DBCO-PEG4-TAMRA
Based on 1 Customer Validation
DBCO-PEG4-TAMRA is a PEG-based TAMRA dye and contains a DBCO group, which enables Click Chemistry. The TAMRA dye is a dye widely used in oligonucleotide labeling and automated DNA sequencing applications. DBCO-PEG4-TAMRA is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 96.21%
- CAS. Nr.: 1895849-41-8
- Formel: C54H57N5O10
- Molecular Weight:936.06
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Speicherung:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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HY-15943
6-TAMRAFluorescent Dye -
HY-123629
TAMRA-PEG3-AzideDye -
HY-120666
TAMRA-PEG4-AlkyneDye -
HY-140272
DBCO-PEG4-NHS esterPROTAC Linker -
HY-120770
DBCO-PEG4-MaleimideADC Linker -
HY-140274
DBCO-PEG12-NHS esterPROTAC Linker -
HY-126885
DBCO-PEG5-NHS esterADC/PROTAC Linker -
HY-130396
DBCO-PEG4-C2-acidPROTAC Linker -
HY-141286
TAMRA-PEG4-methyltetrazineDye -
HY-134714
DBCO-PEG3-aminePROTAC Linker -
HY-140301
DBCO-PEG4-DesthiobiotinPROTAC Linker -
HY-151822
Hydroxy-PEG3-DBCOAzide Compound
Biologische Aktivität
Beschreibung
IC50 & Target
TARMA Dye
In Vitro
Click chemistry refers to a group of reactions that are fast, simple to use, easy to purify, versatile, regiospecific, and give high product yields. Click chemistry reaction is suitable for cell marker and low concentration reaction with low toxicity.DBCO reacts with azide functionalized compounds or biomolecules without catalyst to form a stable triazole linkage, which is an ideal alternative to copper intolerant applications.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS. Nr. 1895849-41-8
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Appearance Solid
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Molecular Weight 936.06
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Formel C54H57N5O10
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Color Light brown to brown
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SMILES
CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(C(NCCOCCOCCOCCOCCC(NCCC(N5C6=CC=CC=C6C#CC7=CC=CC=C7C5)=O)=O)=O)C=C4C([O-])=O)=C2C=C1)C
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 50 mg/mL (53.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Reinheit & Dokumentation
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Data Sheet (268 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.0683 mL | 5.3415 mL | 10.6831 mL | 26.7077 mL |
| 5 mM | 0.2137 mL | 1.0683 mL | 2.1366 mL | 5.3415 mL | |
| 10 mM | 0.1068 mL | 0.5342 mL | 1.0683 mL | 2.6708 mL | |
| 15 mM | 0.0712 mL | 0.3561 mL | 0.7122 mL | 1.7805 mL | |
| 20 mM | 0.0534 mL | 0.2671 mL | 0.5342 mL | 1.3354 mL | |
| 25 mM | 0.0427 mL | 0.2137 mL | 0.4273 mL | 1.0683 mL | |
| 30 mM | 0.0356 mL | 0.1781 mL | 0.3561 mL | 0.8903 mL | |
| 40 mM | 0.0267 mL | 0.1335 mL | 0.2671 mL | 0.6677 mL | |
| 50 mM | 0.0214 mL | 0.1068 mL | 0.2137 mL | 0.5342 mL |
DBCO-PEG4-TAMRA Related Classifications
HY-D1070 Related Classifications
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