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United States

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Cat. No.: HY-176031

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Fluo-2 potassium

Fluo-2 potassium is a cell-impermeant fluorescent calcium indicator. Fluo-2 potassium binds calcium (K_d: 0.37 μM) with excitation/emission wavelengths of 493/518 nm, respectively.

For research use only. We do not sell to patients.

Formula: C₃₆H₂₇K₅N₂O₁₃
Molecular Weight:891.10

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (272 KB)

Fluo-2 potassium Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity

Description

Fluo-2 potassium is a cell-impermeant fluorescent calcium indicator. Fluo-2 potassium binds calcium (K_d: 0.37 μM) with excitation/emission wavelengths of 493/518 nm, respectively^[1].

Chemical Information

Molecular Weight 891.10
Formula C₃₆H₂₇K₅N₂O₁₃
SMILES

O=C1C=CC2=C(C3=C(OC2=C1)C=C(O[K])C=C3)C4=CC(OCCOC5=CC(C)=CC=C5N(CC(O[K])=O)CC(O[K])=O)=C(C=C4)N(CC(O[K])=O)CC(O[K])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Minta A, et al. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J Biol Chem. 1989 May 15;264(14):8171-8. PMID: 2498308. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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