Cat. No.: HY-125371

2'-C-Methyladenosine

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2'-C-Methyladenosine is an inhibitor of hepatitis C virus (HCV) replication. 2'-C-Methyladenosine inhibits HCV replicon and NS5B-catalyzed RNA synthesis with IC₅₀ values of 0.3μM and 1.9 μM, respectively. 2'-C-Methyladenosine also potently inhibits LRV1 in Leishmania guyanensis (Lgy) and Leishmania braziliensis.

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Pureté: 99.50%
CAS No.: 15397-12-3
Formule: C₁₁H₁₅N₅O₄
Masse moléculaire:281.27

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4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

2'-C-Methyladenosine Chemical Structure

CAS No.: 15397-12-3

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Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vivo
Chemical Information
Publications (3)
Solvant et solubilité
- In Vitro
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

IC50: 0.3μM (HCV replicon); 1.9 μM (NS5B)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	CC₅₀	>50 μM Compound: 2	Cytotoxicity against human A549 cells assessed as intracellular ATP level by Celltiter-Glo luminescent assay Cytotoxicity against human A549 cells assessed as intracellular ATP level by Celltiter-Glo luminescent assay	[PMID: 20457821]
A549	EC₅₀	1.12 μM Compound: 2	Antiviral activity against at 0.3 MOI Dengue virus 2 infected in human A549 cells assessed as level of E protein after 48 hrs by ELISA Antiviral activity against at 0.3 MOI Dengue virus 2 infected in human A549 cells assessed as level of E protein after 48 hrs by ELISA	[PMID: 20457821]
Caco-2	GI₅₀	>100 μM Compound: 2'-Me-Ado	Antitumor activity against human CaCo2 cells after 48 hrs by MTS assay Antitumor activity against human CaCo2 cells after 48 hrs by MTS assay	[PMID: 18588281]
HT-29	GI₅₀	>100 μM Compound: 2'-Me-Ado	Antitumor activity against human HT29 cells after 48 hrs by MTS assay Antitumor activity against human HT29 cells after 48 hrs by MTS assay	[PMID: 18588281]
HT-29	IC₅₀	>100 μM Compound: 2 (2'-Me-Ado)	In vitro inhibitory activity against human colon carcinoma HT-29 cell line In vitro inhibitory activity against human colon carcinoma HT-29 cell line	[PMID: 16033277]
Huh-5-2	CC₅₀	>33 μM Compound: 2'-C-Methyladenosine	Cytotoxicity against human Huh5-2 cells after 3 days by MTT assay Cytotoxicity against human Huh5-2 cells after 3 days by MTT assay	[PMID: 18625766]
Huh-5-2	CC₅₀	>50 μM Compound: 1	Cytotoxicity against human Huh5-2 cells Cytotoxicity against human Huh5-2 cells	[PMID: 19523820]
Huh-5-2	EC₅₀	0.12 μM Compound: 2'-C-Methyladenosine	Antiviral activity against Hepatitis C virus genotype 1b infected in human Huh5-2 cells assessed as reduction in replicon RNA after 4 days by luciferase assay Antiviral activity against Hepatitis C virus genotype 1b infected in human Huh5-2 cells assessed as reduction in replicon RNA after 4 days by luciferase assay	[PMID: 18625766]
Huh-5-2	EC₅₀	0.25 μM Compound: 1	Antiviral activity against HCV assessed as inhibition of viral replicon replication in human Huh5-2 cells Antiviral activity against HCV assessed as inhibition of viral replicon replication in human Huh5-2 cells	[PMID: 19523820]
Huh-7	CC₅₀	>100 μM Compound: 1	Cytotoxicity against Huh7 cells after 24 hrs by MTS assay Cytotoxicity against Huh7 cells after 24 hrs by MTS assay	[PMID: 17521911]
Huh-7	CC₅₀	>100 μM Compound: 2'CMeA	Cytotoxicity against human Huh7 cells harboring con1 replicon after 3 days by MTT assay Cytotoxicity against human Huh7 cells harboring con1 replicon after 3 days by MTT assay	[PMID: 17997319]
