Filipin III
Based on 24 publication(s) in Google Scholar
Filipin III is the major component of Filipin, a 28-membered ring pentaene macrolide antifungal antibiotic produced by S. filipinensis, S. avermitilis and S. miharaensis. Filipin interacts with membrane sterols causing the alteration of membrane structure.
For research use only. We do not sell to patients.
- Purity: 98.39%
- CAS No.: 480-49-9
- Formula: C35H58O11
- Molecular Weight:654.83
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Filipin III
More- BMEmat. 2026 Feb 11.
- Acta Pharm Sin B. 2025 Nov 13.
- Acta Pharm Sin B. 2025 Oct;15(10):5431-5443. [Abstract]
- Adv Sci (Weinh). 2025 May;12(17):e2417312. [Abstract]
- Chem Eng J. 2025 Jul 15.
- Exp Mol Med. 2025 May;57(5):1089-1105. [Abstract]
- J Exp Clin Cancer Res. 2025 Jul 26;44(1):220. [Abstract]
- Sci Adv. 2026 Mar 6;12(10):eadw0891. [Abstract]
- Int J Oral Sci. 2026 Jan 16;18(1):10. [Abstract]
- Mater Today Bio. 2025 Apr 17:32:101778. [Abstract]
- Int J Biol Sci. 2024 Jul 15;20(10):3942-3955. [Abstract]
- VIEW. 2025 Mar 23.
- Free Radic Biol Med. 2026 Mar 16:246:181-195. [Abstract]
- Oncogene. 2026 Apr;45(15):1386-1397. [Abstract]
- J Nutr Biochem. 2025 Dec 13:150:110231. [Abstract]
- Int J Mol Sci. 2025 Mar 29;26(7):3172. [Abstract]
- J Drug Deliv Sci Technol. 29 October 2022, 103925.
- Cell Oncol (Dordr). 2024 Dec;47(6):2147-2162. [Abstract]
- RSC Adv. 2026 Jan 28;16(7):5941-5955. [Abstract]
- Mol Pharm. 2024 May 9. [Abstract]
- Vet Microbiol. 2025 Apr 8:305:110507. [Abstract]
- Biochem Biophys Res Commun. 2025 Nov 25:794:153046. [Abstract]
- Npj Viruses. 2026 Feb 16;4(1):10. [Abstract]
- bioRxiv. 2023 Nov 4.
Customer Validation & Images
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Flow Cytometry
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Cell Imaging/Staining
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Cell Imaging/Staining
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
All Antibiotic Isoforms
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Biological Activity
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Macrolide |
Cell staining:
Prepare Filipin III working solution in TBS-T. NCI-60 cancer cells were treated with 50 μg/mL Filipin III in the dark. After incubation for 1 hour at room temperature, cells were washed with TBS-T. Coverslips were then detached and mounted in a water-based mountant. Images were taken on an Olympus Provis fluorescence microscope equipped with a 40X oil objective, and quantification of cholesterol levels was accomplished using the particle size algorithm in MetaXpress image analysis software (Molecular Devices Corp., Sunnyvale CA)[2].
Huang Y, et al assessed cholesterol enrichment on transsomes relative to TSPAN-GFP. NRK cells stably expressing TSPAN4-GFP were incubated with 2 μg/mL Filipin III at 37 °C. After 15 minutes of treatment, Z-stack images were acquired using confocal microscopy to measure filipin III intensity on migrasomes (IpM), TSPAN4-GFP intensity on migrasomes (ItM), filipin III intensity on RFs (IpR), and TSPAN4- GFP on RF. Strength(ItR)[3].
Or as Kwiatkowska K, et al exposed fibroblasts (Fibroblast) to 25 μg/ml Filipin III and incubated in the dark for 45 minutes (20 °C), the staining results were obtained[4].
Tissue staining:
Filipin binds to cholesterol with high affinity; however, it exhibits rapid photobleaching and produces only modest natural fluorescence upon UV excitation, making its detection under confocal microscopy difficult[5].
Reid PC et al used Filipin III to detect cholesterol accumulation in neurons. NPC cerebellar tissue was stained with Filipin III (125 μg/ml) for 2 h at room temperature in the dark. After incubation together, slides were washed with 0.5 M Tris without FBS and counterstained with fluorescent dyes. On day 22, cholesterol accumulation was observed in Purkinje neuronal cells. Or staining the NPC cortex with Filipin III, cholesterol accumulation in the axon hillock region of most neurons was observed on day 22[5].
