country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-P5353B

Copy Link Print Information

PA22-2 TFA

(Synonyms: IKVAV sequence TFA; Laminin A-chain fragment TFA)

고객리뷰

Based on 1 Customer Validation

PA22-2 (IKVAV sequence; Laminin A-chain fragment) TFA is a laminin receptor ligand. PA22-2 TFA competes with laminin for receptor binding sites, thereby mediating biological responses such as cell adhesion, migration, morphology regulation and angiogenesis. PA22-2 TFA promotes adhesion, spreading, directional migration and axon growth of specific cells, and supports endothelial cell mobilization and vascular formation. PA22-2 TFA inhibits laminin-mediated adhesion and migration in melanoma cells, and its antiserum reacts with the laminin A chain and native laminin, effectively blocking cell adhesion and axon growth.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 95.77%
화학식: C₈₂H₁₅₀N₃₂O₂₅S·xC₂HF₃O₂
분자량:2016.33 (free base)

보관:

Sealed storage, away from moisture.

Powder -80°C, 2 years , -20°C, 1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

PA22-2 TFA Chemical Structure

사이즈

가격

재고

수량

Amount: USD 0.00

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- In Vitro
- In Vivo
Chemical Information
용액&용해도
- In Vitro
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

In Vitro

PA22-2 TFA (200 μg; 2 h coverslip coating, 18 h cell incubation) induces a fibroblastoid, migratory morphology in human umbilical vein endothelial cells (HUVEC), with most cells losing contact inhibition and developing extended cellular processes^[2].
PA22-2 TFA (200 μg/mL; 6 h) induces a threefold increase in type IV collagenase activity, including active forms at 68 K_d and 62 K_d, in human umbilical vein endothelial cells (HUVEC) on Matrigel, leading to degradation of the Matrigel matrix and release of collagen IV fragments^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

PA22-2 TFA (2 mg/mL; topical application to yolk sac membrane) stimulates angiogenesis in the chick yolk sac membrane assay, promoting vessel thickness, abundance, and branching in 90% of tested eggs^[2].
PA22-2 TFA (200 μg; topical application via coated coverslip) significantly stimulates angiogenesis in the chick chorioallantoic membrane assay, increasing mean vessel area relative to untreated controls^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Fertilized eggs, 4-day-old^[2]
Dosage:

2 mg/mL
Administration:

topical application to yolk sac membrane
Result:

Stimulated angiogenesis, increasing vessel thickness, abundance, and branching in 27 out of 30 eggs tested.

Animal Model:

Fertilized eggs, 9-day-old^[2]
Dosage:

200 μg
Administration:

topical application via coated coverslip
Result:

Increased the mean vessel area to 4.2, with a statistically significant P-value of 0.019 compared to no treatment.

Chemical Information

Appearance Solid
분자량 2016.33 (free base)
화학식 C₈₂H₁₅₀N₃₂O₂₅S·xC₂HF₃O₂
SMILES

O=C(N[C@@H](CO)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCCN)C(N[C@@H](CCC(N)=O)C(N[C@@H](C)C(N[C@@H](C)C(N[C@@H](CO)C(N[C@@H]([C@@H](C)CC)C(N[C@@H](CCCCN)C(N[C@@H](C(C)C)C(N[C@@H](C)C(N[C@@H](C(C)C)C(N[C@@H](CO)C(N[C@@H](C)C(N[C@@H](CC(O)=O)C(N[C@@H](CCCNC(N)=N)C(N)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)[C@H](CS)N.OC(C(F)(F)F)=O.[x]
Synonyms

IKVAV sequence TFA; Laminin A-chain fragment TFA
Sequence

Cys-Ser-Arg-Ala-Arg-Lys-Gln-Ala-Ala-Ser-Ile-Lys-Val-Ala-Val-Ser-Ala-Asp-Arg-NH2
Sequence Shortening

CSRARKQAASIKVAVSADR-NH2
선적

Room temperature in continental US; may vary elsewhere.

보관

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

용액&용해도

In Vitro:

H₂O : 50 mg/mL (Need ultrasonic)

View the Complete Stock Solution Preparation Table

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tashiro K, et al. A synthetic peptide containing the IKVAV sequence from the A chain of laminin mediates cell attachment, migration, and neurite outgrowth. The Journal of biological chemistry. 1989 Sep 25;264(27):16174-82. [Content Brief]

[2]. Grant DS, et al. Interaction of endothelial cells with a laminin A chain peptide (SIKVAV) in vitro and induction of angiogenic behavior in vivo. Journal of cellular physiology. 1992 Dec;153(3):614-25. [Content Brief]

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

Minimum order quantity

100 mg

견적 받기 해외재고보유

Other size

견적 받기