Huh-7	CC₅₀	>100 μM Compound: 2'CmeA	Cytotoxicity against human Huh7 cells after 3 days by MTT method Cytotoxicity against human Huh7 cells after 3 days by MTT method	[PMID: 18078757]
Huh-7	CC₅₀	15 μM Compound: 2	Cytotoxicity against Huh7 cells by MTS assay Cytotoxicity against Huh7 cells by MTS assay	[PMID: 16368235]
Huh-7	CC₅₀	55 μM Compound: 2 mA	Cytotoxicity against human HuH7 cells infected with HCV1b after 3 days by MTS assay Cytotoxicity against human HuH7 cells infected with HCV1b after 3 days by MTS assay	[PMID: 22100256]
Huh-7	CC₅₀	66 μM Compound: 2'-C-Me A	Cytotoxicity against human HuH7 cells after 24 hrs by MTS assay Cytotoxicity against human HuH7 cells after 24 hrs by MTS assay	[PMID: 22578461]
Huh-7	EC₅₀	>100 μM Compound: 2	Antiviral activity against HCV genotype1 infected in human Huh7 cells assessed as reduction of viral replication by steady-Glo luciferase assay Antiviral activity against HCV genotype1 infected in human Huh7 cells assessed as reduction of viral replication by steady-Glo luciferase assay	[PMID: 20627592]
Huh-7	EC₅₀	0.2 μM Compound: 1	Antiviral activity against HCV genotype 1b in Huh7 cells assessed as inhibition of viral RNA replication after 72 hrs by ribonuclease protection assay Antiviral activity against HCV genotype 1b in Huh7 cells assessed as inhibition of viral RNA replication after 72 hrs by ribonuclease protection assay	[PMID: 17521911]
Huh-7	EC₅₀	0.2 μM Compound: 2 mA	Antiviral activity against HCV genotype 1b infected in human HuH7 cells after 3 days by renilla luciferase reporter assay Antiviral activity against HCV genotype 1b infected in human HuH7 cells after 3 days by renilla luciferase reporter assay	[PMID: 22100256]
Huh-7	EC₅₀	0.23 μM Compound: 2'CMeA	Antiviral activity against HCV 1b Con1 replicon in Huh7 cells Antiviral activity against HCV 1b Con1 replicon in Huh7 cells	[PMID: 17997319]
Huh-7	EC₅₀	0.25 μM Compound: 1	Antiviral activity against HCV genotype 1b in Huh7 cells assessed as inhibition of viral RNA replication after 24 hrs by ribonuclease protection assay Antiviral activity against HCV genotype 1b in Huh7 cells assessed as inhibition of viral RNA replication after 24 hrs by ribonuclease protection assay	[PMID: 17521911]
Huh-7	EC₅₀	0.35 μM Compound: 2'-C-Me A	Antiviral activity against HCV genotype 1b infected in human HuH7 cells assessed as cytoprotection after 24 hrs Antiviral activity against HCV genotype 1b infected in human HuH7 cells assessed as cytoprotection after 24 hrs	[PMID: 22578461]
K562	GI₅₀	16 μM Compound: 2'-Me-Ado	Antitumor activity against human K562 cells after 48 hrs by MTS assay Antitumor activity against human K562 cells after 48 hrs by MTS assay	[PMID: 18588281]
K562	IC₅₀	>100 μM Compound: 2 (2'-Me-Ado)	In vitro inhibitory activity against human leukemia K562IU cell line In vitro inhibitory activity against human leukemia K562IU cell line	[PMID: 16033277]
K562	IC₅₀	16 μM Compound: 2 (2'-Me-Ado)	In vitro inhibitory activity against human myelogenous leukemia K562 cell line In vitro inhibitory activity against human myelogenous leukemia K562 cell line	[PMID: 16033277]
MCF7	GI₅₀	>100 μM Compound: 2'-Me-Ado	Antitumor activity against human MCF7 cells after 48 hrs by MTS assay Antitumor activity against human MCF7 cells after 48 hrs by MTS assay	[PMID: 18588281]
MCF7	IC₅₀	>100 μM Compound: 2 (2'-Me-Ado)	In vitro inhibitory activity against human breast carcinoma MCF-7 cell line In vitro inhibitory activity against human breast carcinoma MCF-7 cell line	[PMID: 16033277]
MDBK441	CC₅₀	>14 μg/mL Compound: 2-C-methyl adenosine	Cytotoxicity against MDBK cells Cytotoxicity against MDBK cells	[PMID: 16610805]

