Cat. No.: HY-125859

Peroxidase, Horseradish

2 Cited Publications

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Based on 2 publication(s) in Google Scholar

Peroxidase, Horseradish actively involves in oxidizing reactive oxygen species, innate immunity, hormone biosynthesis and pathogenesis of several diseases.

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CAS No.: 9003-99-0

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Please store the product under the recommended conditions in the Certificate of Analysis.

Peroxidase, Horseradish Chemical Structure

CAS No.: 9003-99-0

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Peroxidase, Horseradish actively involves in oxidizing reactive oxygen species, innate immunity, hormone biosynthesis and pathogenesis of several diseases^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.9 μM Compound: 8, MGCD0103	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 18570366]
A549	IC₅₀	1.279 μM Compound: MGCD0103	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 23206867]
A549	IC₅₀	1.65 μM Compound: MGCD0103	Antiproliferative activity against human A549 cells assessed as cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24095018]
A549	IC₅₀	1.73 μM Compound: MGCD0103, Mocetinostat	Cytotoxicity against human A549 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay Cytotoxicity against human A549 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay	[PMID: 23829483]
A549	IC₅₀	11.87 μM Compound: MGCD0103	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay	10.1039/C5MD00247H
A549	IC₅₀	14.57 μM Compound: MGCD0103	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	10.1039/C5MD00247H
A549	IC₅₀	2.08 μM Compound: Mocetinostat	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
A549	IC₅₀	59.9 μM Compound: MGCD0103	Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 24 hrs by MTT assay	10.1039/C5MD00247H
CT26	GI₅₀	1.77 μM Compound: 4; MGCD-0103	Antiproliferative activity against mouse CT26 cells after 72 hrs by CCK-8 assay Antiproliferative activity against mouse CT26 cells after 72 hrs by CCK-8 assay	[PMID: 35390714]
CT26	GI₅₀	1.8 μM Compound: 9; MGCD-0103	Antiproliferative activity against mouse CT26 cells measured after 72 hrs by CCK8 assay Antiproliferative activity against mouse CT26 cells measured after 72 hrs by CCK8 assay	[PMID: 34783558]
DU-145	IC₅₀	0.67 μM Compound: 8, MGCD0103	Antiproliferative activity against human Du145 cells after 72 hrs by MTT assay Antiproliferative activity against human Du145 cells after 72 hrs by MTT assay	[PMID: 18570366]
DU-145	IC₅₀	2.06 μM Compound: MGCD0103, Mocetinostat	Cytotoxicity against human DU145 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay Cytotoxicity against human DU145 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay	[PMID: 23829483]
HCT-116	EC₅₀	0.6 μM Compound: A, MGCD0103	Induction of p21WAF1/CIP1 expression in human HCT116 cells assessed as tubulin level after 16 hrs by luciferase assay Induction of p21WAF1/CIP1 expression in human HCT116 cells assessed as tubulin level after 16 hrs by luciferase assay	[PMID: 19114304]
HCT-116	IC₅₀	0.29 μM Compound: 8, MGCD0103	Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 18570366]
HCT-116	IC₅₀	0.3 μM Compound: A, MGCD0103	Antiproliferative activity against human HCT116 cells by MTT assay Antiproliferative activity against human HCT116 cells by MTT assay	[PMID: 19114304]
HCT-116	IC₅₀	0.327 μM Compound: MGCD0103	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 23206867]
HCT-116	IC₅₀	0.396 μM Compound: Mocetinostat	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
HCT-116	IC₅₀	0.7 μM Compound: MGCD0103, Mocetinostat	Cytotoxicity against human HCT116 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay Cytotoxicity against human HCT116 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay	[PMID: 23829483]
HCT-116	IC₅₀	1.24 μM Compound: MGCD0103	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 48 hrs by MTT assay	10.1039/C5MD00247H
HCT-116	IC₅₀	1.57 μM Compound: MGCD0103	Antiproliferative activity against human HCT116 cells assessed as cell viability after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24095018]
HCT-116	IC₅₀	29.69 μM Compound: MGCD0103	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 24 hrs by MTT assay	10.1039/C5MD00247H
HCT-116	IC₅₀	3.51 μM Compound: MGCD0103	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 72 hrs by MTT assay	10.1039/C5MD00247H
HeLa	IC₅₀	3.32 μM Compound: MGCD0103	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay	10.1039/C5MD00247H
HeLa	IC₅₀	3.42 μM Compound: MGCD0103	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay	10.1039/C5MD00247H
HeLa	IC₅₀	43.8 μM Compound: MGCD0103	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 24 hrs by MTT assay	10.1039/C5MD00247H
Hep 3B2	IC₅₀	0.823 μM Compound: Mocetinostat	Cytotoxicity against human Hep3B cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human Hep3B cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
HepG2	IC₅₀	0.876 μM Compound: Mocetinostat	Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
HepG2	IC₅₀	4.05 μM Compound: MGCD0103	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay	10.1039/C5MD00247H
HepG2	IC₅₀	4.25 μM Compound: MGCD0103	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	10.1039/C5MD00247H
HepG2	IC₅₀	5.79 μM Compound: MGCD0103	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTT assay	10.1039/C5MD00247H
HMEC	IC₅₀	20 μM Compound: 8, MGCD0103	Antiproliferative activity against HMEC after 72 hrs by MTT assay Antiproliferative activity against HMEC after 72 hrs by MTT assay	[PMID: 18570366]
HMEC	IC₅₀	21 μM Compound: A, MGCD0103	Antiproliferative activity against HMEC by MTT assay Antiproliferative activity against HMEC by MTT assay	[PMID: 19114304]
MC-38	GI₅₀	1.64 μM Compound: 4; MGCD-0103	Antiproliferative activity against mouse MC38 cells after 72 hrs by CCK-8 assay Antiproliferative activity against mouse MC38 cells after 72 hrs by CCK-8 assay	[PMID: 35390714]
MC-38	GI₅₀	21 μM Compound: 9; MGCD-0103	Antiproliferative activity against mouse MC38 cells measured after 72 hrs by CCK8 assay Antiproliferative activity against mouse MC38 cells measured after 72 hrs by CCK8 assay	[PMID: 34783558]
MCF7	IC₅₀	1.26 μM Compound: MGCD0103, Mocetinostat	Cytotoxicity against human MCF7 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay Cytotoxicity against human MCF7 cells assessed as growth inhibition by measuring cellular ATP level after 72 hrs by cell-titer glo assay	[PMID: 23829483]
MCF7	IC₅₀	2.49 μM Compound: MGCD0103	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	10.1039/C5MD00247H
MCF7	IC₅₀	4.807 μM Compound: MGCD0103	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 23206867]
MCF7	IC₅₀	56.81 μM Compound: MGCD0103	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay	10.1039/C5MD00247H
MCF7	IC₅₀	84.17 μM Compound: MGCD0103	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 24 hrs by MTT assay	10.1039/C5MD00247H
MHCC97H	IC₅₀	4.563 μM Compound: Mocetinostat	Cytotoxicity against human MHCC97H cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MHCC97H cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
MKN-45	IC₅₀	0.61 μM Compound: Mocetinostat	Cytotoxicity against human MKN45 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MKN45 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
PANC-1	IC₅₀	26.774 μM Compound: Mocetinostat	Cytotoxicity against human PANC1 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human PANC1 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
Sf9	IC₅₀	>10000 nM Compound: MGCD0103	Inhibition of human recombinant HDAC6 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate Inhibition of human recombinant HDAC6 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate	[PMID: 23009203]
Sf9	IC₅₀	102 nM Compound: MGCD0103	Inhibition of human recombinant HDAC1 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate Inhibition of human recombinant HDAC1 expressed in Sf9 cells incubated for 2 hrs using RHKK-Ac fluorogenic substrate	[PMID: 23009203]
SJSA-1	IC₅₀	3.624 μM Compound: Mocetinostat	Cytotoxicity against human SJSA1 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human SJSA1 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
SNU-16	IC₅₀	0.142 μM Compound: Mocetinostat	Cytotoxicity against human SNU16 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human SNU16 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
SNU-5	IC₅₀	1.009 μM Compound: Mocetinostat	Cytotoxicity against human SNU5 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human SNU5 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
SW-620	IC₅₀	0.419 μM Compound: Mocetinostat	Cytotoxicity against human SW620 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human SW620 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25805446]
T-24	EC₅₀	<1 μM Compound: A, MGCD0103	Induction of histone H4 hyperacetylation in human T24 cells after 16 hrs by immunoblotting Induction of histone H4 hyperacetylation in human T24 cells after 16 hrs by immunoblotting	[PMID: 19114304]

