2. Membrane Transporter/Ion Channel Neuronal Signaling Metabolic Enzyme/Protease
  3. GABA Receptor Endogenous Metabolite
  4. Methionine

Methionine  (Synonyms: MRX-1024; D-Methionine)

Cat. No.: HY-13694 Purity: 99.91%
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Methionine (MRX-1024; D-Methionine) is an effective chemoprotective agent which can also inhibit the neuronal activity through GABAA receptor activation.

For research use only. We do not sell to patients.

CAS No. : 348-67-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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10 mM * 1 mL in Water In-stock
Solid
1 g In-stock
5 g In-stock
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of Methionine:

Methionine Related Antibodies

Top Publications Citing Use of Products

    Methionine purchased from MedChemExpress. Usage Cited in: Nat Metab. 2023 Feb;5(2):314-330.  [Abstract]

    Methionine supplementation to T cells under ↑[H+] exposure restored the total level of H3K27me3 expression in human T cells. H3K4me3, histone H3 trimethylated at K4; H3K79me2, H3 dimethylated at K79; H3K27me3, H3 trimethylated at K27; H3K9me2, H3 dimethylated at K9.

    Methionine purchased from MedChemExpress. Usage Cited in: Nat Metab. 2023 Feb;5(2):314-330.  [Abstract]

    Left: Representative histogram of TCF1 in Ctrl (Methionine (Met; 100 μM)) and -Met (20 μM methionine) conditioned human CD8+ T cells. Right: The statistical percentage of TCF1+CD8+ T cells population.

    Methionine purchased from MedChemExpress. Usage Cited in: Nat Metab. 2023 Feb;5(2):314-330.  [Abstract]

    Flow cytometric analysisof CD44+CD62L+ population in Ctrl (100 μM Methionine) and -Met (20 μM Methionine) conditioned T cells.

    Methionine purchased from MedChemExpress. Usage Cited in: Nat Metab. 2023 Feb;5(2):314-330.  [Abstract]

    The phenotype of T cell stemness induced by extracellular acidosis was indeed partially prohibited by supplementation with Methionine. Representative FACS plots show percentage of CCR7+ CD62L+population in human CD8+ T cells cultured with the indicated conditions.

    Methionine purchased from MedChemExpress. Usage Cited in: Nat Metab. 2023 Feb;5(2):314-330.  [Abstract]

    Representative histogram and quantification of TCF1 in human CD8+ T cells cultured with control, pH 6.6 or pH 6.6 supplemented with Methionine conditions.

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Methionine (MRX-1024; D-Methionine) is an effective chemoprotective agent which can also inhibit the neuronal activity through GABAA receptor activation.

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    The incubation of human plasma with Methionine (D-methionine) and CP leads to the formation of a Pt-D-methionine complex independent of the order of addition. In plasma, an early CP hydrolysis product reacts with Methionine to form a 1:1 complex that is followed by the formation of a 2:1 compound at a later time point. The formation of these Pt-D-methionine species plays an important role in the processes by which Methionine protects mammalian organisms against CP-induced toxicities[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Methionine Related Antibodies
    In Vivo

    When Methionine (D-methionine) is administered along with cisplatin the cell density is 0.8±0.070 (SEM), significantly larger than in rats treated with only cisplatin (P<0.01) while not significantly different from controls or from animals treated only with D-methionine (P>0.05). When Methionine is administered alone the average cell density is 0.95±0.099 (SEM) and not significantly different from controls (P>0.05)[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    149.22

    Formula

    C5H11NO2S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    N[C@H](CCSC)C(O)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 25 mg/mL (167.54 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.7015 mL 33.5076 mL 67.0151 mL
    5 mM 1.3403 mL 6.7015 mL 13.4030 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 16.67 mg/mL (111.71 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.91%

    SDS (393 KB)

    COA (244 KB) HNMR (236 KB) RP-HPLC (275 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    Plasma (2.0 mL) is spiked with 81 mL of the CP stock solution, incubated at 37°C for 30 min and then 20 mL of theMethionine (D-methionine) stock solution (40.7 mg/mL) is added. The obtained mixture is analyzed after 10 min and 50 min of incubation at 37°C[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [3]

    Three sham controls receive 0.9% saline instead of cisplatin. Three rats receive only cisplatin. Another three rats receive Methionine (D-methionine) 30 minutes prior to treatment with cisplatin. Injection of Methionine prior to cisplatin treatment ensures that it is already taken up before cisplatin is applied. Finally, three rats are treated only with Methionine. All 12 rats are sacrificed at day 7 post-treatment. For prescreening, two further rats are treated with cisplatin alone and sacrificed 2 or 21 days later. All rats are hydrated with 0.9% saline[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 6.7015 mL 33.5076 mL 67.0151 mL 167.5379 mL
    5 mM 1.3403 mL 6.7015 mL 13.4030 mL 33.5076 mL
    10 mM 0.6702 mL 3.3508 mL 6.7015 mL 16.7538 mL
    15 mM 0.4468 mL 2.2338 mL 4.4677 mL 11.1692 mL
    20 mM 0.3351 mL 1.6754 mL 3.3508 mL 8.3769 mL
    25 mM 0.2681 mL 1.3403 mL 2.6806 mL 6.7015 mL
    30 mM 0.2234 mL 1.1169 mL 2.2338 mL 5.5846 mL
    40 mM 0.1675 mL 0.8377 mL 1.6754 mL 4.1884 mL
    50 mM 0.1340 mL 0.6702 mL 1.3403 mL 3.3508 mL
    60 mM 0.1117 mL 0.5585 mL 1.1169 mL 2.7923 mL
    80 mM 0.0838 mL 0.4188 mL 0.8377 mL 2.0942 mL
    100 mM 0.0670 mL 0.3351 mL 0.6702 mL 1.6754 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Methionine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Methionine348-67-4MRX-1024 D-MethionineMRX1024MRX 1024GABA ReceptorEndogenous MetaboliteGamma-aminobutyric acid Receptorγ-Aminobutyric acid ReceptorInhibitorinhibitorinhibit

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