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Cat. No.: HY-D3064

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510 Calibration probe

510 Calibration probe is a chemiluminescent group that can be used as a probe for in vivo imaging studies, with an emission maximum around 520 nm.

For research use only. We do not sell to patients.

CAS No.: 2365253-76-3
Formula: C₂₁H₂₃ClO₆
Molecular Weight:406.86

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (271 KB)

510 Calibration probe Chemical Structure

CAS No.: 2365253-76-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity

Description

510 Calibration probe is a chemiluminescent group that can be used as a probe for in vivo imaging studies, with an emission maximum around 520 nm^[1].

Chemical Information

CAS No. 2365253-76-3
Molecular Weight 406.86
Formula C₂₁H₂₃ClO₆
SMILES

COC1(C2=C(C(O)=C(C=C2)/C=C/C(O)=O)Cl)C3(OO1)C4CC5CC3CC(C5)C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Animals and Diseases

Bioluminescent/Fluorescent Imaging Xenograft
Bioluminescent and fluorescent imaging xenograft models use tumor cells engineered to express optical reporters so tumor engraftment, growth, dissemination, and treatment response can be monitored longitudinally in living animals and validated ex vivo. Bioluminescence imaging usually measures luciferase activity after substrate administration and is commonly used as a surrogate for viable reporter-expressing tumor burden, while fluorescence imaging measures reporter or probe emission and can support tumor localization, ex vivo confirmation, or complementary multimodal analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Liao K, et al. Dihydroxanthene-Derived Dioxetane Chemiluminophores for Activatable in Vivo Imaging. Angew Chem Int Ed Engl. 2025 Oct 20;64(43):e202514236. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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