(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH  (Synonyms: Surfagon)

(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH (Surfagon, Mwt 1167.34 Da) is an agonist of gonadotropin-releasing hormone (GnRH). (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH can be used as an internal standard for the LC-MS analysis of leuprorelide acetate. (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH has potential applications in biochemical analysis and fertility^[1][2][3].

(Des-Gly10, D-Ala6, Pro-NHEt9)-LHRH as internal standard for leuprorelin acetate^[1]
(1) Add 10 μL 200 ng/mL (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH solution to 100 μL plasma sample.
(2) Add ice-cold acetonitrile to solution in (1) in two batches (500 µL each time).
(3) Stir at 2500 rpm for 10 min.
(4) Centrifugation at 12500 rpm (4℃) for 10min.
(5) Transfer the supernatant into a centrifuge tube, dried under nitrogen gas at 35℃, and finally add 100 µL 0.1% formic acid in water/acetonitrile: 90/10 (v/v) solution.
(6) The Thermo Scientific Vanquish UPLC system is connected to the Thermo Scientific Orbitrap Explorer 480 mass spectrometer. Using the Waters Acquity UPLC HSS T3 column (2.1 × 100 mm, 1.8 µm) separation at a temperature of 40℃.
(7) The mobile phase A is 0.1% formic acid in water, and the mobile phase B is 0.1% formic acid in acetonitrile solution. The gradient starts at 95% mobile phase A, decreases to 10% over 5 min, and then remains constant for 0.5 min. At 6 min, the column returned to 95% mobile phase A and is rebalanced for 2 min. The flow rate was 0.3 mL/min and the injection volume is 20 µL.
(8) The sample is ionized by 3500V electric spray. In positive-ion mode, m/z 605.3275 is leuprolide acetate, and m/z 584.3052 is the internal standard. Data are analyzed by Xcalibur software (version 4.4).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH (2 µg/kg; i.p.; single dose) significantly increases anxiety behavior in adult male rats with and without gonadectomy^[2].
(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH (50 µg; single dose) improves the fertility of cows after fertilization and prevents embryo death when combined with Ainil (30 µL/kg)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1167.32

C₅₆H₇₈N₁₆O₁₂

52435-06-0

O=C([C@H]1NC(CC1)=O)N[C@@H](CC2=CN=CN2)C(N[C@H](C(N[C@@H](CO)C(N[C@H](C(N[C@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N3[C@@H](CCC3)C(NCC)=O)=O)=O)=O)=O)CC4=CC=C(C=C4)O)=O)=O)CC5=CNC6=CC=CC=C56)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.