610CP TFA
Based on 1 Customer Validation
610CP TFA is a new type of actin labeling dye. It dissolves in organic solvents. In DMSO the 610CP TFA excitation/emission wavelength is between 609 and 634 nm. 610CP TFA is a fluorescent dye that penetrates living cells. Upon cell entry, 610CP TFA binds to Bromo-des-methyl-Jasplakinolide Therefore, 610CP TFA dye can be used to stain actin fluorescence images with low background and high resolution.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 95.56%
- Fòrmula: C30H29F3N2O6
- Peso molecular:570.56
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
In Vitro
Guide (The following is our recommended protocol. This protocol only provides guidance and should be modified according to your specific needs).
1. Preparation of 610CP TFA working solution
1.1 Preparation of stock solution
Prepare a 10 mM 610CP TFA stock solution using DMSO.
Note: It is recommended to aliquot the 610CP TFA stock solution and store at -20°C or -80°C, protected from light.
1.2 Preparation of working solution
Dilute the stock solution with pre-warmed serum-free cell culture medium or PBS to prepare a 5-10 μM 610CP TFA working solution.
Note: Please adjust the concentration of the 610CP TFA working solution according to actual conditions and prepare fresh before use.
2. Cell staining (suspension cells)
2.1 Collect cells by centrifugation, wash twice with PBS for 5 minutes each time. Adjust the cell density to 1×106/mL.
2.2 Add 1 mL 610CP TFA working solution and incubate at room temperature for 30-60 minutes.
2.3 Centrifuge at 400 g for 3-4 minutes and discard the supernatant.
2.4 Wash the cells twice with PBS for 5 minutes each time.
2.5 Resuspend the cells in 1 mL serum-free medium or PBS, then observe using a fluorescence microscope or flow cytometer.
3. Cell staining (adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslips from the culture medium and aspirate excess medium.
3.3 Add 100 μL staining working solution, gently shake to ensure complete coverage of the cells, and incubate for 30-60 minutes.
3.4 Remove the staining working solution and wash with culture medium 2-3 times for 5 minutes each time, then observe using a fluorescence microscope.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Powder
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Peso molecular 570.56
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Fòrmula C30H29F3N2O6
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SMILES
O=C([O-])C1=C(C=C(C=C1)C(O)=O)C(C2=C(C=C(C=C2)N(C)C)C(C)3C)=C(C=C/4)C3=CC4=[N+](C)/C.O=C(C(F)(F)F)O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 25 mg/mL (43.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
Referencias
[1]. Vladimir N Belov, et al. Synthesis of Fluorescent Jasplakinolide Analogues for Live-Cell STED Microscopy of Actin. J Org Chem. 2020 Jun 5;85(11):7267-7275. [Content Brief]
[2]. Vladimir N Belov, et al. Synthesis of Fluorescent Jasplakinolide Analogues for Live-Cell STED Microscopy of Actin. J Org Chem. 2020 Jun 5;85(11):7267-7275. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7527 mL | 8.7633 mL | 17.5266 mL | 43.8166 mL |
| 5 mM | 0.3505 mL | 1.7527 mL | 3.5053 mL | 8.7633 mL | |
| 10 mM | 0.1753 mL | 0.8763 mL | 1.7527 mL | 4.3817 mL | |
| 15 mM | 0.1168 mL | 0.5842 mL | 1.1684 mL | 2.9211 mL | |
| 20 mM | 0.0876 mL | 0.4382 mL | 0.8763 mL | 2.1908 mL | |
| 25 mM | 0.0701 mL | 0.3505 mL | 0.7011 mL | 1.7527 mL | |
| 30 mM | 0.0584 mL | 0.2921 mL | 0.5842 mL | 1.4606 mL | |
| 40 mM | 0.0438 mL | 0.2191 mL | 0.4382 mL | 1.0954 mL |
610CP TFA Related Classifications
HY-D1346A Related Classifications
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