L-Stepholidine
Based on 1 Customer Validation
L-Stepholidine (Stepholidine) is an alkaloid. L-Stepholidine exhibits mixed dopamine D1 receptor agonist and D2 antagonist properties. L-Stepholidine has neuroprotective effect. L-Stepholidine can be used for the researches of neurological disease, such as Parkinson disease, Alzheimer's disease and opiate addiction.
For research use only. We do not sell to patients.
- Purity: 99.41%
- CAS No.: 16562-13-3
- Formula: C19H21NO4
- Molecular Weight:327.37
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Dopamine Receptor Isoforms
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Biological Activity
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D1 Receptor |
D2 Receptor |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
105.41 nM
Compound: 8, l-SPD
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Displacement of [3H]Spiprone from human dopamine D2 receptor expressed in HEK293 cells
Displacement of [3H]Spiprone from human dopamine D2 receptor expressed in HEK293 cells
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[PMID: 22748706] |
| HEK293 | IC50 |
12.29 nM
Compound: 8, l-SPD
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Displacement of [3H]SCH23390 from human dopamine D1 receptor expressed in HEK293 cells
Displacement of [3H]SCH23390 from human dopamine D1 receptor expressed in HEK293 cells
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[PMID: 22748706] |
| THP-1 | IC50 |
104.58 nM
Compound: 8, l-SPD
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Inhibition of tissue factor procoagulant activity in LPS-stimulated human THP1 cells preincubated for 1 hr before LPS addition measured after 5 hrs
Inhibition of tissue factor procoagulant activity in LPS-stimulated human THP1 cells preincubated for 1 hr before LPS addition measured after 5 hrs
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[PMID: 23199480] |
L-Stepholidine (10 mg/kg, i.p., daily for 22 days) attenuates L-DOPA (HY-N0304)-induced dyskinesia (LID) in 6-hydroxydopamine (6-OHDA) -lesioned PD rats models[2].
L-Stepholidine (20 mg/kg, i.p., daily for 7-21 days) attenuates the maintenance of neurotransmitter-induced conditioned place preference (CPP) in rats[3].
L-Stepholidine (10-20 mg/kg, i.p., 30 mins before morphine injection) blocks neurotransmitter-induced re-acquisition of morphine CPP in rats[3].
L-Stepholidine (10 mg/kg, i.p.) rescues memory deficit and synaptic plasticity in APP/PS1 transgenic mice[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:6-hydroxydopamine (6-OHDA) -lesioned PD rats models treated with L-DOPA (HY-N0304)[2]
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Dosage:10 mg/kg
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Administration:Intraperitoneally injection, daily for 22 days
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Result:Displayed much less severe dyskinesia.
Attenuated L-DOPA-induced axial and limb dyskinesia.
Did not produce any significant axial, limb, and orolingual AIMs.
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Animal Model:APP/PS1 transgenic mice[4]
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Dosage:10 mg/kg
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Administration:Intraperitoneally injection
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Result:Improved impaired learning and memory.
Rescued the surface expression of GluA1-containing AMPA receptors and spine density in the hippocampus.
Chemical Information
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CAS No. 16562-13-3
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Appearance Solid
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Molecular Weight 327.37
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Formula C19H21NO4
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Color White to off-white
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SMILES
OC1=C(OC)C=C(CCN2CC3=C(OC)C(O)=CC=C3C[C@]24[H])C4=C1
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Synonyms
Stepholidine; (-)-Stepholidine; L-SPD
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (305.46 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Ma B, et al. L-stepholidine, a natural dopamine receptor D1 agonist and D2 antagonist, inhibits heroin-induced reinstatement. Neurosci Lett. 2014 Jan 24;559:67-71. [Content Brief]
[2]. Mo J, et al. L-stepholidine reduced L-DOPA-induced dyskinesia in 6-OHDA-lesioned rat model of Parkinson's disease. Neurobiol Aging. 2010 Jun;31(6):926-36. [Content Brief]
[3]. Wang W, et al. The effect of L-stepholidine, a novel extract of Chinese herb, on the acquisition, expression, maintenance, and re-acquisition of morphine conditioned place preference in rats. Neuropharmacology. 2007 Feb;52(2):355-61. [Content Brief]
[4]. Hao JR, et al. L-Stepholidine rescues memory deficit and synaptic plasticity in models of Alzheimer's disease via activating dopamine D1 receptor/PKA signaling pathway. Cell Death Dis. 2015 Nov 5;6(11):e1965. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0546 mL | 15.2732 mL | 30.5465 mL | 76.3662 mL |
| 5 mM | 0.6109 mL | 3.0546 mL | 6.1093 mL | 15.2732 mL | |
| 10 mM | 0.3055 mL | 1.5273 mL | 3.0546 mL | 7.6366 mL | |
| 15 mM | 0.2036 mL | 1.0182 mL | 2.0364 mL | 5.0911 mL | |
| 20 mM | 0.1527 mL | 0.7637 mL | 1.5273 mL | 3.8183 mL | |
| 25 mM | 0.1222 mL | 0.6109 mL | 1.2219 mL | 3.0546 mL | |
| 30 mM | 0.1018 mL | 0.5091 mL | 1.0182 mL | 2.5455 mL | |
| 40 mM | 0.0764 mL | 0.3818 mL | 0.7637 mL | 1.9092 mL | |
| 50 mM | 0.0611 mL | 0.3055 mL | 0.6109 mL | 1.5273 mL | |
| 60 mM | 0.0509 mL | 0.2546 mL | 0.5091 mL | 1.2728 mL | |
| 80 mM | 0.0382 mL | 0.1909 mL | 0.3818 mL | 0.9546 mL | |
| 100 mM | 0.0305 mL | 0.1527 mL | 0.3055 mL | 0.7637 mL |
L-Stepholidine Related Classifications
HY-N6960 Related Classifications
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