Carboxypeptidase B (MS grade)
Based on 1 Customer Validation
Carboxypeptidase B (MS grade) is a peptide exonuclease that can specifically degrade peptide chains. Carboxypeptidase B (MS grade) is progressively degraded from the C-terminal to release free amino acids. Carboxypeptidase B (MS grade) hydrolyzes only peptide bonds with basic amino acids (such as arginine and lysine) as C-terminal residues.
For research use only. We do not sell to patients.
- CAS No.: 9025-24-5
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
This product can be used for: 1) detection of antibody basic peaks; 2) determination of protein C-terminal amino acids.
Protocol
1) Dissolution: Dissolve carboxypeptidase B in pure water or 25mM Tris-HCl (pH 7.5-8.0) at a final concentration of 1-10mg/ml.
2) Enzyme digestion: The recommended ratio of carboxypeptidase B and target protein is 1:20-1:100 (w:w) for enzyme digestion, and the optimal enzyme digestion pH is 7.0-9.0.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.4.17.2
≥200 U/mg protein
One unit is defined as the amount of enzyme that can catalyze the Hippuryl-Arg of 1μmol to hydrolysis in 1 min at 25°C and pH 7.6
Chemical Information
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CAS No. 9025-24-5
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Appearance Solid
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Color White to off-white
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SMILES
[Carboxypeptidase B (MS grade)]
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Synonyms
EC 3.4.2.2 (MS grade)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (265 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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Handling Instructions (2659 KB)
References
[1]. FOLK JE, et al. The kinetics of carboxypeptidase B activity. II. Kinetic parameters of the cobalt and cadmium enzymes. J Biol Chem. 1962 Oct;237:3100-4. [Content Brief]
[2]. Qian X, et al. Molecular characterization of carboxypeptidase B-like (CPB) in Scylla paramamosain and its role in white spot syndrome virus and Vibrio alginolyticus infection. Fish Shellfish Immunol. 2019 Nov;94:434-446. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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