Novak A et al use Filipin III to detect cholesterol accumulation in tumors. Dissolve Filipin III in DMSO/PBS (1:4) to make a working solution of 50 μg/mL. Subsequently, the tissue sections were incubated at room temperature for 30 min. After rinsing, the slides were mounted in an aqueous fluorescent mountant for subsequent observation[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 480-49-9
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Appearance Solid
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Molecular Weight 654.83
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Formula C35H58O11
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Color Light yellow to yellow
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SMILES
O=C1O[C@H](C)[C@@H](O)/C=C/C=C/C=C/C=C/C=C(C)/[C@@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)[C@H]1[C@H](O)CCCCC
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Structure Classification
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Initial Source
Streptomyces miharaensis
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (24)
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Journal Impact Factor
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Most Recent
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Filipin III purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Nov 13.
Uptake of Liposomes by bEnd.3 Cells. Cells were pre-incubated at 4℃ or treated with Filipin III (1 μg/mL) for 1 hour. DiI-labeled liposomes were then added to the culture wells and incubated for an additional 2 h. Cell fluorescence intensity was measured using flow cytometry.
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Acta Pharm Sin B
Spatiotemporally delivery of Cas9 ribonucleoprotein/DNAzyme logic systems using near-infrared upconversion nanomachine for precise immunotherapy. [Abstract]2025 Oct;15(10):5431-5443. PMID: 41132842 -
Adv Sci (Weinh)
Plac1+ Tumor Cell-Treg Interplay Supports Tumorigenesis and Progression of Head and Neck Cancer. [Abstract]2025 May;12(17):e2417312. PMID: 40056047 -
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Exp Mol Med
Cholesterol biosynthesis induced by radiotherapy inhibits cGAS-STING activation and contributes to colorectal cancer treatment resistance. [Abstract]2025 May;57(5):1089-1105. PMID: 40355720 -
J Exp Clin Cancer Res
Copy number amplification of TTPAL promotes cholesterol biosynthesis and esophageal squamous cell carcinoma progression via elevating NSUN2-mediated m5C modification of SREBP2 mRNA. [Abstract]2025 Jul 26;44(1):220. PMID: 40713894
Filipin III purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Jul 26;44(1):220. [Abstract]
Filipin III (0.05 mg/mL; 2 h). Filipin III staining displaying the cellular total cholesterol level in KYSE180 and KYSE30 cells with TTPAL depletion.
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Sci Adv
A worm-like nucleic acid nanostructure for gene delivery and endosomal escape via ClC3 ion exchanger. [Abstract]2026 Mar 6;12(10):eadw0891. PMID: 41790895 -
Int J Oral Sci
Antisense oligonucleotide targeting TARDBP-EGFR splicing axis inhibits progression of oral squamous cell carcinoma through ABCA1-regulated cholesterol efflux. [Abstract]2026 Jan 16;18(1):10. PMID: 41540015 -
Mater Today Bio
Membrane biomimetic nanoenzyme-incorporated hybrid glycyrrhizic acid hydrogel for precise mitochondrial ROS scavenging for osteoarthritis treatment. [Abstract]2025 Apr 17:32:101778. PMID: 40290887 -
Int J Biol Sci
Inhibition of PCSK9 enhances the anti-hepatocellular carcinoma effects of TCR-T cells and anti-PD-1 immunotherapy. [Abstract]2024 Jul 15;20(10):3942-3955. PMID: 39113701 -
Filipin III purchased from MedChemExpress. Usage Cited in: VIEW. 2025 Mar 23.
Filipin III (100 µg/mL; 3 h). The Filipin III staining of BT549 and DU145 cells transduced with shSCR or shSOAT1.
