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製品番号: HY-186291

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A-486

A-486 is an inactive analog that serves as an inactive control analog for A-485 (HY-107455).

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 2143464-15-5
分子式: C₂₅H₂₄F₄N₄O₅
分子量:536.48

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

A-486 構造式

CAS 番号: 2143464-15-5

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

A-486 is an inactive analog that serves as an inactive control analog for A-485 (HY-107455)^[1].

体外実験

A-486 shows very weak inhibitory activity against purified p300-BHC HAT domain in vitro, with an IC₅₀ greater than 10,000 nM^[1].
A-486 shows very weak inhibitory activity against purified CBP-BHC HAT domain in vitro, with an IC₅₀ of 9,032 nM^[1].
A-486 (dose range) binds very weakly to purified p300 HAT domain in vitro, with a K_d of 20,000 nM and a fast off-rate^[1].
A-486 (3 h) is inactive in PC-3 prostate adenocarcinoma cells, failing to reduce H3K27Ac or H3K9Ac levels after 3 hours of treatment^[1].
A-486 (10 μM; 7-24 h) does not inhibit DHT-stimulated PSA mRNA expression in LnCaP-FGC prostate cancer cells after 7 or 24 hours of treatment^[1].
A-486 (10 μM; 7-24 h) does not inhibit MYC mRNA expression in androgen-depleted 22Rv1 prostate cancer cells after 7 or 24 hours of treatment^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .

Real Time qPCR^[1]

View Protocol

Cell Line:

LnCaP-FGC androgen-dependent prostate adenocarcinoma cells
Concentration:

10 μM
Incubation Time:

7 h; 24 h
Result:

Did not inhibit DHT-stimulated PSA mRNA expression, remaining at levels comparable to the DMSO control at both time points.

Real Time qPCR^[1]

View Protocol

Cell Line:

androgen-depleted 22Rv1 castration-resistant prostate adenocarcinoma cells
Concentration:

10 μM
Incubation Time:

7 h; 24 h
Result:

Did not reduce MYC mRNA expression, remaining at levels comparable to the DMSO control at both time points.

化学情報

CAS 番号 2143464-15-5
分子量 536.48
分子式 C₂₅H₂₄F₄N₄O₅
SMILES

O=C1[C@]2(OC(N1CC(N([C@@H](C)C(F)(F)F)CC3=CC=C(C=C3)F)=O)=O)C4=CC(NC(NC)=O)=CC=C4CC2
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Lasko LM, et al. Discovery of a selective catalytic p300/CBP inhibitor that targets lineage-specific tumours. Nature. 2017 Oct 05;550(7674):128-132. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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希釈計算機

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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