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Art. -Nr.: HY-125442

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Salinamide A

Salinamide A is a bacterial RNA polymerase inhibitor with IC₅₀: 0.2 μM against Staphylococcus aureus; 0.2 μM against Escherichia coli. Salinamide A inhibits transcription initiation and elongation, as well as bacterial RNA and protein synthesis without affecting DNA synthesis. Salinamide A can be used in studies related to the N-terminal hinge conformation and function of the RNAP bridge helix, and bacterial infections.

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CAS. Nr.: 152340-22-2
Formel: C₅₁H₆₉N₇O₁₅
Molecular Weight:1020.13

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Salinamide A Chemical Structure

CAS. Nr.: 152340-22-2

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
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Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
Vero E6	MIC	> 50 μg/mL	Growth-inhibitory activity against mammalian Vero E6 ATCC CRL1586 cells measured by broth microdilution assay with incubation for 16-48 hr at 37°C with 7% CO₂ atmosphere. Growth-inhibitory activity against mammalian Vero E6 ATCC CRL1586 cells measured by broth microdilution assay with incubation for 16-48 hr at 37°C with 7% CO₂ atmosphere.	24843001

In Vitro

Salinamide A (1.56-50 μg/mL; 16-48 hr) inhibits the growth of Gram-positive and Gram-negative pathogenic bacteria (it exhibits the strongest activity against Enterobacter cloacae ATCC 13047, with an MIC = 1.56 μg/mL)^[1].
Salinamide A (>50 μg/mL; 16-48 hr) exhibits no cytotoxicity against mammalian Vero E6 ATCC CRL1586 cells^[1].
Salinamide A (0-100 μM; 10 min) potently inhibits RNAP from Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, with an IC₅₀ of 0.2 μM for both, and does not inhibit RNAP from Thermus thermophilus or human RNAP I/II/III even at concentrations as high as 100 μM^[1].
Salinamide A (0.098 μg/mL; 10-30 min) inhibits RNA synthesis in Escherichia coli D21f2tolC cells within 10 min, suppresses protein synthesis at subsequent time points, and exerts no effect on DNA synthesis^[1].
Spontaneous resistance mutations induced in Escherichia coli D21f2tolC by salinamide A (0.6-1.2 μg/mL; 24-48 hr) localize exclusively to the RNA polymerase (RNAP) subunit genes rpoC and rpoB, and confer ≥ 16-fold resistance to the strain^[1].
Salinamide A (10 μM; 5 min) does not inhibit the formation of heparin-resistant transcription initiation open complexes between Escherichia coli RNAP holoenzyme and lacUV5 (ICAP) promoter DNA^[1].
Salinamide A (10 μM; 10 min) inhibits nucleotide addition during primer-dependent transcription initiation^[1].
Salinamide A (10 μM; 5 min) inhibits nucleotide addition during transcription elongation by Escherichia coli RNA polymerase holoenzyme and suppresses pyrophosphorolysis^[1].
Salinamide A (0-0.4 μM; 30 min) inhibits nucleotide addition by the Escherichia coli RNA polymerase holoenzyme in a non-competitive manner, with a K_i of 0.2 μM^[1].
Salinamide A (0-64 μM; 5 min) exerts equivalent inhibitory effects on the nucleotide addition process of wild-type and trigger loop-deleted (ΔTL) Escherichia coli RNAP core enzymes^[1].
Salinamide A (20 mM; 30 min) binds to the bridge helix cap structure of Escherichia coli RNA polymerase holoenzyme^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 152340-22-2
Molecular Weight 1020.13
Formel C₅₁H₆₉N₇O₁₅
SMILES

C[C@H]([C@@]1(CO1)/C=C\C(NCC(OC[C@@]2(NC([C@]([H])(NC([C@@H](N3C)CC4=CC=CC=C4)=O)[C@@H](C)O)=O)[H])=O)=O)OC(C=C5)=CC=C5[C@@](NC(C([C@H](CC)C)NC([C@H]([C@H](OC2=O)C)NC([C@@H]([C@@H](C(C)C)O)C)=O)=O)=O)([H])C3=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Degen D, et al. Transcription inhibition by the depsipeptide antibiotic salinamide A. eLife. 2014 Apr 30;3:e02451.

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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