2. Apoptosis Vitamin D Related/Nuclear Receptor
  3. Apoptosis Caspase Estrogen Receptor/ERR
  4. Licoflavone C

Licoflavone C is a broad-spectrum antiviral inhibitor with estrogen-like properties. Licoflavone C binds to viral endonuclease (CEN) and inhibits the replication of various bunyaviruses including severe fever with thrombocytopenia syndrome virus (SFTSV) and lymphocytic choriomeningitis virus in a non-substrate competitive manner. The IC50 values of Licoflavone C against SFTSV CEN and SFTSV CEN are 35.5 μM and 135.8 μM, respectively, and its Kd value against SFTSV CEN is 9.53 μM. After viral entry into cells, Licoflavone C reduces viral loads in mouse tissues in a dose-dependent manner, and exhibits extremely low cytotoxicity and genotoxicity. Licoflavone C induces apoptosis by increasing caspase 3/7 activity, blocks the cell cycle, and alleviates chemotherapy-induced chromosomal damage. Licoflavone C is applicable to the research on severe fever with thrombocytopenia syndrome and related viral infection mechanisms.

For research use only. We do not sell to patients.

Licoflavone C

Licoflavone C Chemical Structure

CAS No. : 72357-31-4

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Licoflavone C Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Licoflavone C

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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Estrogen receptor ERα ERβ ERRα ERRβ ERRγ

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Licoflavone C is a broad-spectrum antiviral inhibitor with estrogen-like properties. Licoflavone C binds to viral endonuclease (CEN) and inhibits the replication of various bunyaviruses including severe fever with thrombocytopenia syndrome virus (SFTSV) and lymphocytic choriomeningitis virus in a non-substrate competitive manner. The IC50 values of Licoflavone C against SFTSV CEN and SFTSV CEN are 35.5 μM and 135.8 μM, respectively, and its Kd value against SFTSV CEN is 9.53 μM. After viral entry into cells, Licoflavone C reduces viral loads in mouse tissues in a dose-dependent manner, and exhibits extremely low cytotoxicity and genotoxicity. Licoflavone C induces apoptosis by increasing caspase 3/7 activity, blocks the cell cycle, and alleviates chemotherapy-induced chromosomal damage. Licoflavone C is applicable to the research on severe fever with thrombocytopenia syndrome and related viral infection mechanisms[1][2][3].

IC50 & Target

Caspase 3

 

Caspase-7

 

Cellular Effect
Cell Line Type Value Description References
COS-7 IC50
1.29 μM
Compound: 8-PA
Inhibition of human recombinant PDE5A1 expressed in COS7 cells
Inhibition of human recombinant PDE5A1 expressed in COS7 cells
[PMID: 18778098]
HeLa IC50
> 40 μM
Compound: 2
Cytotoxicity against human HeLa cells assessed as inhibition of cell proliferation by MTT assay
Cytotoxicity against human HeLa cells assessed as inhibition of cell proliferation by MTT assay
[PMID: 33609662]
HepG2 IC50
> 40 μM
Compound: 2
Cytotoxicity against human HepG2 cells assessed as inhibition of cell proliferation by MTT assay
Cytotoxicity against human HepG2 cells assessed as inhibition of cell proliferation by MTT assay
[PMID: 33609662]
MCF7 IC50
> 40 μM
Compound: 2
Cytotoxicity against human MCF7 cells assessed as inhibition of cell proliferation by MTT assay
Cytotoxicity against human MCF7 cells assessed as inhibition of cell proliferation by MTT assay
[PMID: 33609662]
NCI-H460 IC50
> 40 μM
Compound: 2
Cytotoxicity against human NCI-H460 cells assessed as inhibition of cell proliferation by MTT assay
Cytotoxicity against human NCI-H460 cells assessed as inhibition of cell proliferation by MTT assay
[PMID: 33609662]
RAW264.7 IC50
3.83 μM
Compound: 43
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess reagent based assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess reagent based assay
[PMID: 28522265]
In Vitro

Licoflavone C (50 μM; 0.5 h) potently inhibits the enzymatic activity of purified SFTSV CEN protein in vitro[1].
Licoflavone C (15 μM) effectively inhibits the proliferation of SFTSV in Vero cells at the post-entry stage, with the strongest activity when administered within 12 h post-infection. Its EC50 value is 1.90 μM for post-infection administration and 1.84 μM for full-course administration[1].
Licoflavone C (incubated with SFTSV CEN for 30 min; 50-200 μM) inhibits SFTSV CEN-mediated RNA cleavage in a concentration- and time-dependent manner in vitro, with an IC50 of 35.5 μM[1].
Licoflavone C potently inhibits infections by HRTV, GTV, and LCMV in vitro, with EC50 values of 9.21 μM, 4.21 μM, and 2.61 μM, respectively[1].
Licoflavone C (0.1-300 μM; 48 h) does not induce chromosome damage in cultured human peripheral blood lymphocytes even at concentrations up to 300 μM, but it triggers dose-dependent cell cycle disturbances starting from 10 μM and exhibits strong toxicity at 600 μM[2].
Licoflavone C (0.1-1.0 μM; 48 h) significantly reduces the chromosomal damage induced by Daunorubicin (HY-13062A) and Mitomycin C (HY-13316) in cultured human peripheral blood lymphocytes in vitro, but fails to ameliorate the cell cycle progression impairment caused by the mutagens[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Licoflavone C Related Antibodies

Real Time qPCR[1]

Cell Line: Vero cells infected with SFTSV
Concentration: Gradient dilutions
Incubation Time: 1 h pre-incubation, then continuous treatment for 36 h total
Result: Exhibited an EC50 of 1.85 μM against SFTSV infection in Vero cells, with a CC50 >300.0 μM and a selectivity index (SI) >162.16.

