Fibronectin Adhesion-promoting Peptide
Based on 1 Customer Validation
Fibronectin Adhesion-promoting Peptide (Heparin Binding Peptide) is one of the heparin-binding amino acid sequences found in the carboxy-terminal heparin-binding domain of fibronectin. It promotes assembly of mesenchymal stem cell (MSC) spheroids into larger aggregates. Fibronectin Adhesion-promoting Peptide directly promotes the adhesion, spreading, and migration of endothelial cells by reacting with heparin binding domains of cells.
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- Pureté : 98.50%
- CAS No.: 125720-21-0
- Formule: C47H74N16O10
- Masse moléculaire:1023.19
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Stockage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
GRGDS and Fibronectin Adhesion-promoting Peptide (Heparin Binding Peptide: WQPPRARI) micropatterns increase the endothelialisation of prosthetic materials in vitro. Fibronectin Adhesion-promoting Peptide also enhances haptotactic cell migration. The anti-apoptotic effects of fibronectin require the presence of the WQPPRARI sequence, which may be due to synergistic survival signals mediated by this sequence and by the RGD motif[1].
RDGS and Fibronectin Adhesion-promoting Peptide promote cell adhesion through different activation pathways that induce different forms of adhesion which can be highlighted through the analysis of focal and fibrillar contacts[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 125720-21-0
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Appearance Solid
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Masse moléculaire 1023.19
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Formule C47H74N16O10
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Color White to off-white
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Synonyms
Heparin Binding Peptide
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Sequence
Trp-Gln-Pro-Pro-Arg-Ala-Arg-Ile
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Sequence Shortening
WQPPRARI
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
In Vitro:
DMSO : ≥ 50 mg/mL (48.87 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 2 mg/mL (1.95 mM; Need ultrasonic and warming)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 2 mg/mL (1.95 mM); Clear solution; Need ultrasonic
Protocole
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Cell migration
Cell migration is a method that plays an important role in wound healing, cell differentiation, embryonic development, etc.
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Endothelial Tube Formation Assay
Endothelial tube formation assay evaluates the ability of endothelial cells to attach, migrate, align, and organize into capillary-like networks when cultured on gelled basement membrane extract or Matrigel; the readout is the morphology and quantity of tube-like networks, which reflects an in vitro endothelial morphogenesis step related to angiogenesis. Basement membrane extract/Matrigel provides laminin-rich extracellular matrix cues that support endothelial differentiation into capillary-like structures, but it can contain biologically active growth factors, so growth-factor-reduced matrix is preferred when testing defined angiogenic stimulators or inhibitors.
Pureté et documentation
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Fiche technique (302 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Hoesli CA, et al. A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces. Biomaterials. 2014 Jan;35(3):879-90. [Content Brief]
[2]. Chollet C, et al. Impact of peptide micropatterning on endothelial cell actin remodeling for cell alignment under shear stress. Macromol Biosci. 2012 Dec;12(12):1648-59. [Content Brief]
[3]. Lei J, Murphy WL, et al. Combination of Heparin Binding Peptide and Heparin Cell Surface Coatings for Mesenchymal Stem Cell Spheroid Assembly. Bioconjug Chem. 2018 Apr 18;29(4):878-884. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 0.9773 mL | 4.8867 mL | 9.7734 mL | 24.4334 mL |
| DMSO | 5 mM | 0.1955 mL | 0.9773 mL | 1.9547 mL | 4.8867 mL |
| 10 mM | 0.0977 mL | 0.4887 mL | 0.9773 mL | 2.4433 mL | |
| 15 mM | 0.0652 mL | 0.3258 mL | 0.6516 mL | 1.6289 mL | |
| 20 mM | 0.0489 mL | 0.2443 mL | 0.4887 mL | 1.2217 mL | |
| 25 mM | 0.0391 mL | 0.1955 mL | 0.3909 mL | 0.9773 mL | |
| 30 mM | 0.0326 mL | 0.1629 mL | 0.3258 mL | 0.8144 mL | |
| 40 mM | 0.0244 mL | 0.1222 mL | 0.2443 mL | 0.6108 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Fibronectin Adhesion-promoting Peptide Related Classifications
HY-P0306 Related Classifications
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