Pepsin (MS Grade)
Based on 3 publication(s) in Google Scholar
Pepsin (MS Grade) is the major pig and human gastric proteases, it is a pepsin-like minor gastric proteolytic enzymes. Pepsin (MS Grade) contributes to proteolysis of food proteins in the vertebrate stomach.
For research use only. We do not sell to patients.
- CAS No.: 9001-75-6
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Pepsin (MS Grade)
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
This product can be used for: 1) protein analysis in mass spectrometry and other applications; 2) for structural protein research (HDX exchange) and antibody analysis; 3) for digestion of tightly folded proteins in proteomics; 4) digestion of casein for vaccine production protein.
Protocol
1) Dissolve the target protein: Dissolve the protein in pH 1.0-5.0 buffer;
2) Enzyme dissolution: Dissolve pepsin powder in double-distilled water. Note that the pH should be lower than 5.5 so that the final concentration of pepsin is 1 mg/mL;
3) Enzyme digestion: Add pepsin to the target protein solution. The recommended ratio is enzyme: protein = 1:20-1:100 (W: W). After mixing, react at 37°C for 1-18 hours and heat at 95°C. Stop the reaction after 10 minutes.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 9001-75-6
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Appearance Solid
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Color Off-white to light yellow
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SMILES
[Pepsin (MS Grade)]
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Synonyms
EC 3.4.23.1 (MS Grade)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (3)
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Journal Impact Factor
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Most Recent
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Transl Neurodegener
Bacteroides coprocola protects dopaminergic neurons in rotenone-induced Parkinson's disease mouse model by modulating gut microbiota dysbiosis and inhibiting the NLRP3 signaling pathway. [Abstract]2026 Feb 28;15(1):7. PMID: 41761283 -
Autophagy
2025 Oct 14:1-23. PMID: 41037659 -
Purity & Documentation
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Data Sheet (268 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Portuguese - PT (557 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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