MAO-B-IN-60
MAO-B-IN-60 is a selective MAO-B inhibitor with blood-brain barrier permeability, with an IC50 of 0.07 nM. MAO-B-IN-60 exhibits region-specific MAO-B binding ability in rat brains. MAO-B-IN-60 can be used for the research of neurodegenerative diseases.
For research use only. We do not sell to patients.
- Formula: C18H16F3N3O2
- Molecular Weight:363.33
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
MAO-B 0.07 nM (IC50) |
In Vitro
MAO-B-IN-60 (Compound 32) potently inhibits recombinant human MAO-B, with over 10000-fold selectivity over MAO-A[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
[18F]MAO-B-IN-60 (96.2−122.1 MBq; intravenous injection; single administration) exhibits brain region-specific accumulation matching the distribution of MAO-B in normal SD rats[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley (SD) rats (male, 5-11 weeks old, 152-372 g)[1]
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Dosage:7.90-14.39 MBq (corresponding to 0.297-0.422 nM/kg)
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Administration:i.v.; single dose
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Result:Reached a peak whole-brain uptake of 1.40 SUV within 1 minute post-injection in baseline scans.
Decreased to 0.91 SUV at 25 minutes post-injection in baseline scans.
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Animal Model:Sprague-Dawley (SD) rats (male, 11 weeks old, 298-372 g)[1]
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Dosage:96.2-122.1 MBq
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Administration:i.v.; single dose
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Result:Exhibited widespread distribution in the cortex and pronounced accumulation in MAO-B-rich regions including the cerebellum, cingulum, lacunosum-moleculare of the hippocampus, and ependymal cells of the lateral and fourth ventricles.
Chemical Information
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Molecular Weight 363.33
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Formula C18H16F3N3O2
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SMILES
FC(C=C1NC(C2=CC=C3C(C=NN3CCOCCF)=C2)=O)=C(C=C1)F
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Transepithelial/transendothelial electrical resistance assay
TEER measures electrical resistance across epithelial or endothelial monolayers cultured on permeable supports, and the readout reflects ionic conductance through the cell barrier, especially the paracellular pathway regulated by junctional integrity. TEER can be measured without destroying the monolayer and is commonly used before or during transport, permeability, barrier-disruption, and barrier-maturation experiments. TEER values are influenced by biological maturation and technical conditions; reported factors include temperature, medium formulation, passage number, electrode geometry, membrane properties, and junctional length during early monolayer maturation. Therefore, TEER should be interpreted with blank-insert subtraction, area normalization, repeated readings, and, when possible, orthogonal barrier readouts such as FITC-dextran flux or tight-junction staining.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
MAO-B-IN-60 Related Classifications
HY-184917 Related Classifications
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