(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH  (Synonyms: Surfagon)

(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH (Surfagon, Mwt 1167.34 Da) is an agonist of gonadotropin-releasing hormone (GnRH). (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH can be used as an internal standard for the LC-MS analysis of leuprorelide acetate. (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH has potential applications in biochemical analysis and fertility^[1][2][3].

(Des-Gly10, D-Ala6, Pro-NHEt9)-LHRH as internal standard for leuprorelin acetate^[1]
(1) Add 10 μL 200 ng/mL (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH solution to 100 μL plasma sample.
(2) Add ice-cold acetonitrile to solution in (1) in two batches (500 μL each time).
(3) Stir at 2500 rpm for 10 min.
(4) Centrifugation at 12500 rpm (4°C) for 10min.
(5) Transfer the supernatant into a centrifuge tube, dried under nitrogen gas at 35°C, and finally add 100 μL 0.1% formic acid in water/acetonitrile: 90/10 (v/v) solution.
(6) The Thermo Scientific Vanquish UPLC system is connected to the Thermo Scientific Orbitrap Explorer 480 mass spectrometer. Using the Waters Acquity UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm) separation at a temperature of 40°C.
(7) The mobile phase A is 0.1% formic acid in water, and the mobile phase B is 0.1% formic acid in acetonitrile solution. The gradient starts at 95% mobile phase A, decreases to 10% over 5 min, and then remains constant for 0.5 min. At 6 min, the column returned to 95% mobile phase A and is rebalanced for 2 min. The flow rate was 0.3 mL/min and the injection volume is 20 μL.
(8) The sample is ionized by 3500V electric spray. In positive-ion mode, m/z 605.3275 is leuprolide acetate, and m/z 584.3052 is the internal standard. Data are analyzed by Xcalibur software (version 4.4).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH (2 μg/kg; i.p.; single dose) significantly increases anxiety behavior in adult male rats with and without gonadectomy^[2].
(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH (50 μg; single dose) improves the fertility of cows after fertilization and prevents embryo death when combined with Ainil (30 μL/kg)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1167.32

C₅₆H₇₈N₁₆O₁₂

52435-06-0

Solid

White to off-white

{Pyr}-His-Trp-Ser-Tyr-{d-Ala}-Leu-Arg-Pro-NHEt

{Pyr}-HWSY-{d-Ala}-LRP-NHEt

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Wan B, et al. Polymer source affects in vitro-in vivo correlation of leuprolide acetate PLGA microspheres. Int J Pharm. 2022 Sep 25;625:122032.  [Content Brief]

  [2]. Masalova OO, et al. Effect of Surfagon on Open Field and Elevated Plus Maze Behavior of Gonadectomized and Non-Gonadectomized Male Rats. Bull Exp Biol Med. 2019 Nov;168(1):52-54.  [Content Brief]

  [3]. Kraevskiy A Y, et al. Surfagon and Ketaprofen for increasing fertility and preventing embryonic death in cows after insemination AY[J]. Ukrainian Journal of Ecology, 2020, 10(4): 159-164.