DCA-Mln

Guidelines (The following is a recommended experimental protocol provided for guidance only; please adjust according to actual requirements)

1. Stock Solution Preparation
1.1 Solvent: MeOH–H₂O (5/5, v/v).
1.2 Concentration recommendation: 10 μM.

2. Working Solution Preparation
2.1 Diluent: MeOH–H₂O (5/5, v/v); PBS buffer; filtered natural water/methanol (5/5, v/v); ethanol with a small amount of glycerin.
2.2 Working concentration: 10 μM.
2.3 Note: Adjust working solution concentration as needed; prepare fresh before use.

3. Staining Procedure
3.1 Sample type:[1]
3.1.1 Adherent cells (MCF-7 cells).
3.2 Incubation conditions: Incubate adherent cells with 10 μM DCA-Mln for 30 min; then incubate with 0–600 μM Fe³⁺ for 30 min.
3.3 Washing steps: Wash cells twice with PBS buffer.

4. Detection & Analysis
5.1 Instrument: Fluorescence imaging system; UV-vis spectrophotometer
5.1.1 Ex = 570 nm, Em = 670 nm.
5.2 Result analysis:
5.2.1 Fluorescence intensity changes: Strong red fluorescence intensity decreases with increasing Fe³⁺ concentration; solution fluorescence changes from strong pink to non-fluorescence upon Fe³⁺ addition.
5.2.2 Color changes: Solution color changes from purple to peach pink upon Fe³⁺ addition; anti-counterfeiting ink imprints are purple in daylight and bright pink under 365 nm UV light.
5.2.3 Fluorescence localization: Red fluorescence is present in the cytoplasm of living MCF-7 cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.