Kirrel1/NEPH1 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
The Kirrel1/NEPH1 protein is essential for the normal function of the glomerular filtration barrier, helping to maintain a stable podocyte structure and interdigitated foot processes connected by specialized cell-to-cell junctions called slit septa. Additionally, it acts as a signaling protein that requires the presence of TEC kinase to fully transactivate the transcription factor AP-1. Kirrel1/NEPH1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kirrel1/NEPH1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Mouse
- Source: HEK293
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Stockage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Activité biologique
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Activité biologique
Description
The Kirrel1/NEPH1 protein is essential for the normal function of the glomerular filtration barrier, helping to maintain a stable podocyte structure and interdigitated foot processes connected by specialized cell-to-cell junctions called slit septa. Additionally, it acts as a signaling protein that requires the presence of TEC kinase to fully transactivate the transcription factor AP-1. Kirrel1/NEPH1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kirrel1/NEPH1 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
Kirrel1, also known as NEPH1, is indispensable for maintaining the proper function of the glomerular filtration barrier. It plays a crucial role in preserving a stable podocyte architecture, characterized by interdigitating foot processes connected through specialized cell-cell junctions known as the slit diaphragm. As a signaling protein, Kirrel1 requires the presence of TEC kinases for full trans-activation of the transcription factor AP-1. It engages in interactions with key proteins such as TJP1/ZO-1 and NPHS2/podocin, contributing to the intricate molecular network governing podocyte structure and function. Additionally, Kirrel1 forms homodimers through its Ig-like domains and, when tyrosine-phosphorylated, interacts with the signaling adaptor protein GRB2. Notably, its interaction with NPHS1/nephrin relies on Kirrel1 glycosylation, highlighting the complexity of its regulatory roles in podocyte biology.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-6*His
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Accession
Q80W68-1 (L48-L525)
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Molecular Construction
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N-term
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Kirrel1 (L48-L525)
Accession # Q80W68-1 -
6*His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
KIRREL1; Kin Of Irregular Chiasm-Like Protein 1; Prev. KIRREL; Kin Of IRRE Like (Drosophila); NEPH1; Kin Of IRRE Like; Kin Of IRRE-Like Protein 1; Nephrin Related; Nephrin-Like Protein 1; NPHS23; Kirre Like Nephrin Family Adhesion Molecule 1; CDNA FLJ5636
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AA Sequence
LPGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEETRIDGGPVILLQAGTPYNLTCRAFNAKPAATIIWFRDGTQQEGAVTSTELLKDGKRETTISQLLIEPTDLDIGRVFTCRSMNEAIPNGKETSIELDVHHPPTVTLSIEPQTVLEGERVIFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVYNKVGSTNVSTLVNVHFAPRIVVYPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQFAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVL
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Predicted Molecular Mass
53.4 kDa
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Masse moléculaire
Approximately 70-85 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Pureté
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, 1 mM EDTA, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Fiche technique (262 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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