Cyclo(Val-Val-Ile-d-Ala-Trp(Bodipy)-Gly)
Cyclo (Val-Val-Ile-d-Ala-Trp (Bodipy)-Gly) is a cyclic peptide-BODIPY conjugate. Cyclo (Val-Val-Ile-d-Ala-Trp (Bodipy)-Gly) detects prefibrillar Aβ1-42 aggregates in neuronal cells, generating perinuclear punctate fluorescent spots. Cyclo (Val-Val-Ile-d-Ala-Trp (Bodipy)-Gly) is applicable to Alzheimer's disease-related research.
For research use only. We do not sell to patients.
- Formula: C57H72BF2N9O6
- Molecular Weight:1028.05
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Cyclo (Val-Val-Ile-d-Ala-Trp (Bodipy)-Gly) efficiently detects early fibril-precursor Aβ1-42 oligomers in cell-free aggregation assays. Its fluorescence enhancement effect outperforms that of taBODIPY under both pH 7.4 and pH 8.0 aggregation conditions. In addition, it shows high selectivity for Aβ1-42 oligomers but low selectivity for IAPP oligomers[1].
Cyclo (Val-Val-Ile-d-Ala-Trp (Bodipy)-Gly) binds to monomeric Aβ1-42, and its apparent Kd value is determined to be 0.07 μM by fluorescence spectroscopy titration[1].
Cyclo (Val-Val-Ile-d-Ala-Trp (Bodipy)-Gly) (10 μM; 2 h) effectively detects intracellular Aβ1-42 aggregates in SH-SY5Y cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SH-SY5Y human neuroblastoma cells
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Concentration:10 μM
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Incubation Time:2 h
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Result:Produced distinct punctate fluorescent spots localized intracellularly, concentrated around the perinuclear region, indicating detection of aggregated Aβ1-42 species.
Produced clearer, more localized signals than conventional D54 and OC antibodies, which showed weak, diffuse cytoplasmic signals with no well-defined puncta.
Chemical Information
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Molecular Weight 1028.05
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Formula C57H72BF2N9O6
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Sequence
Cyclo(Val-Val-Ile-{d-Ala-Trp(Bodipy)}-Gly)
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Sequence Shortening
Cyclo(VVI-{d-Ala-Trp(Bodipy)}-G)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cyclo(Val-Val-Ile-d-Ala-Trp(Bodipy)-Gly) Related Classifications
HY-P11912 Related Classifications
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