Cell Staining Analysis

Dye 937 is an asymmetric anthocyanin dye that can be used to detect DNA in electrophoretic gels.

Rhodamine B carbohydrazide is a fluorescent derivative based on Rhodamine B (HY-Y0016) that contains the spirocyclic structure of Rhodamine B; it can be used for the detection of copper ions (Cu²⁺), mercury ions, peroxynitrite, hydroxyl radicals, and nitric oxide (NO).

510 Calibration probe is a chemiluminescent group that can be used as a probe for in vivo imaging studies, with an emission maximum around 520 nm.

Fluo-2 potassium is a cell-impermeant fluorescent calcium indicator. Fluo-2 potassium binds calcium (K_d: 0.37 μM) with excitation/emission wavelengths of 493/518 nm, respectively.

Rhod-2 sodium is a cell impermeant, red fluorescent calcium indicator. Rhod-2 sodium exhibits a significant shift in fluorescence intensity upon calcium binding (ex max=549 nm; calcium-free v. ex/em max=552/581 nm; calcium-bound). Unlike the UV-excitable indicators Fura-2 and Indo-1 (HY-D0121), there is no accompanying spectral shift.

Fura-2 pentasodium is a cell-impermeable scaled fluorescent dye that can be used for intracellular calcium imaging. Fura-2 pentasodium has an emission wavelength of 510 nm and excitation wavelengths of 340 nm or 380 nm and the ratio of 340/380 fluorescence intensity is proportional to the intracellular Ca²⁺ level.

5-JOE azide is a fluorescent dye with the emission in yellow region of the spectrum. The azide group can react with alkyne, BCN, DBCO via Click Chemistry to yield a stable triazole linkage.

DMT-BH1 amidite (BHQ-1 DMT amidite) is a true dark quencher with broad absorption curve which covers the visible spectrum with maximum in green to yellow region. It is used for the synthesis of dual labeled oligonucleotide probes for qPCR bearing 5'-quencher DusQ 1 and other FRET applications for multiplexing assays.

SIMA phosphoramidite, 6-isomer is a xanthene dye with spectral characteristics similar to those of HEX but with a higher quantum yield. SIMA has higher stability during deprotection under alkaline conditions.

BP Fluor 532 NHS ester is an amine reactive, yellow-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond. BP Fluor 532 is a bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of BP Fluor 532 is ideally suited for the frequency-doubled Nd:YAG laser line. BP Fluor 532 can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.

DMT-BH2 amidite (BHQ-2 DMT amidite) is a black quencher dye for the synthesis of dual-labeled oligonucleotide probes for qPCR bearing 5'-quencher. This quencher is ideal for HEX, JOE, ROX, Cyanine5, and other dyes with emissions in the orange and red parts of the spectrum.

Pyrene azide 2 is a compound derived from pyreneacetic acid, connecting a fluorescent pyrene molecule by a PEG2 linker. The PEG units improve the water solubility of the compound.

Complex 3 is a fluorescent dithiocarbazate-copper complex with anticancer activity, which localizes to mitochondria. Complex 3 displays excitation/emission maxima of 455-495/535 nm, respectively. Complex 3 inhibits the growth of BxPC-3, AsPC-1, PANC-1, and WI38 pancreatic cancer cells with IC₅₀ values of 0.74, 0.41, 0.62, and 2.06 µM, respectively. Complex 3 induces lipid peroxidation and mitochondrial rupture and shrinkage in AsPC-1 cells. Complex 3 also induces mitochondrial apoptosis and cytokine-cytokine receptor interaction dysfunction in AsPC-1 cells. Complex 3 reduces tumor volume in an AsPC-1 mouse xenograft model.

Fluorescein hydrazide (3,6-Dihydroxyxanthane) is a fluorescein dye that can be used for the fluorophotometric determination of hydrogen peroxide (H2O2) and other reactive oxygen species (ROS).

2-Amino-5-methoxybenzamidoxime is a chemical probe for detection of ketone, including aromatic ketones, hydroxyl ketones, cyclic ketones and aliphatic ketones. 2-Amino-5-methoxybenzamidoxime exhibits an excitation maximal wavelength of 389 nm and a emission maximal wavelength of 515 nm.

PSP is a two-photon fluorescent probe for selective and sensitive detection of H₂S_n in live cells and tumor spheroids (Ex/Em = 440/640 nm). PSP can be utilized in ferroptosis research.

Asante potassium green-1 (APG-1) TMA is a cell-impermeable K⁺ (potassium) sensitive fluorescent indicator (excitation/emission = 525/545 nm).

Asante potassium green-1 AM (APG-1 AM) is a cell-permeable K⁺-sensitive fluorescent indicator and potassium ion indicator that exhibits minimal interference from Na⁺ and pH at physiologically relevant intracellular levels. Asante potassium green-1 AM enables non-invasive monitoring and quantitative analysis of intracellular potassium ion concentrations (Ex/Em = 522/547 nm).

NBD-X PE is a derivative of NBD-PE, where the NBD part is replaced by NBD-X acid.

Disperse Red 343 is an azo dye widely used in the textile industry for dyeing polyester fabrics.