Cat. No.: HY-N0666

L-Aspartic acid

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

L-Aspartic acid is a NMDA receptor agonist and acidic amino acid. L-Aspartic acid has no independent analgesic activity. L-Aspartic acid can antagonize the analgesic effects of D-Aspartic acid and Morphine. L-Aspartic acid regulates polymorph transformation, crystal growth/aggregation and nucleation processes of calcium carbonate, and can serve as a biomineralization template. L-Aspartic acid promotes burst firing of central neurons. L-Aspartic acid can be used in studies related to cerebral ischemia and epilepsy.

For research use only. We do not sell to patients.

Purity: 99.88%
CAS No.: 56-84-8
Formula: C₄H₇NO₄
Molecular Weight:133.10

Storage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

L-Aspartic acid Chemical Structure

CAS No.: 56-84-8

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

NMDA Receptor

All iGluR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	>1000 μM Compound: (S)-1	Agonistic activity at mGlu2 receptor expressed in CHO cells Agonistic activity at mGlu2 receptor expressed in CHO cells	[PMID: 8759641]
CHO	EC₅₀	>1000 μM Compound: (S)-1	Agonistic activity at mGlu6 receptor expressed in CHO cells Agonistic activity at mGlu6 receptor expressed in CHO cells	[PMID: 8759641]
CHO	EC₅₀	>1000 μM Compound: (S)-1	Agonistic activity at mGlu1-alpha receptor expressed in CHO cells Agonistic activity at mGlu1-alpha receptor expressed in CHO cells	[PMID: 8759641]
HEK293	EC₅₀	398 μM Compound: L-asp	Inhibition of the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by a phenotypic impedance-based assay detecting changes in cell morphology by L-glutamate uptake in HEK-293 JumpIN-SLC1A3 cells (PubChem AID: 1745862) Inhibition of the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by a phenotypic impedance-based assay detecting changes in cell morphology by L-glutamate uptake in HEK-293 JumpIN-SLC1A3 cells (PubChem AID: 1745862)	[PMID: 35677430]
HEK293	EC₅₀	15 μM Compound: 5960	Substrate uptake by the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A3 cells (PubChem AID: 1745864) Substrate uptake by the Excitatory Amino Acid Transporter 1 (EAAT1, SLC1A3) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC1A3 cells (PubChem AID: 1745864)	10.5281/zenodo.6782621

In Vitro

L-Aspartic acid (L-[³H]Asp; 0.05 μCi/mL; 1-120 min) is taken up by rat brain slices, with a slice-to-medium ratio of 2.53 mL/g brain tissue at 120 minutes and an initial uptake rate of 1.22 mL/min/g brain tissue^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

L-Aspartic acid (i.p., 55-230 mg/kg) exhibits no intrinsic analgesic activity at intraperitoneal doses up to 230 mg/kg, but significantly antagonizes the analgesic effects of D-Aspartic acid and Morphine at the intraperitoneal dose of 230 mg/kg^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/C (both sexes, 25-40 g)^[1]
Dosage:

55 mg/kg; 115 mg/kg; 230 mg/kg
Administration:

i.p.
Result:

Did not cause any antinociceptive effect at doses of 55, 115, and 230 mg/kg.
Significantly inhibited the antinociceptive effect of 115 mg/kg D-aspartic acid at 230 mg/kg.
Shortened the duration of the antinociceptive effect of 5 mg/kg morphine at 230 mg/kg.

Chemical Information

CAS No. 56-84-8
Appearance Solid
Molecular Weight 133.10
Formula C₄H₇NO₄
Color White to off-white
SMILES

N[C@H](C(O)=O)CC(O)=O
Structure Classification
- Amino acids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Publications (5)

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Nat Protoc

An unbiased method for evaluating the genome-wide specificity of base editors in rice. [Abstract]

2021 Jan;16(1):431-457. PMID: 33349703
Redox Biol

Enteric coronavirus PDCoV evokes a non-Warburg effect by hijacking pyruvic acid as a metabolic hub. [Abstract]

2024 May:71:103112. PMID: 38461791

L-Aspartic acid purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 May:71:103112. [Abstract]

IPI-2I cells were transfected with an empty vector or the eukaryotic expression plasmid pCAGGS-Flag-SLC1A3, and then infected with PDCoV (MOI = 0.5) in glutamine-containing medium, glutamine-free medium, or glutamine-free medium supplemented with L-Aspartic acid (Asp: 4 mM) or Glu (4 mM).
FASEB J

Maternal LDHB Safeguards Redox Balance and Developmental Competence During Preimplantation Embryo Cleavage. [Abstract]

2026 May 31;40(10):e71888. PMID: 42117986
Amino Acids

Identification of amino acids metabolomic profiling in human plasma distinguishes lupus nephritis from systemic lupus erythematosus. [Abstract]

2024 Sep 18;56(1):56. PMID: 39292313

Solvent & Solubility

In Vitro:

1M NaOH : 100 mg/mL (751.31 mM; ultrasonic and adjust pH to 12 with NaOH)

H₂O : 2 mg/mL (15.03 mM; ultrasonic and warming and heat to 60°C)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

7.5131 mL

37.5657 mL

75.1315 mL

5 mM

1.5026 mL

7.5131 mL

15.0263 mL

10 mM

0.7513 mL

3.7566 mL

7.5131 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 1 mg/mL (7.51 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Onat F, et al. Antinociceptive effect of D-aspartic acid in mice. Pharmacology, biochemistry, and behavior. 1995 Aug;51(4):715-9. [Content Brief]

[2]. Hosoya K, et al. Blood-brain barrier produces significant efflux of L-aspartic acid but not D-aspartic acid: in vivo evidence using the brain efflux index method. Journal of neurochemistry. 1999 Sep;73(3):1206-11. [Content Brief]

[3]. Tong H, et al. Control over the crystal phase, shape, size and aggregation of calcium carbonate via a L-aspartic acid inducing process. Biomaterials. 2004 Aug;25(17):3923-9. [Content Brief]

[4]. MacDonald JF, et al. L-Aspartic acid induces a region of negative slope conductance in the current-voltage relationship of cultured spinal cord neurons. Brain research. 1982 Apr 08;237(1):248-53. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / 1M NaOH	1 mM	7.5131 mL	37.5657 mL	75.1315 mL	187.8287 mL
	5 mM	1.5026 mL	7.5131 mL	15.0263 mL	37.5657 mL
	10 mM	0.7513 mL	3.7566 mL	7.5131 mL	18.7829 mL
	15 mM	0.5009 mL	2.5044 mL	5.0088 mL	12.5219 mL
1M NaOH	20 mM	0.3757 mL	1.8783 mL	3.7566 mL	9.3914 mL
	25 mM	0.3005 mL	1.5026 mL	3.0053 mL	7.5131 mL
	30 mM	0.2504 mL	1.2522 mL	2.5044 mL	6.2610 mL
	40 mM	0.1878 mL	0.9391 mL	1.8783 mL	4.6957 mL
	50 mM	0.1503 mL	0.7513 mL	1.5026 mL	3.7566 mL
	60 mM	0.1252 mL	0.6261 mL	1.2522 mL	3.1305 mL
	80 mM	0.0939 mL	0.4696 mL	0.9391 mL	2.3479 mL
	100 mM	0.0751 mL	0.3757 mL	0.7513 mL	1.8783 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

L-Aspartic acid

5 Cited Publications

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L-Aspartic acid

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Publications Citing Use of MedChemExpress (MCE) L-Aspartic acid

All iGluR Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

L-Aspartic acid Related Classifications

HY-N0666 Related Classifications