TCF7 Antibody (YA3979)(PBS only)

Cat. No.: HY-P84282A: Data Sheet User Guide for Antibodies
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TCF7 Antibody (YA3979) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TCF7.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TCF7 Antibody (YA3979) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TCF7.

Host

Mouse

Molecular Weight

Predicted band size: 42 kDa;

Observed band size: 45-50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P36402

Gene ID

6932 [NCBI]

Immunogen

Purified recombinant fragment of human TCF7 (AA: 168-358) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	N:A FC: 1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages (By similarity). Required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:It is primarily expressed in T cells. It can also be detected in proliferating intestinal epithelial cells and basal cells of the mammary epithelium.

Induction:By TCF7L2 and CTNNB1

Isoforms & Post-Translational Modification：P36402 has 15 isomers: P36402-5: 41552 Da (predicted); P36402-1: 41642 Da (predicted); P36402-2: 30264 Da (predicted); P36402-3: 51991 Da (predicted); P36402-9: 39681 Da (predicted); P36402-10: 28304 Da (predicted); P36402-13: 39933 Da (predicted); P36402-14: 28556 Da (predicted); P36402-15: 40418 Da (predicted); P36402-16: 29041 Da (predicted); P36402-6: 30175 Da (predicted); P36402-7: 41668 Da (predicted); P36402-8: 30291 Da (predicted); P36402-17: 40614 Da (predicted); P36402-18: 44828 Da (predicted).

Subunit：Binds the armadillo repeat of CTNNB1 and forms a stable complex (PubMed:9488439, PubMed:9783587). Interacts with TLE5, TLE1, TLE2, TLE3 and TLE4 (PubMed:11266540, PubMed:9783587). Interacts with MLLT11 (PubMed:26079538). Long isoform interacts (via N-terminus) with SOX13; inhibits WNT-mediated transcriptional activity (PubMed:17218525, PubMed:20028982). Interacts with DAZAP2 (PubMed:19304756)

Synonyms

TCF-1

Documentation

User Guide for Antibodies (1077 KB)