Cat. No.: HY-13694

Methionine

(Synonyms: MRX-1024; D-Methionine)

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

Methionine (MRX-1024; D-Methionine) is an effective chemoprotective agent which can also inhibit the neuronal activity through GABA_A receptor activation.

For research use only. We do not sell to patients.

Purity: 99.92%
CAS No.: 348-67-4
Formula: C₅H₁₁NO₂S
Molecular Weight:149.22

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Methionine Chemical Structure

CAS No.: 348-67-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Methionine (MRX-1024; D-Methionine) is an effective chemoprotective agent which can also inhibit the neuronal activity through GABA_A receptor activation.

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

The incubation of human plasma with Methionine (D-methionine) and CP leads to the formation of a Pt-D-methionine complex independent of the order of addition. In plasma, an early CP hydrolysis product reacts with Methionine to form a 1:1 complex that is followed by the formation of a 2:1 compound at a later time point. The formation of these Pt-D-methionine species plays an important role in the processes by which Methionine protects mammalian organisms against CP-induced toxicities^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

When Methionine (D-methionine) is administered along with cisplatin the cell density is 0.8±0.070 (SEM), significantly larger than in rats treated with only cisplatin (P<0.01) while not significantly different from controls or from animals treated only with D-methionine (P>0.05). When Methionine is administered alone the average cell density is 0.95±0.099 (SEM) and not significantly different from controls (P>0.05)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 348-67-4
Appearance Solid
Molecular Weight 149.22
Formula C₅H₁₁NO₂S
Color White to off-white
SMILES

N[C@H](CCSC)C(O)=O
Synonyms

MRX-1024; D-Methionine
Structure Classification
- Amino acids
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (7)

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Nat Metab

Extracellular acidosis restricts one-carbon metabolism and preserves T cell stemness. [Abstract]

2023 Feb;5(2):314-330. PMID: 36717749

Methionine purchased from MedChemExpress. Usage Cited in: Nat Metab. 2023 Feb;5(2):314-330. [Abstract]

Methionine supplementation to T cells under ↑[H+] exposure restored the total level of H3K27me3 expression in human T cells. H3K4me3, histone H3 trimethylated at K4; H3K79me2, H3 dimethylated at K79; H3K27me3, H3 trimethylated at K27; H3K9me2, H3 dimethylated at K9.

Methionine purchased from MedChemExpress. Usage Cited in: Nat Metab. 2023 Feb;5(2):314-330. [Abstract]

Left: Representative histogram of TCF1 in Ctrl (Methionine (Met; 100 μM)) and -Met (20 μM methionine) conditioned human CD8⁺ T cells. Right: The statistical percentage of TCF1⁺CD8⁺ T cells population.

Methionine purchased from MedChemExpress. Usage Cited in: Nat Metab. 2023 Feb;5(2):314-330. [Abstract]

Flow cytometric analysisof CD44⁺CD62L⁺ population in Ctrl (100 μM Methionine) and -Met (20 μM Methionine) conditioned T cells.

Methionine purchased from MedChemExpress. Usage Cited in: Nat Metab. 2023 Feb;5(2):314-330. [Abstract]

The phenotype of T cell stemness induced by extracellular acidosis was indeed partially prohibited by supplementation with Methionine. Representative FACS plots show percentage of CCR7⁺ CD62L⁺population in human CD8⁺ T cells cultured with the indicated conditions.

