TLK2 Antibody (YA8128)

Cat. No.: HY-P88444: Data Sheet User Guide for Antibodies
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TLK2 Antibody (YA8128) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TLK2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TLK2 Antibody (YA8128) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TLK2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 85.3 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q86UE8

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-296 of human TLK2 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-800
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation (PubMed:10523312, PubMed:11470414, PubMed:12660173, PubMed:12955071, PubMed:29955062, PubMed:33323470, PubMed:9427565). Phosphorylates the chromatin assembly factors ASF1A and ASF1B (PubMed:11470414, PubMed:20016786, PubMed:29955062, PubMed:35136069). Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (PubMed:20016786). Negative regulator of amino acid starvation-induced autophagy (PubMed:22354037)

Subcellular Localization：Nucleus,Nucleus, nucleoplasm,Cytoplasm, perinuclear region,Cytoplasm, cytoskeleton

Expression：
Tissue_Specificity: Detected in placenta, fetal liver, kidney, pancreas, heart and skeletal muscle (PubMed:9427565). Highly expressed in testis (PubMed:9427565, PubMed:9662073). Detected in spleen, thymus, colon, ovary, small intestine, prostate and peripheral blood leukocytes (PubMed:9662073). Almost undetectable in liver and lung (PubMed:9662073)

Isoforms & Post-Translational Modification：Q86UE8 has three isomers: Q86UE8-1: 87661 Da (predicted); Q86UE8-2: 85444 Da (predicted); Q86UE8-3: 82348 Da (predicted).
Phosphorylated at Ser-750, probably by CHEK1丨Autophosphorylated; phosphorylation promotes the assembly of higher order oligomers and enzymatic activity

Subunit：Monomer (PubMed:29955062)

Synonyms

HsHPK; PKU-ALPHA

Documentation

User Guide for Antibodies (1077 KB)