2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. Calcium Channel
  4. Coelenterazine h hydrochloride

Coelenterazine h hydrochloride  (Synonyms: 2-Deoxycoelenterazine hydrochloride; CLZN-h hydrochloride)

Cat. No.: HY-D1024A Purity: 96.47%
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Coelenterazine h (2-Deoxycoelenterazine) hydrochloride, a coelenterazine derivative, is a luminescent substrate for RLuc8. Coelenterazine h hydrochloride is more sensitive to Ca2+, thus providing a valuable tool for measuring small changes in Ca2+ concentrations (Ex/Em = 437/466 nm).

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Based on 3 publication(s) in Google Scholar

Other Forms of Coelenterazine h hydrochloride:

Coelenterazine h hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Coelenterazine h hydrochloride purchased from MedChemExpress. Usage Cited in: ACS Pharmacol Transl Sci. 2025 Apr 8;8(7):1996-2008.  [Abstract]

    Coelenterazine h (5 μM; 5 min). Concentration-dependent inhibition of forskolin-induced (5 μM) cAMP accumulation using a BRET-Epac biosensor.

    Coelenterazine h hydrochloride purchased from MedChemExpress. Usage Cited in: Stress Biol. 2024 Feb 16;4(1):14.  [Abstract]

    A. tumefaciens strains carrying NlucN-FLAG, along with avrRpt2-HA-HiBiT or avrPto-HA-HiBiT, were infiltrated into the leaves. Thirty hours after infiltration, leaves discs were collected and incubated with Coelenterazine h (10 µM; 10 min) in a 96-well plate, then the luminescence was monitored using EnSpire Multimode Plate Reader.

    Coelenterazine h hydrochloride purchased from MedChemExpress. Usage Cited in: Stress Biol. 2024 Feb 16;4(1):14.  [Abstract]

    Leaves of transgenic A. thaliana plants expressing NlucN were infiltrated with H2O or 1 μM flg22. 24 hours after first infiltration, indicated bacteria (OD600 = 0.1) was infiltrated to the same leaves. Leaf discs were harvested 9 h later, and incubated with Coelenterazine h (10 µM; 10 min) in a 96-well plate, then the luminescence was monitored using EnSpire Multimode Plate Reader.

    Coelenterazine h hydrochloride purchased from MedChemExpress. Usage Cited in: Stress Biol. 2024 Feb 16;4(1):14.  [Abstract]

    Effector secretion was induced in vitro. Culture supernatants were collected, incubated with Coelenterazine h (10 μM; 10 min) and NlucN-His, and measured for luminescence. Total protein was analyzed by immunoblot using anti-AvrPto and anti-HA antibodies to detect the accumulation of AvrPto-HiBiT and AvrRpt2-HA-HiBiT. Signal-to-noise ratio was shown.

    Coelenterazine h hydrochloride purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2024 Jan;300(1):105527.  [Abstract]

    Coelenterazine h (10 μM; 2 h). HEK293T cells transfected with the empty vector (no opsin) served as the negative control, while those transfected with H. sapiens Opn4 were used as the positive control to measure the luminescence of Gαq-mediated Ca2+ increase.

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    Description

    Coelenterazine h (2-Deoxycoelenterazine) hydrochloride, a coelenterazine derivative, is a luminescent substrate for RLuc8. Coelenterazine h hydrochloride is more sensitive to Ca2+, thus providing a valuable tool for measuring small changes in Ca2+ concentrations (Ex/Em = 437/466 nm)[1][2][3][4].

    In Vitro

    Coelenterazine h (1-10 μM) hydrochloride can be used as the luminescent substrate for RLuc8[4].
    In the measurements of Ca2+ binding kinetics of BRAC, emission intensity of Venus (530 nm) from BRAC are monitored at 1 kHz just after rapid mixing of 5 nM BRAC protein with 20 μM coelenterazine-h hydrochloride in various concentration of Ca2+ buffer[4].
    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    1. Solution preparation[4]
    1.1 Preparation of stock solution
    Solvent: methanol or ethanol
    Concentration: 10 mM (optimized according to the experiment).
    1.2 Preparation of working solution
    Dilute to 10 μM with PBS or cell culture medium (optimized according to the experiment).
    Note: The stock solution and working solution should be prepared and used immediately. Keep it away from light.
    2. Cell staining
    2.1 Hela cells are transfected with BRAC or G5A.
    2.2 Add 10 μM Coelenterazine h hydrochloride into the cell plate.
    2.3 Incubate the dye-loading plate in a cell incubator for 1-4 h (optimized according to the experiment).
    2.4 Fluorescence microscopy detection (Ex/Em = 437/466 nm).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Coelenterazine h hydrochloride Related Antibodies
    Molecular Weight

    443.92

    Formula

    C26H22ClN3O2
    Appearance

    Solid

    Color

    Light yellow to orange

    SMILES

    OC(C=C1)=CC=C1C2=CN(C(C(CC3=CC=CC=C3)=N4)=O)C4=C(N2)CC5=CC=CC=C5.[H]Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    Ethanol : ≥ 50 mg/mL (112.63 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2527 mL 11.2633 mL 22.5266 mL
    5 mM 0.4505 mL 2.2527 mL 4.5053 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol 1 mM 2.2527 mL 11.2633 mL 22.5266 mL 56.3165 mL
    5 mM 0.4505 mL 2.2527 mL 4.5053 mL 11.2633 mL
    10 mM 0.2253 mL 1.1263 mL 2.2527 mL 5.6316 mL
    15 mM 0.1502 mL 0.7509 mL 1.5018 mL 3.7544 mL
    20 mM 0.1126 mL 0.5632 mL 1.1263 mL 2.8158 mL
    25 mM 0.0901 mL 0.4505 mL 0.9011 mL 2.2527 mL
    30 mM 0.0751 mL 0.3754 mL 0.7509 mL 1.8772 mL
    40 mM 0.0563 mL 0.2816 mL 0.5632 mL 1.4079 mL
    50 mM 0.0451 mL 0.2253 mL 0.4505 mL 1.1263 mL
    60 mM 0.0375 mL 0.1877 mL 0.3754 mL 0.9386 mL
    80 mM 0.0282 mL 0.1408 mL 0.2816 mL 0.7040 mL
    100 mM 0.0225 mL 0.1126 mL 0.2253 mL 0.5632 mL
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    Coelenterazine h hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Coelenterazine h2-DeoxycoelenterazineCLZN-hCalcium ChannelCa2+ channelsCa channelsLuminescent SubstratecalciumInhibitorinhibitorinhibit

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