Real Time qPCR[1]

Cell Line: Vero cells infected with HRTV or GTV; BHK-21 cells infected with LCMV
Concentration: Gradient dilutions
Incubation Time: 1 h pre-incubation, then continuous treatment for 36 h total
Result: Exhibited EC50 values of 9.21 μM against HRTV in Vero cells, 4.21 μM against GTV in Vero cells, and 2.61 μM against LCMV in BHK-21 cells.
Parmacokinetics
Species Dose Route T1/2 Cmax Bioavailability
Rat[1] 20 mg/kg i.v. 0.5±0.115 h 20500±2610 ng/mL /
Rat[1] 20 mg/kg i.g. 5.23±0.84 h 14.7±18.4 ng/mL 0.5±0.09 %
In Vivo

Licoflavone C (20 mg/kg; intravenous injection; once daily for 3 consecutive days) significantly reduces SFTSV viral loads in the liver, spleen and blood of C57BL/6 J mice infected with SFTSV[1].
Licoflavone C (5-20 mg/kg; intravenous injection; once every 12 hours; for 2 consecutive days) dose-dependently reduces the SFTSV viral load in the liver, spleen, kidney and blood of C57BL/6 J mice infected with SFTSV[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 J (female, 6-8 weeks of age, challenged with SFTSV via intraperitoneal injection)[1]
Dosage: 20 mg/kg
Administration: i.v.; once daily; 3 consecutive days
Result: Reduced SFTSV viral loads in the liver, spleen, and blood significantly compared to vehicle control.
Showed no significant reduction in kidney viral loads.
Animal Model: C57BL/6 J (female, 6-8 weeks of age, challenged with SFTSV via intraperitoneal injection)[1]
Dosage: 5 mg/kg; 10 mg/kg; 20 mg/kg
Administration: i.v.; every 12 hours; 2 days
Result: Reduced SFTSV viral loads in the liver, spleen, kidney, and blood in a dose-dependent manner.
Reduced SFTSV viral loads across all four analyzed tissues significantly at 20 mg/kg.
Reduced SFTSV viral loads in the spleen, kidney, and blood significantly at 10 mg/kg.
Reduced SFTSV viral load in the spleen only significantly at 5 mg/kg.
Reduced SFTSV nucleoprotein fluorescence intensity in spleen tissue, consistent with viral load reductions.
Molecular Weight

338.35

Formula

C20H18O5
CAS No.
Appearance

Solid

Color

Light yellow to brown

SMILES

O=C1C=C(C2=CC=C(O)C=C2)OC3=C(C/C=C(C)\C)C(O)=CC(O)=C13
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (184.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9555 mL 14.7776 mL 29.5552 mL
5 mM 0.5911 mL 2.9555 mL 5.9110 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.83%

SDS (393 KB)

COA (250 KB) HNMR (266 KB) LCMS (89 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9555 mL 14.7776 mL 29.5552 mL 73.8880 mL
5 mM 0.5911 mL 2.9555 mL 5.9110 mL 14.7776 mL
10 mM 0.2956 mL 1.4778 mL 2.9555 mL 7.3888 mL
15 mM 0.1970 mL 0.9852 mL 1.9703 mL 4.9259 mL
20 mM 0.1478 mL 0.7389 mL 1.4778 mL 3.6944 mL
25 mM 0.1182 mL 0.5911 mL 1.1822 mL 2.9555 mL
30 mM 0.0985 mL 0.4926 mL 0.9852 mL 2.4629 mL
40 mM 0.0739 mL 0.3694 mL 0.7389 mL 1.8472 mL
50 mM 0.0591 mL 0.2956 mL 0.5911 mL 1.4778 mL
60 mM 0.0493 mL 0.2463 mL 0.4926 mL 1.2315 mL
80 mM 0.0369 mL 0.1847 mL 0.3694 mL 0.9236 mL
100 mM 0.0296 mL 0.1478 mL 0.2956 mL 0.7389 mL
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Licoflavone C Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Licoflavone C72357-31-4ApoptosisCaspaseEstrogen Receptor/ERRchromosome damagesevere fever with thrombocytopenia syndromeVero cellsSFTSV CENHRTV CENapoptosisGTV CENC57BL/6 J micehuman peripheral lymphocytesLCMV CENInhibitorinhibitorinhibit

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