L-Proline
Based on 10 publication(s) in Google Scholar
L-Proline is one of the twenty amino acids used in living organisms as the building blocks of proteins.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 147-85-3
- Formula: C5H9NO2
- Molecular Weight:115.13
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Storage:
RT, protect from light, stored under nitrogen.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) L-Proline
More- Autophagy. 2023 Feb;19(2):632-643. [Abstract]
- Adv Sci (Weinh). 2025 Nov 21:e08472. [Abstract]
- Cell Death Dis. 2023 Oct 16;14(10):682. [Abstract]
- Information Processing in Agriculture. 2026 Mar 3.
- Int J Pharm. 2024 Apr 25:655:124055. [Abstract]
- Molecules. 2025 Mar 9;30(6):1224. [Abstract]
- Amino Acids. 2024 Sep 18;56(1):56. [Abstract]
- Biochem Biophys Res Commun. 2026 Jul 23:823:153971. [Abstract]
- bioRxiv. 2025 July 15.
- Res Sq. 2025 Jul 21.
Customer Validation & Images
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WB
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Bio/Physico-chemical Assay
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WB
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2D/3D Cell Culture and Differentiation
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Bio/Physico-chemical Assay
All Endogenous Metabolite Isoforms
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Biological Activity
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Microbial Metabolite |
Human Endogenous Metabolite |
L-Proline (1 g/L, 48 h) inhibits AFB1- or AFM1-induced apoptosis through downregulation of PRODH expression and its downstream apoptotic factors (Bax, Bcl-2 and cleaved Caspase-3)[1].
L-Proline (40-400 μM, 4 days) activates the mTOR signaling pathway, induces the differentiation of mouse embryonic stem cells (ES cells)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK293
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Concentration:1 g/L
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Incubation Time:48 h
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Result:Inhibited AFB1- or AFM1-induced apoptosis.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CD-1 mouse AFB1 and AFM1 induced kidney damage model[1]
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Dosage:10 g/kg
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Administration:po for 28 days
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Result:Ameliorated AFB1 and AFM1 induced renal oxidative damage and dysfunction.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 147-85-3
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Appearance Solid
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Molecular Weight 115.13
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Formula C5H9NO2
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Color White to off-white
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SMILES
O=C(O)[C@H]1NCCC1
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, protect from light, stored under nitrogen
In solvent -80°C 1 year -20°C 6 months
Publications (10)
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Journal Impact Factor
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Most Recent
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Autophagy
Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis. [Abstract]2023 Feb;19(2):632-643. PMID: 35786294
L-Proline purchased from MedChemExpress. Usage Cited in: Autophagy. 2023 Feb;19(2):632-643. [Abstract]
L-Proline (300 μM, 24 h) rescued the decreased COL1A1 expression in ATG3- or PRKN-deficient CAFs when added to the low-glucose medium.
L-Proline purchased from MedChemExpress. Usage Cited in: Autophagy. 2023 Feb;19(2):632-643. [Abstract]
L-Proline (300 μM, 24 h) rescued collagen secretion in ATG3- or PRKN-deficient CAFs when added to the low-glucose medium.
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Adv Sci (Weinh)
Targeted Inhibition of CD74+ Macrophages by Luteolin via CEBPB/P65 Signaling Ameliorates Osteoarthritis Progression. [Abstract]2025 Nov 21:e08472. PMID: 41268703 -
Cell Death Dis
Pyrroline-5-carboxylate reductase 1 reprograms proline metabolism to drive breast cancer stemness under psychological stress. [Abstract]2023 Oct 16;14(10):682. PMID: 37845207
L-Proline purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Oct 16;14(10):682. [Abstract]
Proline (100 μM, 12 h) rescued the protein expression of stemness-related factors in PYCR1-silencing MDA-MB-231 cells.
L-Proline purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Oct 16;14(10):682. [Abstract]
Proline (100 μM, 12 h) rescued sphere formation capacity in PYCR1-silencing MDA-MB-231 cells.
L-Proline purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Oct 16;14(10):682. [Abstract]
Proline (100 μM, 12 h) rescued ALDH+ subpopulations in PYCR1-silencing MDA-MB-231 cells.
L-Proline purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Oct 16;14(10):682. [Abstract]
Proline (0.8 mg/kg, i.p., 24 days) rescued the inhibitory effect of PYCR1 silencing on xenograft PY8119 tumor growth.
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Int J Pharm
The search for novel proline analogs for viscosity reduction and stabilization of highly concentrated monoclonal antibody solutions. [Abstract]2024 Apr 25:655:124055. PMID: 38554741 -
Molecules
Seeking Correlation Among Porin Permeabilities and Minimum Inhibitory Concentrations Through Machine Learning: A Promising Route to the Essential Molecular Descriptors. [Abstract]2025 Mar 9;30(6):1224. PMID: 40142001 -
Amino Acids
Identification of amino acids metabolomic profiling in human plasma distinguishes lupus nephritis from systemic lupus erythematosus. [Abstract]2024 Sep 18;56(1):56. PMID: 39292313 -
Biochem Biophys Res Commun
PYCR2 contributes to sunitinib resistance in hepatocellular carcinoma by activating the PPAR signaling pathway. [Abstract]2026 Jul 23:823:153971. PMID: 42155340 -
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Solvent & Solubility
H2O : 50 mg/mL (434.29 mM; Need ultrasonic)
DMSO : 2 mg/mL (17.37 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (868.58 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Li H, et al., l-Proline Alleviates Kidney Injury Caused by AFB1 and AFM1 through Regulating Excessive Apoptosis of Kidney Cells. Toxins (Basel). 2019 Apr 16;11(4):226. [Content Brief]
[2]. Washington JM, et al., L-Proline induces differentiation of ES cells: a novel role for an amino acid in the regulation of pluripotent cells in culture. Am J Physiol Cell Physiol. 2010 May;298(5):C982-92. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 8.6858 mL | 43.4292 mL | 86.8583 mL | 217.1458 mL |
| 5 mM | 1.7372 mL | 8.6858 mL | 17.3717 mL | 43.4292 mL | |
| 10 mM | 0.8686 mL | 4.3429 mL | 8.6858 mL | 21.7146 mL | |
| 15 mM | 0.5791 mL | 2.8953 mL | 5.7906 mL | 14.4764 mL | |
| H2O | 20 mM | 0.4343 mL | 2.1715 mL | 4.3429 mL | 10.8573 mL |
| 25 mM | 0.3474 mL | 1.7372 mL | 3.4743 mL | 8.6858 mL | |
| 30 mM | 0.2895 mL | 1.4476 mL | 2.8953 mL | 7.2382 mL | |
| 40 mM | 0.2171 mL | 1.0857 mL | 2.1715 mL | 5.4286 mL | |
| 50 mM | 0.1737 mL | 0.8686 mL | 1.7372 mL | 4.3429 mL | |
| 60 mM | 0.1448 mL | 0.7238 mL | 1.4476 mL | 3.6191 mL | |
| 80 mM | 0.1086 mL | 0.5429 mL | 1.0857 mL | 2.7143 mL | |
| 100 mM | 0.0869 mL | 0.4343 mL | 0.8686 mL | 2.1715 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L-Proline Related Classifications
HY-Y0252 Related Classifications
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