Methionine purchased from MedChemExpress. Usage Cited in: Nat Metab. 2023 Feb;5(2):314-330. [Abstract]

Representative histogram and quantification of TCF1 in human CD8⁺ T cells cultured with control, pH 6.6 or pH 6.6 supplemented with Methionine conditions.
Cell Death Discov

Metabolic interplay between exogenous cystine and glutamine dependence in triple-negative breast cancer. [Abstract]

2025 Oct 6;11(1):430. PMID: 41053011
Mater Des

2026 Apr 19;266:116048.
J Proteome Res

Distinguish Esophageal Cancer Cells through VOCs Induced by Methionine Regulation. [Abstract]

2024 Jul 5;23(7):2552-2560. PMID: 38864484
Amino Acids

Identification of amino acids metabolomic profiling in human plasma distinguishes lupus nephritis from systemic lupus erythematosus. [Abstract]

2024 Sep 18;56(1):56. PMID: 39292313
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Physiol Rep

Metabolic shifts in plasma amino acids and related metabolites in response to SGLT2 inhibition and hyperglycemia in type 1 diabetes. [Abstract]

2025 Aug;13(16):e70465. PMID: 40842430

Solvent & Solubility

In Vitro:

H₂O : 25 mg/mL (167.54 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.7015 mL

33.5076 mL

67.0151 mL

5 mM

1.3403 mL

6.7015 mL

13.4030 mL

10 mM

0.6702 mL

3.3508 mL

6.7015 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 16.67 mg/mL (111.71 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

Protocol

Cell Assay^[2]

Plasma (2.0 mL) is spiked with 81 mL of the CP stock solution, incubated at 37°C for 30 min and then 20 mL of theMethionine (D-methionine) stock solution (40.7 mg/mL) is added. The obtained mixture is analyzed after 10 min and 50 min of incubation at 37°C^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[3]

Three sham controls receive 0.9% saline instead of cisplatin. Three rats receive only cisplatin. Another three rats receive Methionine (D-methionine) 30 minutes prior to treatment with cisplatin. Injection of Methionine prior to cisplatin treatment ensures that it is already taken up before cisplatin is applied. Finally, three rats are treated only with Methionine. All 12 rats are sacrificed at day 7 post-treatment. For prescreening, two further rats are treated with cisplatin alone and sacrificed 2 or 21 days later. All rats are hydrated with 0.9% saline^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wu C, et al. Antioxidants L-carnitine and D-methionine modulate neuronal activity through GABAergic inhibition. J Neural Transm (Vienna). 2014 Jul;121(7):683-93. [Content Brief]

[2]. Sooriyaarachchi M, et al. Chemoprotection by D-methionine against cisplatin-induced side-effects: insight from in vitrostudies using human plasma. Metallomics. 2014 Mar;6(3):532-41. [Content Brief]

[3]. Hinduja S, et al. D-methionine protects against cisplatin-induced neurotoxicity in the hippocampus of the adult rat. Neurotox Res. 2015 Apr;27(3):199-204. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	6.7015 mL	33.5076 mL	67.0151 mL	167.5379 mL
	5 mM	1.3403 mL	6.7015 mL	13.4030 mL	33.5076 mL
	10 mM	0.6702 mL	3.3508 mL	6.7015 mL	16.7538 mL
	15 mM	0.4468 mL	2.2338 mL	4.4677 mL	11.1692 mL
	20 mM	0.3351 mL	1.6754 mL	3.3508 mL	8.3769 mL
	25 mM	0.2681 mL	1.3403 mL	2.6806 mL	6.7015 mL
	30 mM	0.2234 mL	1.1169 mL	2.2338 mL	5.5846 mL
	40 mM	0.1675 mL	0.8377 mL	1.6754 mL	4.1884 mL
	50 mM	0.1340 mL	0.6702 mL	1.3403 mL	3.3508 mL
	60 mM	0.1117 mL	0.5585 mL	1.1169 mL	2.7923 mL
	80 mM	0.0838 mL	0.4188 mL	0.8377 mL	2.0942 mL
	100 mM	0.0670 mL	0.3351 mL	0.6702 mL	1.6754 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Methionine

Immunology/Inflammation Compound Library

Membrane Transporter/Ion Channel Compound Library

Metabolism/Protease Compound Library

Neuronal Signaling Compound Library

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Publications Citing Use of MedChemExpress (MCE) Methionine

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (7)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Methionine Related Classifications

HY-13694 Related Classifications