Cat. No.: HY-B0617

S-Adenosyl-L-methionine

(Synonyms: S-Adenosyl methionine; Ademetionine; AdoMet)

22 Cited Publications

Customer Review

Based on 22 publication(s) in Google Scholar

S-Adenosyl-L-methionine (S-Adenosyl methionine) is an orally active methyl group donor. S-Adenosyl-L-methionine is a dietary supplement with potent antidepressant effects. S-Adenosyl-L-methionine also has anti‑proliferative, pro‑apoptotic and anti‑metastatic roles in cancers. S-Adenosyl-L-methionine has the potential for, cancer, liver disease and osteoarthritis research.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 29908-03-0
Formula: C₁₅H₂₂N₆O₅S
Molecular Weight:398.44

Storage:

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

S-Adenosyl-L-methionine Chemical Structure

CAS No.: 29908-03-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (22)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

S-Adenosyl-L-methionine (300 µM, 24 or 48 h) induces cell apoptosis, and promotes the cell cycle arrest in Cal-33 and JHU-SCC-011 cells^[4].
S-Adenosyl-L-methionine (300 µM, 24 h) decreases the migration of the Cal-33 and JHU-SCC-011 cells^[4].
S-Adenosyl-L-methionine (5-40 μg/mL, 48 h) protects the anticancer effect of 5‑FU by regulating the expression of DNMTs^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[4]

Cell Line:

Cal-33 and JHU-SCC-011 cells
Concentration:

300 µM
Incubation Time:

24 h (Cal-33) or 48 h (HU-SCC-011)
Result:

Showed an approximately 10% and 3%of apoptotic cells respectively.

Cell Cycle Analysis^[4]

Cell Line:

Cal-33 and JHU-SCC-011 cells
Concentration:

300 µM
Incubation Time:

24 h (Cal-33) or 48 h (HU-SCC-011)
Result:

Decreased the expression of cyclin B1, E1 and D1 in the Cal-33 and JHU-SCC-011 cells.

In Vivo

S-Adenosyl-L-methionine (30 mg/kg, p.o., for 3 days) prevents ASD like behaviors induced by early postnatal valproic acid exposure in young mice^[6].
S-Adenosyl-L-methionine (50 and 100 mg/kg, p.o.) shows antiepileptic, memory-enhancing, and antioxidant properties in a Pentylenetetrazole-induced rat epilepsy model^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Valproic acid treated young mice^[6]
Dosage:

30 mg/kg
Administration:

p.o., for 3 days
Result:

Alleviated most ASD like neurobehavioral symptoms.
Normalized the redox potential in the prefrontal cortex.

Chemical Information

CAS No. 29908-03-0
Appearance Solid
Molecular Weight 398.44
Formula C₁₅H₂₂N₆O₅S
Color White to light yellow
SMILES

O[C@H]1[C@](O[C@@H]([C@H]1O)C[S+](C)CC[C@H](N)C([O-])=O)([H])N2C3=NC=NC(N)=C3N=C2
Synonyms

S-Adenosyl methionine; Ademetionine; AdoMet
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Escherichia coli
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Publications (22)

All Cell Proliferation/Viability AssayFlow CytometryWB

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Journal Impact Factor

Mol Cancer

ZNF526 drives tumor growth by enhancing SHMT1-dependent serine metabolism and antioxidant capability in TNBC. [Abstract]

2025 Nov 20;24(1):295. PMID: 41267037
Autophagy

SETDB1 enhances starvation-induced lipophagy by inhibiting m6A-mediated mRNA decay via DDX5 methylation. [Abstract]

2026 May 17:1-23. PMID: 42087801
Nat Commun

Nuclear localization of MTHFD2 is required for correct mitosis progression. [Abstract]

2024 Nov 12;15(1):9529. PMID: 39532843
Gut Microbes

Gut microbiota-mediated betaine regulates skeletal muscle fiber type transition by affecting m6A RNA methylation and Myh7 expression. [Abstract]

2025 Dec;17(1):2545434. PMID: 40824213
Environ Sci Technol

Arsenic Exposure Triggers Nonalcoholic Fatty Liver Disease through Repressing S-Adenosylmethionine-Dependent Histone Methylation in Rats. [Abstract]

2025 Jan 14;59(1):268-278. PMID: 39746780
Pharmacol Res

Developing patient-derived organoids to demonstrate JX24120 inhibits SAMe synthesis in endometrial cancer by targeting MAT2B. [Abstract]

2024 Sep 16:107420. PMID: 39293586

S-Adenosyl-L-methionine purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Sep 16:107420. [Abstract]

Cell viability was determined in response to JX24120 with the supplement of exogenous S-Adenosyl-L-methionine disulfate tosylate (SAMe, 5-10 μM). The results demonstrated that SAMe effectively reversed the cytotoxicity induced by JX24120 in endometrial cancer organoids and cell lines.