In Vitro

Peroxidases belong to a large family of isoenzymes present in almost all living organisms. These are generally heme containing enzymes ranging in Mw from 35-100 Kd. Mammalian peroxidases are much larger proteins (576-738 amino acids) than the plant counterparts. Peroxidases exist as monomers, dimmers or tetramers and their gene locations also vary among different chromosomes. Peroxidases have some organ, tissue, cellular and sub-cellular specific distribution patterns, performing some specific functions^[1]. Product Information
Optimal pH: 6.0 - 7.0
Theoretical molecular weight: 44 kDa

Instructions
Dissolved in pure water (1 mg/mL). It is recommended to use freshly prepared products. Or it can be dissolved according to the buffer in the specific experimental reference (such as 0.1 M phosphate buffer, pH 6.0).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EC Number

1.11.1.7

Enzyme Activity

≥150 U/mg soild

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the production of 1 mg purpurogallin in per 20s at 20°C, pH 6.0.

Chemical Information

CAS No. 9003-99-0
Appearance Solid
Color Brown to reddish brown
SMILES

[Peroxidase, Horseradish]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (2)

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Journal Impact Factor

Adv Sci (Weinh)

Rational Design of Natural Xanthones Against Gram-negative Bacteria. [Abstract]

2025 Apr;12(14):e2411923. PMID: 39888292
Cell Rep

Mrgpra2+ neutrophils integrate infection-derived signals to trigger NET-mediated antimicrobial defense in bone marrow. [Abstract]

2026 Jun 22:117579. PMID: 42330955

Solvent & Solubility

In Vitro:

H₂O : 33.33 mg/mL (ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 10 mg/mL; Clear solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 0.1M Phosphate Buffer

Solubility: 2 mg/mL; Clear solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Khan AA, et al. Biochemical and pathological studies on peroxidases -an updated review. Glob J Health Sci. 2014 May 13;6(5):87-98. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Peroxidase, Horseradish

2 Cited Publications

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Peroxidase, Horseradish

Publications Citing Use of MedChemExpress (MCE) Peroxidase, Horseradish

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Calculators

Help & FAQs

Peroxidase, Horseradish Related Classifications

HY-125859 Related Classifications