DMG-PEG5000-Alkyne
DMG-PEG5000-Alkyne is a conjugate composed of dimyristic glycerol (DMG), a PEG chain, and a terminal alkyne group. DMG-PEG5000-Alkyne combines the membrane compatibility of phospholipids with the high reactivity of the alkyne group in click chemistry, making it suitable for research in biomaterial construction and drug delivery.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Molecular Weight:5000 (Average)
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
- Protokoll
- Reinheit & Dokumentation
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
The structure of DMG-PEG-Alkyne comprises three key components that work synergistically:
DMG: Acts as a hydrophobic anchor, securely embedding the entire molecule into the membrane structure of lipid nanoparticles.
PEG chain: Acts as a hydrophilic spacer, forming a hydration layer on the particle surface, reducing nonspecific adsorption and immune system clearance, thus prolonging the carrier's circulation time in vivo.
Alkyne group: Can undergo click chemistry reactions, achieving specific linkage with molecules containing an azide group.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 5000 (Average)
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SMILES
C#CCOCCOCC(OC(CCCCCCCCCCCCC)=O)COC(CCCCCCCCCCCCC)=O.[n]
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Reinheit & Dokumentation
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
DMG-PEG5000-Alkyne Related Classifications
HY-183191C Related Classifications
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