Cat. No.: HY-N4183

Licoflavone C

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Licoflavone C is a broad-spectrum antiviral inhibitor with estrogen-like properties. Licoflavone C binds to viral endonuclease (CEN) and inhibits the replication of various bunyaviruses including severe fever with thrombocytopenia syndrome virus (SFTSV) and lymphocytic choriomeningitis virus in a non-substrate competitive manner. The IC₅₀ values of Licoflavone C against SFTSV CEN and SFTSV CEN are 35.5 μM and 135.8 μM, respectively, and its K_d value against SFTSV CEN is 9.53 μM. After viral entry into cells, Licoflavone C reduces viral loads in mouse tissues in a dose-dependent manner, and exhibits extremely low cytotoxicity and genotoxicity. Licoflavone C induces apoptosis by increasing caspase 3/7 activity, blocks the cell cycle, and alleviates chemotherapy-induced chromosomal damage. Licoflavone C is applicable to the research on severe fever with thrombocytopenia syndrome and related viral infection mechanisms.

For research use only. We do not sell to patients.

Purity: 99.83%
CAS No.: 72357-31-4
Formula: C₂₀H₁₈O₅
Molecular Weight:338.35

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Licoflavone C Chemical Structure

CAS No.: 72357-31-4

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Parmacokinetics
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

Caspase 3

Caspase-7

All Caspase Isoforms :

All Estrogen Receptor/ERR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
COS-7	IC₅₀	1.29 μM Compound: 8-PA	Inhibition of human recombinant PDE5A1 expressed in COS7 cells Inhibition of human recombinant PDE5A1 expressed in COS7 cells	[PMID: 18778098]
HeLa	IC₅₀	>40 μM Compound: 2	Cytotoxicity against human HeLa cells assessed as inhibition of cell proliferation by MTT assay Cytotoxicity against human HeLa cells assessed as inhibition of cell proliferation by MTT assay	[PMID: 33609662]
HepG2	IC₅₀	>40 μM Compound: 2	Cytotoxicity against human HepG2 cells assessed as inhibition of cell proliferation by MTT assay Cytotoxicity against human HepG2 cells assessed as inhibition of cell proliferation by MTT assay	[PMID: 33609662]
MCF7	IC₅₀	>40 μM Compound: 2	Cytotoxicity against human MCF7 cells assessed as inhibition of cell proliferation by MTT assay Cytotoxicity against human MCF7 cells assessed as inhibition of cell proliferation by MTT assay	[PMID: 33609662]
NCI-H460	IC₅₀	>40 μM Compound: 2	Cytotoxicity against human NCI-H460 cells assessed as inhibition of cell proliferation by MTT assay Cytotoxicity against human NCI-H460 cells assessed as inhibition of cell proliferation by MTT assay	[PMID: 33609662]
RAW264.7	IC₅₀	3.83 μM Compound: 43	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess reagent based assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess reagent based assay	[PMID: 28522265]

In Vitro

Licoflavone C (50 μM; 0.5 h) potently inhibits the enzymatic activity of purified SFTSV CEN protein in vitro^[1].
Licoflavone C (15 μM) effectively inhibits the proliferation of SFTSV in Vero cells at the post-entry stage, with the strongest activity when administered within 12 h post-infection. Its EC₅₀ value is 1.90 μM for post-infection administration and 1.84 μM for full-course administration^[1].
Licoflavone C (incubated with SFTSV CEN for 30 min; 50-200 μM) inhibits SFTSV CEN-mediated RNA cleavage in a concentration- and time-dependent manner in vitro, with an IC₅₀ of 35.5 μM^[1].
Licoflavone C potently inhibits infections by HRTV, GTV, and LCMV in vitro, with EC₅₀ values of 9.21 μM, 4.21 μM, and 2.61 μM, respectively^[1].
Licoflavone C (0.1-300 μM; 48 h) does not induce chromosome damage in cultured human peripheral blood lymphocytes even at concentrations up to 300 μM, but it triggers dose-dependent cell cycle disturbances starting from 10 μM and exhibits strong toxicity at 600 μM^[2].
Licoflavone C (0.1-1.0 μM; 48 h) significantly reduces the chromosomal damage induced by Daunorubicin (HY-13062A) and Mitomycin C (HY-13316) in cultured human peripheral blood lymphocytes in vitro, but fails to ameliorate the cell cycle progression impairment caused by the mutagens^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[1]

Cell Line:

Vero cells infected with SFTSV
Concentration:

Gradient dilutions
Incubation Time:

1 h pre-incubation, then continuous treatment for 36 h total
Result:

Exhibited an EC₅₀ of 1.85 μM against SFTSV infection in Vero cells, with a CC₅₀ >300.0 μM and a selectivity index (SI) >162.16.

Real Time qPCR^[1]

Cell Line:

Vero cells infected with HRTV or GTV; BHK-21 cells infected with LCMV
Concentration:

Gradient dilutions
Incubation Time:

1 h pre-incubation, then continuous treatment for 36 h total
Result:

Exhibited EC₅₀ values of 9.21 μM against HRTV in Vero cells, 4.21 μM against GTV in Vero cells, and 2.61 μM against LCMV in BHK-21 cells.