S-Adenosyl-L-methionine purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Sep 16:107420. [Abstract]

The protein synthesis rates of treated organoids, ISK and KLE were examined by puromycin incorporation assay. In organoids and cell lines, JX24120 inhibited protein synthesis in a dose-dependent manner, which was also rescued by exogenous supplementation with S-Adenosyl-L-methionine disulfate tosylate (SAMe, 10 μM).
J Exp Med

Adenosine metabolic clearance maintains liver homeostasis by licensing arginine methylation of RIPK1. [Abstract]

2026 Jan 5;223(1):e20250603. PMID: 41081715
Int J Biol Macromol

Targeting OXCT1 with the methyl donor S-adenosylmethionine as a therapeutic strategy for cerebral cavernous malformations. [Abstract]

2026 Apr:355:151129. PMID: 41865434
J Agric Food Chem

Sesamol Epigenetically Induces Estrogen Receptor α Re-expression by Upregulating miR-370-3p in Estrogen Receptor α-Negative Breast Cancer. [Abstract]

2021 Aug 11;69(31):8737-8746. PMID: 34325508
Biochem Pharmacol

S-adenosylmethionine attenuates angiotensin II-induced aortic dissection formation by inhibiting vascular smooth muscle cell phenotypic switch and autophagy. [Abstract]

2024 Jan:219:115967. PMID: 38065291
Ind Crops Prod

2025 Dec 2;238:122414.
Food Funct

Methionine restriction attenuates renal injury by suppressing macrophage polarization via IRF1 downregulation. [Abstract]

2025 Oct 27. PMID: 41140247

S-Adenosyl-L-methionine purchased from MedChemExpress. Usage Cited in: Food Funct. 2025 Oct 27. [Abstract]

Representative flow cytometry plots of CD86⁺ and CD206⁺ BMDMs cultured under different treatment conditions (n = 5). The results showed that S-Adenosyl-L-methionine disulfate tosylate (SAM, 100 μM) supplementation reversed the aberrant polarization phenotype of BMDMs.
Plant J

Transcriptomic and metabolomic analyses unveil TaASMT3-mediated wheat resistance against stripe rust by promoting melatonin biosynthesis. [Abstract]

2025 Apr;122(2):e70182. PMID: 40298361
Int Immunopharmacol

Hydrogen sulfide ameliorates high glucose-induced pro-inflammation factors in HT-22 cells: Involvement of SIRT1-mTOR/NF-κB signaling pathway. [Abstract]

2021 Jun:95:107545. PMID: 33765609

S-Adenosyl-L-methionine purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2021 Jun:95:107545. [Abstract]

S-adenosylmethionine (SAMe) application at 30 min prior to high glucose treatment significantly increased H2S levels compared with the HG group. The cystathionine-β-synthase (CBS) level is obviously elevated in HG + SAMe group compared with the HG group.
Epigenetics Chromatin

Ethionine-mediated reduction of S-adenosylmethionine is responsible for the neural tube defects in the developing mouse embryo-mediated m6A modification and is involved in neural tube defects via modulating Wnt/β-catenin signaling pathway. [Abstract]

2021 Dec 4;14(1):52. PMID: 34863249
J Pharm Biomed Anal

Quantitative determination of ademetionine in tablets utilizing ATR-FTIR and partial least squares methods approaches. [Abstract]

2024 Apr 15:241:115991. PMID: 38301577
SSRN

2026 Jun 23.
bioRxiv

2025 Jun 27.
Biomed Pharmacother

Identification of nsp16 inhibitors of SARS -CoV-2, SARS -CoV-1 and MERS-CoV from FDA-approved drugs using in silico and in vitro methods. [Abstract]