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Free Radic Biol Med
Endothelial JMJD1C drives pathological ocular neovascularization by activating SREBF2-dependent cholesterol biosynthesis. [Abstract]2026 Mar 16:246:181-195. PMID: 41548765 -
Oncogene
The NPC1/USP7/p53 axis regulates cholesterol and promotes the proliferation of hepatocellular carcinoma. [Abstract]2026 Apr;45(15):1386-1397. PMID: 41882104 -
J Nutr Biochem
Jujuboside A ameliorates glomerular podocytes lipotoxicity in diabetic mice by YY1-mediated promotion of intracellular cholesterol transport and efflux. [Abstract]2025 Dec 13:150:110231. PMID: 41397601 -
Int J Mol Sci
D-M159 Synergistically Induces Apoptosis in HeLa Cells Through Endoplasmic Reticulum Stress and Mitochondrial Dysfunction. [Abstract]2025 Mar 29;26(7):3172. PMID: 40243937
Filipin III purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 29;26(7):3172. [Abstract]
The pathways of D-M159 entry into cells were determined using 5 µg/mL Filipin III.
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Cell Oncol (Dordr)
Tumour cell-released autophagosomes promote lung metastasis by upregulating PD-L1 expression in pulmonary vascular endothelial cells in breast cancer. [Abstract]2024 Dec;47(6):2147-2162. PMID: 39373859 -
RSC Adv
Low-concentration cholesterol modification enhances Clematis filamentosa Dunn-derived extracellular vesicle-mediated macrophage polarization regulation for acute lung injury therapy. [Abstract]2026 Jan 28;16(7):5941-5955. PMID: 41613243 -
Mol Pharm
Saponin and Ribosome-Inactivating Protein Synergistically Trigger Lysosome-Dependent Apoptosis by Inhibiting Lysophagy: Potential to Become a New Antitumor Strategy. [Abstract]2024 May 9. PMID: 38722865
Filipin III purchased from MedChemExpress. Usage Cited in: Mol Pharm. 2024 May 9. [Abstract]
Fluorescence detection of cholesterol. HeLa cells were incubated with different concentrations of QS-21 (2.5, 5, and 7.5 μM) for 12 h, followed by staining with Filipin III (30 min). Images were captured by fluorescence microscopy.
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Vet Microbiol
Cholesterol-dependent Nsp5-endosomes co-trafficking to lysosomes facilitates porcine reproductive and respiratory syndrome virus replication by activating autophagy. [Abstract]2025 Apr 8:305:110507. PMID: 40215803 -
Biochem Biophys Res Commun
SBF-1 suppresses colorectal cancer cell growth via modulating cholesterol metabolic reprogramming. [Abstract]2025 Nov 25:794:153046. PMID: 41317708 -
Npj Viruses
STAT1 signaling controls cholesterol metabolism in epithelial cells and RSV-induced syncytia formation. [Abstract]2026 Feb 16;4(1):10. PMID: 41699197 -
Solvent & Solubility
DMSO : ≥ 10 mg/mL (15.27 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Methanol : 10 mg/mL (15.27 mM; Need ultrasonic and warming)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (280 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Payero TD, et al. Functional analysis of filipin tailoring genes from Streptomyces filipinensis reveals alternative routes in filipin III biosynthesis and yields bioactive derivatives. Microb Cell Fact. 2015 Aug 7;14:114. [Content Brief]
[2]. Warita K, et al. Statin-induced mevalonate pathway inhibition attenuates the growth of mesenchymal-like cancer cells that lack functional E-cadherin mediated cell cohesion. Sci Rep. 2014 Dec 23;4:7593. [Content Brief]
[3]. Huang Y, et al. Migrasome formation is mediated by assembly of micron-scale tetraspanin macrodomains. Nat Cell Biol. 2019 Aug;21(8):991-1002. [Content Brief]
[4]. Kwiatkowska K, et al. Visualization of cholesterol deposits in lysosomes of Niemann-Pick type C fibroblasts using recombinant perfringolysin O. Orphanet J Rare Dis. 2014 Apr 28;9:64. [Content Brief]
[5]. Reid PC, et al. A novel cholesterol stain reveals early neuronal cholesterol accumulation in the Niemann-Pick type C1 mouse brain. J Lipid Res. 2004 Mar;45(3):582-91. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Methanol | 1 mM | 1.5271 mL | 7.6356 mL | 15.2711 mL | 38.1778 mL |
| 5 mM | 0.3054 mL | 1.5271 mL | 3.0542 mL | 7.6356 mL | |
| 10 mM | 0.1527 mL | 0.7636 mL | 1.5271 mL | 3.8178 mL | |
| 15 mM | 0.1018 mL | 0.5090 mL | 1.0181 mL | 2.5452 mL |
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