-4-phenylpyridinium (MPP(+)); Not able to uptake 1-methyl-4-phenylpyridinium (MPP(+))

Subcellular Localization：Basolateral cell membrane; Multi-pass membrane protein; Apical cell membrane; Multi-pass membrane protein; Lateral cell membrane; Multi-pass membrane protein; Basal cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:It is widely expressed in the liver at high levels (PubMed:11388889, PubMed:23680637, PubMed:9187257, PubMed:9260930) . In the liver, it is mainly expressed around the central vein (PubMed:16263091) . It is also expressed in the kidneys (PubMed:9187257, PubMed:9260930) . It is also expressed in enterocytes of the small intestine (PubMed:16263091, PubMed:23680637) . In the testes, it is mainly located in peritubular myoid cells and Leydig cells, and moderately expressed in the basement membrane of Sertoli cells (PubMed:35307651) . It is also expressed in the ciliated epithelium of the trachea and bronchi in the respiratory tract (PubMed:15817714) . It is also expressed in skeletal muscle, stomach, spleen, heart, placenta, colon, brain, granulocytes, and lymphocytes (PubMed:9187257, PubMed:9260930) ; expressed in the liver and glial cell lines; expressed in the liver and glial cell lines; expressed in glial cell lines. Not expressed in the liver; expressed in glial cell lines. Not expressed in the liver.

Induction:In the liver activated by HNF4A and suppressed by bile acids via NR0B2. Increased by cholesterol treatment in hepatocyte cells

Isoforms & Post-Translational Modification：O15245 has 4 isomers: O15245-1: 61154 Da (predicted); O15245-2: 56094 Da (predicted); O15245-3: 53593 Da (predicted); O15245-4: 39224 Da (predicted).
Phosphorylated

Synonyms

OCT1; HOCT1; oct1_cds; SLC22A1

Dokumentation

User Guide for Antibodies (1077 KB)

Verweise

[1]. /biology-dictionary/cho-cell.html [Content Brief]