2025 Jun 20:189:118246. PMID: 40543162
bioRxiv

2023 Jun 1.
Research Square Preprint

2021 Jun.
Research Square Preprint

2020 Aug.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (250.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 43 mg/mL (107.92 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5098 mL

12.5489 mL

25.0979 mL

5 mM

0.5020 mL

2.5098 mL

5.0196 mL

10 mM

0.2510 mL

1.2549 mL

2.5098 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.27 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. G M Bressa. S-adenosyl-l-methionine (SAMe) as antidepressant: meta-analysis of clinical studies. Acta Neurol Scand Suppl. 1994;154:7-14. [Content Brief]

[2]. Wadie I Najm, et al. S-adenosyl methionine (SAMe) versus celecoxib for the treatment of osteoarthritis symptoms: a double-blind cross-over trial. [ISRCTN36233495]. BMC Musculoskelet Disord. 2004 Feb 26;5:6. [Content Brief]

[3]. Shelly C Lu, et al. S-adenosylmethionine in liver health, injury, and cancer. Physiol Rev. 2012 Oct;92(4):1515-42. [Content Brief]

[4]. Mosca L, et al. Effects of S‑adenosyl‑L‑methionine on the invasion and migration of head and neck squamous cancer cells and analysis of the underlying mechanisms. Int J Oncol. 2020 May;56(5):1212-1224. [Content Brief]

[5]. Ham MS, et al. S-adenosyl methionine specifically protects the anticancer effect of 5-FU via DNMTs expression in human A549 lung cancer cells. Mol Clin Oncol. 2013 Mar;1(2):373-378. [Content Brief]

[6]. Ornoy A, et al. S-adenosyl methionine prevents ASD like behaviors triggered by early postnatal valproic acid exposure in very young mice. Neurotoxicol Teratol. 2019 Jan-Feb;71:64-74. [Content Brief]

[7]. Dhediya RM, et al. Evaluation of antiepileptic effect of S-adenosyl methionine and its role in memory impairment in pentylenetetrazole-induced kindling model in rats. Epilepsy Behav. 2016 Aug;61:153-157. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.5098 mL	12.5489 mL	25.0979 mL	62.7447 mL
	5 mM	0.5020 mL	2.5098 mL	5.0196 mL	12.5489 mL
	10 mM	0.2510 mL	1.2549 mL	2.5098 mL	6.2745 mL
	15 mM	0.1673 mL	0.8366 mL	1.6732 mL	4.1830 mL
	20 mM	0.1255 mL	0.6274 mL	1.2549 mL	3.1372 mL
	25 mM	0.1004 mL	0.5020 mL	1.0039 mL	2.5098 mL
	30 mM	0.0837 mL	0.4183 mL	0.8366 mL	2.0915 mL
	40 mM	0.0627 mL	0.3137 mL	0.6274 mL	1.5686 mL
	50 mM	0.0502 mL	0.2510 mL	0.5020 mL	1.2549 mL
	60 mM	0.0418 mL	0.2091 mL	0.4183 mL	1.0457 mL
	80 mM	0.0314 mL	0.1569 mL	0.3137 mL	0.7843 mL
	100 mM	0.0251 mL	0.1255 mL	0.2510 mL	0.6274 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

S-Adenosyl-L-methionine

22 Cited Publications

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S-Adenosyl-L-methionine

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Natural Product Library

FDA-Approved Drug Library

Human Endogenous Metabolite Compound Library

Drug Repurposing Compound Library

NMPA-Approved Drug Library

Orally Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Gut Microbial Metabolite Library

Microbial Metabolite Library

Food-Sourced Compound Library

Rare Diseases Drug Library

Antidepressant Compound Library

FDA-Approved Anticancer Drug Library

Human Metabolite Library

Heterocyclic Compound Library

Off-patent Drug Library

Animal-Sourced Natural Product Library

Highly Selective Activators Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Dietary Supplement Library

Non-natural Amino Acid Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Endogenous Metabolite Library

Mass Spectrometry Human Metabolite Library

Amino Acid Metabolite Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) S-Adenosyl-L-methionine

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (22)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

S-Adenosyl-L-methionine Related Classifications

HY-B0617 Related Classifications