Thionin Staining Solution (1%)

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Cell Sample Staining
1.1 Collect cell samples, centrifuge at 1500 g for 10–15 min, and discard the supernatant.
1.2 Add fresh fixative (methanol:glacial acetic acid = 3:1), mix gently, and incubate at room temperature for 30 min.
1.3 Centrifuge again at 1500 g for 10–15 min and discard the supernatant.
1.4 Depending on the cell quantity, add a few drops of freshly prepared fixative and mix thoroughly to form a suspension.
1.5 Place 1 drop of the suspension onto a clean, pre-cooled microscope slide and allow it to air-dry.
1.6 Staining results: Metachromatic substances appear purplish-red; others appear blue.

2. Chromatin Staining
2.1 Place the slide specimen in 1 M HCl and incubate at 37°C for 20 min.
2.2 Rinse thoroughly with distilled water and allow to air-dry.
2.3 Immerse the slide specimen in thionine staining solution (0.4%) for 15–20 min.
2.4 Rinse with distilled water and allow to air-dry.
2.5 Observe under a microscope.
2.6 Staining results: Metachromatic substances appear purplish-red; others appear blue.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.