Parmacokinetics

Species	Dose	Route	T_1/2	C_max	Bioavailability
Rat^[1]	20 mg/kg	i.v.	0.5±0.115 h	20500±2610 ng/mL	/
Rat^[1]	20 mg/kg	i.g.	5.23±0.84 h	14.7±18.4 ng/mL	0.5±0.09 %

In Vivo

Licoflavone C (20 mg/kg; intravenous injection; once daily for 3 consecutive days) significantly reduces SFTSV viral loads in the liver, spleen and blood of C57BL/6 J mice infected with SFTSV^[1].
Licoflavone C (5-20 mg/kg; intravenous injection; once every 12 hours; for 2 consecutive days) dose-dependently reduces the SFTSV viral load in the liver, spleen, kidney and blood of C57BL/6 J mice infected with SFTSV^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 J (female, 6-8 weeks of age, challenged with SFTSV via intraperitoneal injection)^[1]
Dosage:

20 mg/kg
Administration:

i.v.; once daily; 3 consecutive days
Result:

Reduced SFTSV viral loads in the liver, spleen, and blood significantly compared to vehicle control.
Showed no significant reduction in kidney viral loads.

Animal Model:

C57BL/6 J (female, 6-8 weeks of age, challenged with SFTSV via intraperitoneal injection)^[1]
Dosage:

5 mg/kg; 10 mg/kg; 20 mg/kg
Administration:

i.v.; every 12 hours; 2 days
Result:

Reduced SFTSV viral loads in the liver, spleen, kidney, and blood in a dose-dependent manner.
Reduced SFTSV viral loads across all four analyzed tissues significantly at 20 mg/kg.
Reduced SFTSV viral loads in the spleen, kidney, and blood significantly at 10 mg/kg.
Reduced SFTSV viral load in the spleen only significantly at 5 mg/kg.
Reduced SFTSV nucleoprotein fluorescence intensity in spleen tissue, consistent with viral load reductions.

Chemical Information

CAS No. 72357-31-4
Appearance Solid
Molecular Weight 338.35
Formula C₂₀H₁₈O₅
Color Light yellow to brown
SMILES

O=C1C=C(C2=CC=C(O)C=C2)OC3=C(C/C=C(C)\C)C(O)=CC(O)=C13
Structure Classification
- Flavonoids
- Flavones
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Journal Impact Factor

Food Res Int

Glycyrrhiza uralensis Fisch: A novel source of analgesic activity through NaV1.8 sodium channel modulation. [Abstract]

2025 Dec;222(Pt 1):117620. PMID: 41267240

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (184.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

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Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9555 mL

14.7776 mL

29.5552 mL

5 mM

0.5911 mL

2.9555 mL

5.9110 mL

10 mM

0.2956 mL

1.4778 mL

2.9555 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Gao X, et al. Identification of Licoflavone C as a cap-dependent endonuclease inhibitor against severe fever with thrombocytopenia syndrome virus. Acta Pharmacol Sin. 2025;46(9):2482-2495. [Content Brief]

[2]. Scarpato R, et al. Licoflavone C attenuates the genotoxicity of cancer drugs in human peripheral lymphocytes. Phytother Res. 2008;22(12):1650-1654. [Content Brief]

[3]. Zejli H, et al. Phytochemical analysis, antioxidant, analgesic, anti-inflammatory, hemagglutinin and hemolytic activities of chemically characterized extracts from Origanum grosii (L.) and Thymus pallidus (L.)[J]. Plants, 2024, 13(3): 385.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9555 mL	14.7776 mL	29.5552 mL	73.8880 mL
	5 mM	0.5911 mL	2.9555 mL	5.9110 mL	14.7776 mL
	10 mM	0.2956 mL	1.4778 mL	2.9555 mL	7.3888 mL
	15 mM	0.1970 mL	0.9852 mL	1.9703 mL	4.9259 mL
	20 mM	0.1478 mL	0.7389 mL	1.4778 mL	3.6944 mL
	25 mM	0.1182 mL	0.5911 mL	1.1822 mL	2.9555 mL
	30 mM	0.0985 mL	0.4926 mL	0.9852 mL	2.4629 mL
	40 mM	0.0739 mL	0.3694 mL	0.7389 mL	1.8472 mL
	50 mM	0.0591 mL	0.2956 mL	0.5911 mL	1.4778 mL
	60 mM	0.0493 mL	0.2463 mL	0.4926 mL	1.2315 mL
	80 mM	0.0369 mL	0.1847 mL	0.3694 mL	0.9236 mL
	100 mM	0.0296 mL	0.1478 mL	0.2956 mL	0.7389 mL

Keywords

Licoflavone C

1 Cited Publications

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Licoflavone C

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All Caspase Isoforms

All Estrogen Receptor/ERR Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Licoflavone C Related Classifications

Cat. No.: HY-N4183