In Vivo

2'-C-Methyladenosine has inhibitory potency for HCV replicon in HB110A cells with an IC₅₀ values of 0.3μM^[1].
? 2'-C-Methyladenosine inhibits NS5B-catalyzed RNA synthesis with an IC₅₀ values of 1.9 μM^[1].
? 2′-C-methyladenosine potently inhibits LRV1 in Leishmania guyanensis (Lgy) and Leishmania braziliensis^[2]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 15397-12-3
Appearance Solid
Masse moléculaire 281.27
Formule C₁₁H₁₅N₅O₄
Color White to off-white
SMILES

OC[C@@H]1[C@@H](O)[C@@](C)(O)[C@H](N2C3=NC=NC(N)=C3N=C2)O1
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

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Algal Res

2024 April.
iScience

Mitochondrial dysfunction activates ADAMTS-5 expression via mt-dsRNA-PKR-Spi-1 axis in osteoarthritic chondrocytes. [Abstract]

2026 May 20;29(6):115980. PMID: 42211122
bioRxiv

2024 Jan 12.

Solvant et solubilité

In Vitro:

DMSO : 31.25 mg/mL (111.10 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

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Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5553 mL

17.7765 mL

35.5530 mL

5 mM

0.7111 mL

3.5553 mL

7.1106 mL

10 mM

0.3555 mL

1.7777 mL

3.5553 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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Molecular Weight Molecular Weight

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Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (278 KB)
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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Steven S Carroll, et al. Inhibition of hepatitis C virus RNA replication by 2'-modified nucleoside analogs. J Biol Chem. 2003 Apr 4;278(14):11979-84. [Content Brief]

[2]. John I Robinson, et al. Concentration of 2'C-methyladenosine triphosphate by Leishmania guyanensis enables specific inhibition of Leishmania RNA virus 1 via its RNA polymerase. J Biol Chem. 2018 Apr 27;293(17):6460-6469. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5553 mL	17.7765 mL	35.5530 mL	88.8826 mL
	5 mM	0.7111 mL	3.5553 mL	7.1106 mL	17.7765 mL
	10 mM	0.3555 mL	1.7777 mL	3.5553 mL	8.8883 mL
	15 mM	0.2370 mL	1.1851 mL	2.3702 mL	5.9255 mL
	20 mM	0.1778 mL	0.8888 mL	1.7777 mL	4.4441 mL
	25 mM	0.1422 mL	0.7111 mL	1.4221 mL	3.5553 mL
	30 mM	0.1185 mL	0.5926 mL	1.1851 mL	2.9628 mL
	40 mM	0.0889 mL	0.4444 mL	0.8888 mL	2.2221 mL
	50 mM	0.0711 mL	0.3555 mL	0.7111 mL	1.7777 mL
	60 mM	0.0593 mL	0.2963 mL	0.5926 mL	1.4814 mL
	80 mM	0.0444 mL	0.2222 mL	0.4444 mL	1.1110 mL
	100 mM	0.0356 mL	0.1778 mL	0.3555 mL	0.8888 mL

Keywords

2'-C-Methyladenosine

3 Cited Publications

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2'-C-Methyladenosine

Anti-Infection Compound Library

Immunology/Inflammation Compound Library

Antiviral Compound Library

Nucleotide Compound Library

Anti-Hepatitis C Virus Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

MCE Kinase Hinge Binder Fragment Library

Publications Citing Use of MedChemExpress (MCE) 2'-C-Methyladenosine

Activité biologique

Chemical Information

Publications (3)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

2'-C-Methyladenosine

Anti-Infection Compound Library

Immunology/Inflammation Compound Library

Antiviral Compound Library

Nucleotide Compound Library

Anti-Hepatitis C Virus Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

MCE Kinase Hinge Binder Fragment Library

Publications Citing Use of MedChemExpress (MCE) 2'-C-Methyladenosine

Activité biologique

Chemical Information

Publications (3)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

2'-C-Methyladenosine Related Classifications

HY-125371 Related Classifications