PA22-2 TFA
Based on 1 Customer Validation
PA22-2 (IKVAV sequence; Laminin A-chain fragment) TFA is a laminin receptor ligand. PA22-2 TFA competes with laminin for receptor binding sites, thereby mediating biological responses such as cell adhesion, migration, morphology regulation and angiogenesis. PA22-2 TFA promotes adhesion, spreading, directional migration and axon growth of specific cells, and supports endothelial cell mobilization and vascular formation. PA22-2 TFA inhibits laminin-mediated adhesion and migration in melanoma cells, and its antiserum reacts with the laminin A chain and native laminin, effectively blocking cell adhesion and axon growth.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 95.77%
- 分子式: C82H150N32O25S·xC2HF3O2
- 分子量:2016.33 (free base)
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
PA22-2 TFA (200 μg; 2 h coverslip coating, 18 h cell incubation) induces a fibroblastoid, migratory morphology in human umbilical vein endothelial cells (HUVEC), with most cells losing contact inhibition and developing extended cellular processes[2].
PA22-2 TFA (200 μg/mL; 6 h) induces a threefold increase in type IV collagenase activity, including active forms at 68 Kd and 62 Kd, in human umbilical vein endothelial cells (HUVEC) on Matrigel, leading to degradation of the Matrigel matrix and release of collagen IV fragments[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
PA22-2 TFA (200 μg; topical application via coated coverslip) significantly stimulates angiogenesis in the chick chorioallantoic membrane assay, increasing mean vessel area relative to untreated controls[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Fertilized eggs, 4-day-old[2]
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Dosage:2 mg/mL
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Administration:topical application to yolk sac membrane
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Result:Stimulated angiogenesis, increasing vessel thickness, abundance, and branching in 27 out of 30 eggs tested.
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Animal Model:Fertilized eggs, 9-day-old[2]
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Dosage:200 μg
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Administration:topical application via coated coverslip
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Result:Increased the mean vessel area to 4.2, with a statistically significant P-value of 0.019 compared to no treatment.
化学情報
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性状 Solid
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分子量 2016.33 (free base)
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分子式 C82H150N32O25S·xC2HF3O2
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SMILES
O=C(N[C@@H](CO)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCCN)C(N[C@@H](CCC(N)=O)C(N[C@@H](C)C(N[C@@H](C)C(N[C@@H](CO)C(N[C@@H]([C@@H](C)CC)C(N[C@@H](CCCCN)C(N[C@@H](C(C)C)C(N[C@@H](C)C(N[C@@H](C(C)C)C(N[C@@H](CO)C(N[C@@H](C)C(N[C@@H](CC(O)=O)C(N[C@@H](CCCNC(N)=N)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)[C@H](CS)N.OC(C(F)(F)F)=O.[x]
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別名
IKVAV sequence TFA; Laminin A-chain fragment TFA
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配列
Cys-Ser-Arg-Ala-Arg-Lys-Gln-Ala-Ala-Ser-Ile-Lys-Val-Ala-Val-Ser-Ala-Asp-Arg-NH2
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シーケンスの短縮
CSRARKQAASIKVAVSADR-NH2
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
H2O : 50 mg/mL (Need ultrasonic)
純度とドキュメンテーション
参考文献
[1]. Tashiro K, et al. A synthetic peptide containing the IKVAV sequence from the A chain of laminin mediates cell attachment, migration, and neurite outgrowth. The Journal of biological chemistry. 1989 Sep 25;264(27):16174-82. [Content Brief]
[2]. Grant DS, et al. Interaction of endothelial cells with a laminin A chain peptide (SIKVAV) in vitro and induction of angiogenic behavior in vivo. Journal of cellular physiology. 1992 Dec;153(3):614-25. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
PA22-2 TFA 関連分類
HY-P5353B 関連分類
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