BDL-E5

Guide (The following is our recommended protocol. This protocol is for guidance only and should be modified according to your specific needs).

1. Stock Solution Preparation
1.2 Concentration recommendation: 500 nM

3. Staining Procedure
3.1 Sample type:[1]
3.1.1 Adherent cells: Trypsinization is needed for harvest prior to FACS.
3.1.2 Sample types include human adipose-derived stromal cells (ASCs), dental pulp stem cells (DPSCs), human-induced pluripotent stem (iPS) cells, and reprogramming somatic cells
3.2 Incubation conditions: Incubate with dye solution for 1 h.
3.3 Washing steps: No washing required for imaging; wash once with PBS for specific post-staining imaging conditions.

4. Controls
4.1 Non-reprogrammed somatic cells (ASCs, DPSCs) serve as negative controls.
4.2 Mature human iPS cells serve as positive controls.
4.3 Unstained cells serve as blank controls for FACS.

5. Detection & Analysis
5.1 Instrument: High-content imaging system or fluorescence-activated cell sorter (FACS)
5.1.1 Ex/Em wavelength: 578 nm/599 nm
5.2 Result analysis:
5.2.1 Fluorescence intensity changes: Increased fluorescence intensity correlates with progression of reprogramming, with positive cells showing significantly higher intensity than non-reprogrammed somatic cells.
5.2.2 Fluorescence localization: Co-localizes with Golgi complex marker in reprogramming cells.
5.2.3 Color changes: Yellow fluorescence is emitted from positive cells.
5.2.4 BDL-E5-positive cells are positive for pluripotency marker TRA-1-60, and give rise to viable iPS colonies upon sorting.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.