SPG7 Antibody (YA8277)(PBS only)

Cat. No.: HY-P88593A: Data Sheet User Guide for Antibodies
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SPG7 Antibody (YA8277) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to SPG7.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SPG7 Antibody (YA8277) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to SPG7.

Host

Mouse

Molecular Weight

Predicted band size: 88.1 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9UQ90

Immunogen

Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7 prodduced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-100
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalytic component of the m-AAA protease, a protease that plays a key role in proteostasis of inner mitochondrial membrane proteins, and which is essential for axonal and neuron development (PubMed:11549317, PubMed:28396416, PubMed:31097542, PubMed:9635427). SPG7 possesses both ATPase and protease activities: the ATPase activity is required to unfold substrates, threading them into the internal proteolytic cavity for hydrolysis into small peptide fragments (By similarity). The m-AAA protease exerts a dual role in the mitochondrial inner membrane: it mediates the processing of specific regulatory proteins and ensures protein quality control by degrading misfolded polypeptides (By similarity). Mediates protein maturation of the mitochondrial ribosomal subunit MRPL32/bL32m by catalyzing the cleavage of the presequence of MRPL32/bL32m prior to assembly into the mitochondrial ribosome (By similarity). Acts as a regulator of calcium in neurons by mediating degradation of SMDT1/EMRE before its assembly with the uniporter complex, limiting the availability of SMDT1/EMRE for MCU assembly and promoting efficient assembly of gatekeeper subunits with MCU (PubMed:28396416, PubMed:31097542). Also regulates mitochondrial calcium by catalyzing degradation of MCU (PubMed:31097542). Plays a role in the formation and regulation of the mitochondrial permeability transition pore (mPTP) and its proteolytic activity is dispensable for this function (PubMed:26387735)

Subcellular Localization：Mitochondrion inner membrane

Expression：
Tissue_Specificity: Ubiquitous

Isoforms & Post-Translational Modification：Q9UQ90 has two isomers: Q9UQ90-1: 88235 Da (predicted); Q9UQ90-2: 53940 Da (predicted).
Upon import into the mitochondrion, the N-terminal transit peptide is cleaved by the mitochondrial-processing peptidase (MPP) to generate an intermediate form which undergoes a second proteolytic cleavage mediated by proteases AFG3L2 removing an additional N-terminal fragment to generate the proteolytically active mature form

Subunit：Forms heterooligomers with AFG3L2; the m-AAA protease is composed of heterohexamers of AFG3L2 and SPG7 (PubMed:14623864, PubMed:28396416)

Synonyms

CAR; CMAR; PGN; SPG5C

Documentation

User Guide for Antibodies (1077 KB)