Dehydrotrametenonic acid
Dehydrotrametenonic acid is a DNA topoisomerase II inhibitor with an IC50 of 37.5 μM. Dehydrotrametenonic acid inhibits DNA polymerase α and β with IC50 values of 45.5 and 86.5 μM, respectively. Dehydrotrametenonic acid does not intercalate into DNA and exhibits an inhibitory factor-like Topo II inhibition mechanism. Dehydrotrametenonic acid can be used in DNA replication, cell cycle, and cancer-related research.
For research use only. We do not sell to patients.
- CAS No.: 796062-77-6
- Formula: C30H44O3
- Molecular Weight:452.68
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
All Topoisomerase Isoforms
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Biological Activity
Description
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DNA polymerase α 45.5 μM (IC50) |
DNA Polymerase β 86.5 μM (IC50) |
Topoisomerase II 37.5 μM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| NUGC-3 | LD50 |
63.6 μM
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Cytotoxicity against human gastric cancer NUGC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
Cytotoxicity against human gastric cancer NUGC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
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14981314 |
In Vitro
Dehydrotrametenonic acid (compound 1) inhibits the activity of purified calf thymus DNA polymerase α, with an IC50 value of 45.5 μM[2].
Dehydrotrametenonic acid inhibits the activity of recombinant rat DNA polymerase β with an IC50 of 86.5 μM[2].
Dehydrotrametenonic acid (compound 5) inhibits the activity of human DNA topoisomerase II with an IC50 value of 37.5 μM, and does not inhibit the activity of human DNA topoisomerase I[4].
Dehydrotrametenonic acid (45.5-86.5 μM; 60 min) weakly inhibits the activity of all tested mammalian DNA polymerases (IC50 values 45.5-86.5 μM), and does not inhibit non-mammalian DNA metabolic enzymes[4].
Dehydrotrametenonic acid acts as a non-competitive inhibitor of calf thymus DNA polymerase α, and exhibits competitive inhibitory activity against rat DNA polymerase β relative to template-primer and nucleotide substrates[4].
Dehydrotrametenonic acid (100 μM) does not bind to or intercalate into calf thymus double-stranded DNA at a concentration of 100 μM[4].
Dehydrotrametenonic acid (48 h) inhibits the growth of NUGC-3 human gastric cancer cells, with an LD50 of 63.6 μM after 48 h of incubation[4].
Dehydrotrametenonic acid induces cell cycle arrest in the human gastric cancer cell line NUGC-3[4].
Dehydrotrametenonic acid induces apoptotic DNA fragmentation in human gastric cancer cell line NUGC-3[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:NUGC-3 human gastric cancer cells
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Concentration:100 μM (single-dose cytotoxicity); variable concentrations (dose-response analysis)
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Incubation Time:48 h
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Result:Strongly reduced NUGC-3 cell viability at 100 μM.
Showed dose-dependent inhibition of cell growth, with an LD50 of 63.6 μM.
Chemical Information
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CAS No. 796062-77-6
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Molecular Weight 452.68
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Formula C30H44O3
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SMILES
C[C@]12C=3C([C@]4(C)[C@@](CC3)(C(C)(C)C(=O)CC4)[H])=CC[C@]1(C)[C@@]([C@@H](CCC=C(C)C)C(O)=O)(CC2)[H]
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Structure Classification
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Initial Source
Poria cocos Wolf
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Akihisa T, et al. Triterpene acids from Poria cocos and their anti-tumor-promoting effects. Journal of natural products. 2007 Jun;70(6):948-53. [Content Brief]
[3]. Bai L, et al. Lingguizhugan decoction inhibits the cleavage of LYVE-1 by MMP-9 and promotes lymphangiogenesis to improve myocardial infarction. Phytomedicine : international journal of phytotherapy and phytopharmacology. 2025 Jul 25;143:156863. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Dehydrotrametenonic acid Related Classifications
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