Mal-Phe-C4-VC-PAB-MMAE
Based on 1 Customer Validation
Mal-Phe-C4-VC-PAB-MMAE is a drug-linker conjugate for ADC composed of MMAE conjugated to the Mal-Phe-C4-VC-PAB linker. Mal-Phe-C4-VC-PAB-MMAE can be used to synthesize ROR1-targeting antibody-drug conjugates (ADCs) for research on ROR1-expressing cancers.
For research use only. We do not sell to patients.
- Purity : 99.54%
- CAS No.: 2259318-51-7
- Formula: C72H105N11O15
- Molecular Weight:1364.67
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Storage:
-20°C, sealed storage, away from moisture and light
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Drug-Linker Conjugates for ADC Isoforms
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Biological Activity
Description
IC50 & Target
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Auristatin |
In Vitro
Mal-Phe-C4-VC-PAB-MMAE conjugated ADC-M (0.0045-10 μM; 72 h) exhibits potent antiproliferative activity against ROR1-positive Mino mantle cell lymphoma cells (EC50 = 15.8 nM), MDA-MB-468 triple-negative breast cancer cells (EC50 = 53.1 nM), and MCF-7 estrogen-positive breast cancer cells (EC50 = 164.1 nM)[1].
Mal-Phe-C4-VC-PAB-MMAE conjugated ADC-M undergoes rapid internalization into ROR1-positive Jeko-1 mantle cell lymphoma cells and MDA-MB-231 triple-negative breast cancer cells after binding to the cell surface ROR1 antigen[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2259318-51-7
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Appearance Solid
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Molecular Weight 1364.67
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Formula C72H105N11O15
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Color White to light yellow
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SMILES
O[C@@H](C1=CC=CC=C1)[C@@H](C)NC([C@H](C)[C@H]([C@@]2([H])N(CCC2)C(C[C@@H](OC)[C@@]([C@@H](C)CC)([H])N(C)C([C@H](C(C)C)NC([C@H](C(C)C)N(C)C(OCC3=CC=C(NC([C@H](CCCNC(N)=O)NC([C@H](C(C)C)NC(CCCC4=CC=C(N5C(C=CC5=O)=O)C=C4)=O)=O)=O)C=C3)=O)=O)=O)=O)OC)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* The compound is unstable in solutions, freshly prepared is recommended.
Solvent & Solubility
In Vitro:
DMSO : 200 mg/mL (146.56 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (3.66 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * The compound is unstable in solutions, freshly prepared is recommended.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (303 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7328 mL | 3.6639 mL | 7.3278 mL | 18.3194 mL |
| 5 mM | 0.1466 mL | 0.7328 mL | 1.4656 mL | 3.6639 mL | |
| 10 mM | 0.0733 mL | 0.3664 mL | 0.7328 mL | 1.8319 mL | |
| 15 mM | 0.0489 mL | 0.2443 mL | 0.4885 mL | 1.2213 mL | |
| 20 mM | 0.0366 mL | 0.1832 mL | 0.3664 mL | 0.9160 mL | |
| 25 mM | 0.0293 mL | 0.1466 mL | 0.2931 mL | 0.7328 mL | |
| 30 mM | 0.0244 mL | 0.1221 mL | 0.2443 mL | 0.6106 mL | |
| 40 mM | 0.0183 mL | 0.0916 mL | 0.1832 mL | 0.4580 mL | |
| 50 mM | 0.0147 mL | 0.0733 mL | 0.1466 mL | 0.3664 mL | |
| 60 mM | 0.0122 mL | 0.0611 mL | 0.1221 mL | 0.3053 mL | |
| 80 mM | 0.0092 mL | 0.0458 mL | 0.0916 mL | 0.2290 mL | |
| 100 mM | 0.0073 mL | 0.0366 mL | 0.0733 mL | 0.1832 mL |
Mal-Phe-C4-VC-PAB-MMAE Related Classifications
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