2. Metabolic Enzyme/Protease PI3K/Akt/mTOR Apoptosis
  3. Endogenous Metabolite mTOR Apoptosis
  4. L-Proline

L-Proline is one of the twenty amino acids used in living organisms as the building blocks of proteins.

For research use only. We do not sell to patients.

CAS No. : 147-85-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of L-Proline:

L-Proline Related Antibodies

Top Publications Citing Use of Products

    L-Proline purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Oct 16;14(10):682.  [Abstract]

    Proline (100 μM, 12 h) rescued the protein expression of stemness-related factors in PYCR1-silencing MDA-MB-231 cells.

    L-Proline purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Oct 16;14(10):682.  [Abstract]

    Proline (100 μM, 12 h) rescued sphere formation capacity in PYCR1-silencing MDA-MB-231 cells.

    L-Proline purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Oct 16;14(10):682.  [Abstract]

    Proline (100 μM, 12 h) rescued ALDH+ subpopulations in PYCR1-silencing MDA-MB-231 cells.

    L-Proline purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Oct 16;14(10):682.  [Abstract]

    Proline (0.8 mg/kg, i.p., 24 days) rescued the inhibitory effect of PYCR1 silencing on xenograft PY8119 tumor growth.

    L-Proline purchased from MedChemExpress. Usage Cited in: Autophagy. 2023 Feb;19(2):632-643.  [Abstract]

    L-Proline (300 μM, 24 h) rescued the decreased COL1A1 expression in ATG3- or PRKN-deficient CAFs when added to the low-glucose medium.

    L-Proline purchased from MedChemExpress. Usage Cited in: Autophagy. 2023 Feb;19(2):632-643.  [Abstract]

    L-Proline (300 μM, 24 h) rescued collagen secretion in ATG3- or PRKN-deficient CAFs when added to the low-glucose medium.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    L-Proline is one of the twenty amino acids used in living organisms as the building blocks of proteins.

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    In Vitro

    L-Proline (1 g/L, 48 h) inhibits AFB1- or AFM1-induced apoptosis through downregulation of PRODH expression and its downstream apoptotic factors (Bax, Bcl-2 and cleaved Caspase-3)[1].
    L-Proline (40-400 μM, 4 days) activates the mTOR signaling pathway, induces the differentiation of mouse embryonic stem cells (ES cells)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Proline Related Antibodies

    Apoptosis Analysis[1]

    Cell Line: HEK293
    Concentration: 1 g/L
    Incubation Time: 48 h
    Result: Inhibited AFB1- or AFM1-induced apoptosis.
    In Vivo

    L-Proline (10 g/kg, po for 28 days) exhibits protective effect against renal oxidative damage and dysfunction caused by AFB1 and AFM1[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: CD-1 mouse AFB1 and AFM1 induced kidney damage model[1]
    Dosage: 10 g/kg
    Administration: po for 28 days
    Result: Ameliorated AFB1 and AFM1 induced renal oxidative damage and dysfunction.
    Clinical Trial
    Molecular Weight

    115.13

    Formula

    C5H9NO2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O)[C@H]1NCCC1
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    RT, protect from light, stored under nitrogen

    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (434.29 mM; Need ultrasonic)

    DMSO : 2 mg/mL (17.37 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 8.6858 mL 43.4292 mL 86.8583 mL
    5 mM 1.7372 mL 8.6858 mL 17.3717 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (868.58 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: ≥98.0%

    SDS (393 KB)

    COA (249 KB) HNMR (118 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 8.6858 mL 43.4292 mL 86.8583 mL 217.1458 mL
    5 mM 1.7372 mL 8.6858 mL 17.3717 mL 43.4292 mL
    10 mM 0.8686 mL 4.3429 mL 8.6858 mL 21.7146 mL
    15 mM 0.5791 mL 2.8953 mL 5.7906 mL 14.4764 mL
    H2O 20 mM 0.4343 mL 2.1715 mL 4.3429 mL 10.8573 mL
    25 mM 0.3474 mL 1.7372 mL 3.4743 mL 8.6858 mL
    30 mM 0.2895 mL 1.4476 mL 2.8953 mL 7.2382 mL
    40 mM 0.2171 mL 1.0857 mL 2.1715 mL 5.4286 mL
    50 mM 0.1737 mL 0.8686 mL 1.7372 mL 4.3429 mL
    60 mM 0.1448 mL 0.7238 mL 1.4476 mL 3.6191 mL
    80 mM 0.1086 mL 0.5429 mL 1.0857 mL 2.7143 mL
    100 mM 0.0869 mL 0.4343 mL 0.8686 mL 2.1715 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    L-Proline Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Proline147-85-3Endogenous MetabolitemTORApoptosisMammalian target of RapamycinInhibitorinhibitorinhibit

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