FHZ
Based on 1 Customer Validation
FHZ is a fluorescent probe.
For research use only. We do not sell to patients.
- Purity: 96.21%
- CAS No.: 1883737-63-0
- Formula: C26H26N2O7
- Molecular Weight:478.49
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
After loaded with probe FHZ and treated with HClO and H2O2/EDTA-Fe2+ in order, HeLa cells display the bright fluorescences from both cyan and green channels. FHZ can give out two different fluorescent signals in the presence of both OH and HClO, suggesting the synchronous discrimination of OH and HClO by a dual-channel detection model with two exciting wavelengths. Probe FHZ shows very high specificity to the detections of OH and HClO with the excitations at 410 and 490 nm, respectively. The probe FHZ can efficiently enter the cellular mitochondria and exhibit the differentiable/visual capabilities to the endogenous OH and HClO by the dual fluorescent responses[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1883737-63-0
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Appearance Solid
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Molecular Weight 478.49
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Formula C26H26N2O7
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Color Light brown to brown
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SMILES
O=C1N(N)C2(C3=C(OC4=C2C=CC(OCCOCCOCCO)=C4)C=C(O)C=C3)C5=C1C=CC=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (208.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
HeLa cells and RAW264.7 macrophages are cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) supplied with 10% fetal bovine serum (FBS) and 1% antibotics (penicillin and streptomycin) at 37°C in humidified incubator containing 5% CO2. The cells are seeded into glass-bottomed dishes and cultured for 24 h. Subsequently, the cells are incubated with FHZ for 30 min at 37°C and then washed with PBS buffer three times. Each treatment of cells with H2O2, EDTA-Fe2+, HClO or scavengers kept 30 min[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Wild type zebrafish is used in this study. Seven-day old fertilized zebrafish embryos are cultured in 50 μM FHZ for 30 min, and then the zebrafish embryos are transferred to fresh water. A FHZ-loaded zebrafish is fixed under confocal microscope using 2% agarose gel to keep its living state for fluorescent imaging. In order to observe the release of ?OH in fresh wound, the ventral fin of the FHZ-loaded zebrafish is carefully cut a small wound using a blade. After raised for 20 min in water, the wound of injured zebrafish is imaged using confocal microscope[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0899 mL | 10.4495 mL | 20.8991 mL | 52.2477 mL |
| 5 mM | 0.4180 mL | 2.0899 mL | 4.1798 mL | 10.4495 mL | |
| 10 mM | 0.2090 mL | 1.0450 mL | 2.0899 mL | 5.2248 mL | |
| 15 mM | 0.1393 mL | 0.6966 mL | 1.3933 mL | 3.4832 mL | |
| 20 mM | 0.1045 mL | 0.5225 mL | 1.0450 mL | 2.6124 mL | |
| 25 mM | 0.0836 mL | 0.4180 mL | 0.8360 mL | 2.0899 mL | |
| 30 mM | 0.0697 mL | 0.3483 mL | 0.6966 mL | 1.7416 mL | |
| 40 mM | 0.0522 mL | 0.2612 mL | 0.5225 mL | 1.3062 mL | |
| 50 mM | 0.0418 mL | 0.2090 mL | 0.4180 mL | 1.0450 mL | |
| 60 mM | 0.0348 mL | 0.1742 mL | 0.3483 mL | 0.8708 mL | |
| 80 mM | 0.0261 mL | 0.1306 mL | 0.2612 mL | 0.6531 mL | |
| 100 mM | 0.0209 mL | 0.1045 mL | 0.2090 mL | 0.5225 mL |
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